FlyBase Allele Report: Dmel\sd[GA.UAS.Tag:HA]

General Information

Symbol

Dmel\sd^{GA.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0239888

Feature type

allele

Associated gene

Dmel\sd

Associated Insertion(s)

Carried in Construct

P{UAS-sd.GA.HA}

Also Known As

UAS-Sd-GA

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhang et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-sd.GA.HA}

Cytology

Description

Tagged with Tag:HA.

(Jin et al., 2013)

UAS regulatory sequences drive expression of a constitutively active form of sd, in which sd is fused to the Scer\GAL4 activation domain.

(Zhang et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Cho et al., 2018)

abnormal size | third instar larval stage, with Scer\GAL4^ptc-559.1

(Mach et al., 2018)

increased cell number | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2013)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2013)

visible, with Scer\GAL4^GMR.PF

(Zhang et al., 2008)

visible | adult stage, with Scer\GAL4^GMR.PF

(Cho et al., 2018)

Phenotype Manifest In

anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

(Mach et al., 2018)

eye, with Scer\GAL4^GMR.PF

(Cho et al., 2018, Zhang et al., 2008)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Jin et al., 2013)

wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

(Mach et al., 2018)

wing pouch | third instar larval stage, with Scer\GAL4^ptc-559.1

(Mach et al., 2018)

Detailed Description

Statement

Reference

Expression of sd^{GA.UAS.Tag:HA} under the control of Scer\GAL4^GMR.PF leads to an increase in eye size when compared to controls.

(Cho et al., 2018)

Expressing sd^{GA.UAS.Tag:HA} under the control of Scer\GAL4^ptc-559.1 stimulates tissue overgrowth in the wing disc, impairs wing pouch formation and results in a significant expansion of the ptc-positive anterior-posterior compartment boundary of the wing disc in third instar larvae.

(Mach et al., 2018)

An increased number of intestinal stem cells (ISCs) and enteroblasts are seen in the posterior midguts of adult flies expressing sd^{GA.Scer\UAS.T:Scer\GAL4-GAD.T:Ivir\HA1} under the control of Scer\GAL4^esg-NP5130. The number of mitotic cells is also increased but intestinal hyperplasia is not seen.

(Jin et al., 2013)

Expression of sd^{GA.Scer\UAS.T:Scer\GAL4-GAD} under the control of Scer\GAL4^GMR.PF results in an overgrown eye phenotype.

(Zhang et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, sd^{GA.UAS.Tag:HA} has abnormal size | adult stage phenotype, non-enhanceable by Prp4k^KK100483, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, sd^{GA.UAS.Tag:HA} has visible | adult stage phenotype, non-enhanceable by Prp4k^KK100483, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, sd^{GA.UAS.Tag:HA} has abnormal size | third instar larval stage phenotype, suppressible | partially by Hrb27C^GD6964, Scer\GAL4^ptc-559.1

(Mach et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, sd^{GA.UAS.Tag:HA} has visible phenotype, non-suppressible by hpo^{N.UAS.Tag:FLAG}/Scer\GAL4^GMR.PF

(Zhang et al., 2008)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, sd^{GA.UAS.Tag:HA} has eye phenotype, non-enhanceable by Prp4k^KK100483, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, sd^{GA.UAS.Tag:HA} has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by Hrb27C^GD6964, Scer\GAL4^ptc-559.1

(Mach et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, sd^{GA.UAS.Tag:HA} has eye phenotype, non-suppressible by hpo^{N.UAS.Tag:FLAG}/Scer\GAL4^GMR.PF

(Zhang et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of brm^GD4507 greatly suppresses the intestinal stem cell overproliferation seen when sd^{GA.Scer\UAS.T:Scer\GAL4-GAD.T:Ivir\HA1} is expressed in adult flies under the control of Scer\GAL4^esg-NP5130.

(Jin et al., 2013)

The overgrown eye phenotype caused by expression of sd^{GA.Scer\UAS.T:Scer\GAL4-GAD} under the control of Scer\GAL4^GMR.PF is not suppressed by hpo^{N.Scer\UAS.T:Zzzz\FLAG}.

(Zhang et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sd^{GA.Scer\UAS.T:Scer\GAL4-GAD.T:Ivir\HA1}

sd^{GA.Scer\UAS.T:Scer\GAL4-GAD}

sd^{GA.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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