Allele Dmel\Nc³⁰

General Information
Symbol	Dmel\Nc³⁰	Species	D. melanogaster
Name		FlyBase ID	FBal0239865
Feature type	allele	Associated gene	Dmel\Nc

Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Waldhuber et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Imprecise excision of P{SUPor-P}Nc[KG02994] has deleted about 2.8 kb of genomic sequence, including the most of the sequence encoding Nc and CG6685. (Waldhuber et al., 2005)
Caused by aberration	Df(3L)Nc³⁰ (Waldhuber et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell death \| larval stage (Waldhuber et al., 2005) developmental rate defective \| larval stage (Waldhuber et al., 2005) lethal (with Df(3L)AC1) (Waldhuber et al., 2005) lethal (with Nc¹¹) (Waldhuber et al., 2005) lethal \| larval stage (Waldhuber et al., 2005) size defective \| larval stage (Waldhuber et al., 2005)
Phenotype Manifest In
	neuroblast & larval abdominal segment 3 \| late third instar larval stage (Waldhuber et al., 2005) neuroblast & larval abdominal segment 4 \| late third instar larval stage (Waldhuber et al., 2005) neuroblast & larval abdominal segment 5 \| late third instar larval stage (Waldhuber et al., 2005) neuroblast & larval abdominal segment 6 \| late third instar larval stage (Waldhuber et al., 2005) neuroblast & larval abdominal segment 7 \| late third instar larval stage (Waldhuber et al., 2005)
Detailed Description
	Statement Reference Nc[30] homozygous embryos hatch at the normal time but the larvae develop more slowly than wild-type. As a result, average body length at 72 hours after egg laying is 23% less than the average for Nc[30]/+ controls. These larvae fail to pupariate, but continue to grow and reach a final body mass 32% greater, on average, than control larvae just prior to pupariation. The resulting giant larvae die at 10-12 days after wild-type larvae pupariate. Unlike wild-type larvae, these larvae are not induced to pupariate early by feeding with ecdysone at the mid-third instar. Nc[30] homozygous larvae fail to exhibit cell death in the larval midgut, as wild-type larvae do, at end of the third larval instar (assayed by Tunel staining) or in response to feeding with ecdysone at mid-third instar (assayed by acridine orange staining). In Nc[30] homozygous larvae, most of the abdominal neuroblasts in segments A3-A7 of the CNS that normally die during the late third instar persist. X-ray induced apoptosis in wing discs is almost eliminated in Nc[30] homozygous third instar larvae. (Waldhuber et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference Nc³⁰/Nc[+] is a suppressor \| partially of eye phenotype of grim^GMR.PH (Waldhuber et al., 2005) Nc³⁰/Nc[+] is a suppressor \| partially of eye phenotype of rpr^GMR.PH (Waldhuber et al., 2005)
NOT Suppressor of
Statement Reference Nc³⁰/Nc[+] is a non-suppressor of eye phenotype of W^GMR.PG/W^GMR.PG (Waldhuber et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Nc[30]/+ partially suppresses the small, rough eye phenotype caused by rpr[GMR.PH] or grim[GMR.PH] but not that caused by W[GMR.PG]. (Waldhuber et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	Nc³⁰ is partially rescued by Nc^{Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^hs.PB (Waldhuber et al., 2005)
Not rescued by	Nc³⁰ is not rescued by Nc^{C318S.Scer\UAS}/Scer\GAL4^hs.PB (Waldhuber et al., 2005)
Comments	Pupariation of Nc[30] homozygous larvae is rescued in 85% of cases by Nc[Scer\UAS.T:Avic\GFP-EGFP]; Scer\GAL4[hs.PB]. It also rescues normal patterns of cell death in abdominal CNS neuroblasts during the late third instar and X-ray induced cell death in wing discs. (Waldhuber et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	DRONC³⁰ (Waldhuber et al., 2005) Nc³⁰
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Waldhuber et al., 2005, Mech. Dev. 122(7-8): 914--927 The Drosophila caspase DRONC is required for metamorphosis and cell death in response to irradiation and developmental signals. [FBrf0187988]

Allele Dmel\Nc30

Allele Dmel\Nc³⁰