Allele Dmel\Traf4^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Traf4^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0239950
Feature type	allele	Associated gene	Dmel\Traf4

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Igaki et al., 2006) in vitro construct - RNAi (Igaki et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive Traf4 coding sequences in an inverted repeat to allow cell-targeted generation of dsRNA for RNAi knockdown experiments. (Igaki et al., 2006)
Carried in construct	P{UAS-Traf1.IR} (Igaki et al., 2006, Wu et al., 2009, Xue et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a suppressor \| partially of visible phenotype of Ras85D^KP (Wu et al., 2009) Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of BacA\p35^GMR.PH, Ras85D^KP (Wu et al., 2009)
NOT Suppressor of
Statement Reference Traf4^{dsRNA.Scer\UAS}, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI is a non-suppressor of tumorigenic \| somatic clone phenotype of Ras85D^V12.Scer\UAS, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, l(2)gl⁴ (Igaki et al., 2006) Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa (Xue et al., 2007)
Phenotype Manifest In
Suppressor of
Statement Reference Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a suppressor \| partially of eye phenotype of Ras85D^KP (Wu et al., 2009) Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of BacA\p35^GMR.PH, Ras85D^KP (Wu et al., 2009)
NOT Suppressor of
Statement Reference Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa (Xue et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference The eye phenotype in Ras85D[KP] mutant flies is partially suppressed by ectopic expression of Traf4[dsRNA.Scer\UAS] in the eye under the control of Scer\GAL4[GMR.PF]. (Wu et al., 2009) The small-eye phenotype caused by expression of egr[Scer\UAS.cIa] under the control of Scer\GAL4[GMR.PF] is not suppressed by co-expression of Traf4[dsRNA.Scer\UAS]. (Xue et al., 2007) Expression of Traf4[dsRNA.Scer\UAS] (under the control of Scer\GAL4[Act5C.PI]) using the FLP/FRT system fails to suppress metastatic behaviour in l(2)gl[4] mutants expressing Ras85D[V12.Scer\UAS]. (Igaki et al., 2006)
Xenogenetic Interactions
Statement Reference Expression of Traf4[dsRNA.Scer\UAS] under the control of Scer\GAL4 in flies expressing BacA\p35[GMR.PH] does not result in an eye phenotype. Expression of Traf4[dsRNA.Scer\UAS] (under the control of Scer\GAL4[GMR.PF]) and BacA\p35[GMR.PH] in a Ras85D[KP] mutant background strongly suppresses the eye phenotype. (Wu et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Traf4^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Wu et al., 2009, BMC Dev. Biol. 9: 53 Ras promotes cell survival by antagonizing both JNK and Hid signals in the Drosophila eye. [FBrf0209242] Xue et al., 2007, Dev. Cell 13(3): 446--454 Tumor suppressor CYLD regulates JNK-Induced cell death in Drosophila. [FBrf0200559] Igaki et al., 2006, Curr. Biol. 16(11): 1139--1146 Loss of cell polarity drives tumor growth and invasion through JNK activation in Drosophila. [FBrf0194200]

Allele Dmel\Traf4dsRNA.Scer\UAS

Allele Dmel\Traf4^{dsRNA.Scer\UAS}