FB2025_01 , released February 20, 2025
Allele: Dmel\nopoZ1447
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General Information
Symbol
Dmel\nopoZ1447
Species
D. melanogaster
Name
FlyBase ID
FBal0239900
Feature type
allele
Associated gene
Dmel\nopo
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Merkle et al., 2009)
Mutagen
ethyl methanesulfonate
(Merkle et al., 2009)
Progenitor genotype
Cytology
Description

Amino acid replacement: E11K.

(Merkle et al., 2009)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:18,180,752..18,180,752 [+]
Nucleotide change:

G18180752A

Amino acid change:

E11K | nopo-PA; E11K | nopo-PB

Reported amino acid change:

E11K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Merkle et al., 2009)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The egg chambers of mutant females show no obvious defects in oogenesis, and polar bodies are present in their unfertilised eggs (indicating that meiosis is completed).

      72% of embryos derived from homozygous females mated to wild-type males develop to cycle 10 or beyond, but none of these embryos develop to the initiation of gastrulation or beyond. The embryos have unevenly spaced nuclei. The mitotic spindles are shortened and barrel-shaped (64%) and have detached centrosomes (62%) and misaligned chromosomes. There is asynchrony between nuclear and centrosome cycles, with centrosome duplication before telophase occasionally being evident (13% of spindles have more than one centrosome per pole). 10% of the spindles are tripolar.

      68% of embryos derived from nopoZ1447/Df(2R)Exel7153 females mated to wild-type males develop to cycle 10 or beyond, but none of these embryos develop to the initiation of gastrulation or beyond.

      (Merkle et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      nopoZ1447 has lethal | recessive | non-rescuable maternal effect | embryonic stage phenotype, non-suppressible by lokp6

      (Merkle et al., 2009)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      nopoZ1447 has spindle | non-rescuable maternal effect phenotype, suppressible by lokp6

      (Merkle et al., 2009)

      nopoZ1447 has centrosome | non-rescuable maternal effect phenotype, suppressible by lokp6

      (Merkle et al., 2009)
      Enhancer of
      Other
      Statement
      Reference

      lokp6, nopoZ1447 has interphase & organism | embryonic cycle 11 | maternal effect phenotype

      (Merkle et al., 2009)

      nopoZ1447/Df(2R)Exel7153, lokp6 has interphase & organism | embryonic cycle 11 | maternal effect phenotype

      (Merkle et al., 2009)

      lokp6, nopoExc142/nopoZ1447 has interphase & organism | embryonic cycle 11 | maternal effect phenotype

      (Merkle et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      lokp6 partially suppresses the phenotype of embryos derived from nopoZ1447 females; the acentrosomal, barrel-shaped mitotic spindles are restored to normal spindles with attached centrosomes. However, DNA defects are common in embryos derived from the double mutant females, particularly during cortical divisions, with abnormal DNA aggregates that are shared by more than one spindle often being seen. The embryos derived from the double mutant females complete syncytial divisions, and arrest with aberrant morphology upon the initiation of gastrulation.

      Embryos derived from lokp6 nopoZ1447 double mutant females have significantly shorter interphases during cycle 11 compared to wild-type or lokp6 single mutant embryos.

      Embryos derived from lokp6 nopoZ1447/lokp6 Df(2R)Exel7153 females have significantly shorter interphases during cycle 11 compared to wild-type or lokp6 single mutant embryos.

      Embryos derived from lokp6 nopoZ1447/lokp6 nopoExc142 females have significantly shorter interphases during cycle 11 compared to wild-type or lokp6 single mutant embryos.

      (Merkle et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      nopoZ1447 is rescued by nopo+t3.8

      (Merkle et al., 2009)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TraipZ1447
      (O'Neill and Rusan, 2022)
      nopoZ1447
      (Ketosugbo et al., 2017, Merkle et al., 2009)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)