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General Information
Symbol
Dmel\ykiUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zhang
FlyBase ID
FBal0239893
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Yki
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of yki.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of ykiUAS.cZa under the control of Scer\GAL4moody.PU induces a compromised blood-brain barrier, as dye injected into the third instar larval body cavity is able to diffuse into the brain. In agreement, larval subperineurial glial cells show disrupted/discontinuous septate junctions (Dlg immunostaining) and significant increases in nuclear size and ploidy, as compared to controls. There is also a significant increase in larval brain volume , as compared to controls.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU induces adult eye overgrowth.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4esg-NP5130 (restricted to the adult stages using Scer\GAL80ts.αTub84B) results in an increase in intestinal stem cell division.

Expression of ykiScer\UAS.cZa in enterocytes (ECs) under the control of Scer\GAL4Myo31DF-NP0001 (restricted to the adult stages using Scer\GAL80ts.αTub84B) results in an increase in intestinal stem cell division.

Intestinal stem cell clones expressing ykiScer\UAS.cZa contain more cells than control clones.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in enlarged eyes.

An increased number of intestinal stem cells (ISCs) and enteroblasts are seen in the posterior midguts of adult flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4esg-NP5130. The number of mitotic cells is also increased, resulting in pronounced hyperplasia of the intestine with a thicker intestinal epithelium composed of a multi-layer of tightly packed cells.

Overexpression of ykiScer\UAS.cZa in intestinal stem cell clones under the control of Scer\GAL4tub.PU results in a significant increase in cell numbers and in the formation of large clones.

Expression of ykiScer\UAS.cZa in adult enterocytes under the control of Scer\GAL4Myo31DF.PU results in an increased number of intestinal stem cells and enteroblasts.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in dramatic eye overgrowth.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in an enlarged eye.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in eye overgrowth.

Overexpression of ykiScer\UAS.cZa in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 leads to an increase in the number of precursor cells.

Overexpression of ykiScer\UAS.cZa in absorptive enterocytes in the adult midgut under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B induces a dramatic increase in intestine stem cell proliferation.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4hh-Gal4 results in increased size of the posterior compartment in the wing disc.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in overgrown eyes.

The eye overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of either ykidsRNA.N.Scer\UAS or ykidsRNA.C.Scer\UAS.

Expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF results in enlarged eyes.

Ectopic BrdU incorporation posterior to the second mitotic wave is seen in the eye discs of animals expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The eye overgrowth observed in adult flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU is increased further by co-expression of AckScer\UAS.T:SV5\V5 (which on its own also promotes eye growth) but not AckKD.Scer\UAS.T:SV5\V5.

Expression of bansponge.Scer\UAS.T:Disc\RFP does not suppress the increase in intestinal stem cell division when ykiScer\UAS.cZa is expressed in enterocytes (ECs) under the control of Scer\GAL4Myo31DF-NP0001 (restricted to the adult stages using Scer\GAL80ts.αTub84B).

Expression of bansponge.Scer\UAS.T:Disc\RFP suppresses the increase in intestinal stem cell division seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4esg-NP5130 (restricted to the adult stages using Scer\GAL80ts.αTub84B).

Df(3L)banΔ1 suppresses the increase in intestinal stem cell clone size seen when ykiScer\UAS.cZa is expressed.

Expression of par-1L-484 enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

Expression of par-1L-507 enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

Expression of par-1F-727 enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

Expression of par-1Scer\UAS.T:Hsap\MYC.cHa enhances the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

Expression of par-1KD.Scer\UAS.T:Hsap\MYC does not dramatically enhance the eye overgrowth phenotype seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

Expression of ykiScer\UAS.cZa suppresses the shrunken P-compartment phenotype seen in wing discs when par-1HMS00405 is expressed under the control of Scer\GAL4hh-Gal4.

Expression of brmGD4507 greatly suppresses the intestinal stem cell overproliferation and hyperplasia seen when ykiScer\UAS.cZa is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

brm2 completely suppresses the significant increase in cell numbers seen in intestinal stem cell clones overexpressing of ykiScer\UAS.cZa under the control of Scer\GAL4tub.PU.

Expression of ykiScer\UAS.cZa enhances the increased intestinal stem cell proliferation seen when brmD718A.Scer\UAS.T:Zzzz\FLAG is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

Expression of brmN.Scer\UAS.T:Zzzz\FLAG enhances the increased intestinal stem cell proliferation seen when ykiScer\UAS.cZa is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

Expression of brmC.Scer\UAS.T:Zzzz\FLAG enhances the increased intestinal stem cell proliferation seen when ykiScer\UAS.cZa is expressed in adult flies under the control of Scer\GAL4esg-NP5130.

Expression of brmScer\UAS.T:Zzzz\FLAG does not significantly affect the eye overgrowth seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

Expression of brmD718A.Scer\UAS.T:Zzzz\FLAG enhances the eye overgrowth seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PU.

Co-expression of hpoScer\UAS.WT.T:Zzzz\FLAG and ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in small, rough eyes.

Co-expression of hpoT195E.Scer\UAS.T:Zzzz\FLAG and ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in small, rough eyes.

Co-expression of hpoI634D.Scer\UAS.T:Zzzz\FLAG suppresses the overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU, with the resulting flies having rough eyes.

Co-expression of hpoK71R.Scer\UAS.T:Zzzz\FLAG in flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU results in a dramatic increase in eye size.

Co-expression of either hpoT195A.Scer\UAS.T:Zzzz\FLAG, hpoM242E.Scer\UAS.T:Zzzz\FLAG or hpoΔ238-246.Scer\UAS.T:Zzzz\FLAG enhances the eye overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PU.

Expression of edGD2575 enhances the eye overgrowth seen when ykiScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF.

Coexpression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with ykiScer\UAS.cZa in adult intestine stem cells and enteroblasts using Scer\GAL4esg-NP5130 suppresses the intestine stem cell proliferation seen upon expression of ykiScer\UAS.cZa alone.

Co-expression of sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS with ykiScer\UAS.cZa in absorptive enterocytes in the adult midgut under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B suppresses the proliferation increase in intestine stem cells seen upon expression of ykiScer\UAS.cZa alone.

The eye overgrowth phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is enhanced by co-expression of sdScer\UAS.T:Ivir\HA1. The eyes are depigmented in these flies.

The amount of ectopic BrdU incorporation posterior to the second mitotic wave that is seen in the eye discs of flies expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is enhanced by co-expression of sdScer\UAS.T:Ivir\HA1.

The enlarged eye phenotype caused by expression of ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of either sddsRNA.N.Scer\UAS or sddsRNA.C.Scer\UAS (or both co-expressed together). The ectopic incorporation of BrdU seen in the eye discs of animals expressing ykiScer\UAS.cZa under the control of Scer\GAL4GMR.PF is also suppressed by simultaneous co-expression of both sddsRNA.N.Scer\UAS and sddsRNA.C.Scer\UAS.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ykiScer\UAS.cZa
ykiUAS.cZa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Zhang
Secondary FlyBase IDs
    References (10)