FlyBase Allele Report: Dmel\Csk[RNAi.UAS]

General Information

Symbol

Dmel\Csk^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0240158

Feature type

allele

Associated gene

Dmel\Csk

Associated Insertion(s)

Carried in Construct

P{UAS-Csk.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Vidal et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Vidal et al., 2006)

Mutagen

in vitro construct

(Vidal et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Csk.RNAi}

Cytology

Description

UAS sequences control expression of an inverted repeat of Csk, amplified using primers 5'-TGTCTTCACCAGCAAGCATC-3' and 5'-CTCCCTTGCTGACTCCTCAC-3', separated by w intron spacer sequence.

(Vidal et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Csk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

eye, with Scer\GAL4^GMR.PF

(Vidal et al., 2006)

eye, with Scer\GAL4^unspecified

(Lee et al., 2009)

interommatidial precursor cell | pupal stage, with Scer\GAL4^GMR.PF

(Vidal et al., 2006)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Cordero et al., 2014)

ommatidium, with Scer\GAL4^GMR.PF

(Vidal et al., 2006)

secondary pigment cell | increased number, with Scer\GAL4^GMR.PF

(Vidal et al., 2006)

tertiary pigment cell | increased number, with Scer\GAL4^GMR.PF

(Vidal et al., 2006)

wing, with Scer\GAL4^C-765

(Vidal et al., 2006)

wing, with Scer\GAL4^sd-SG29.1

(Vidal et al., 2006)

wing disc, with Scer\GAL4^ptc-559.1

(Das et al., 2013, Langton et al., 2007, Vidal et al., 2006)

wing vein | ectopic, with Scer\GAL4^C-765

(Vidal et al., 2006)

wing vein | ectopic, with Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

wing vein L4, with Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

zonula adherens & disc epithelium proper, with Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

Detailed Description

Statement

Reference

Scer\GAL4^esg-NP5130-mediated expression of Src64B^Scer\UAS.cCa (restricted to adult stages by using P{tubP-GAL80^ts}) results in substantial intestinal stem cell hyperproliferation and hyperplasia of the adult intestine.

(Cordero et al., 2014)

Expression of Csk^{dsRNA.Scer\UAS} (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^ptc-559.1 results in mild but consistent cell migration from the ptc domain of the wing disc compared to controls.

(Das et al., 2013)

Expression of Csk^IR.Scer\UAS using Scer\GAL4^unspecified causes a weak mispatterning phenotype in the adult eye.

(Lee et al., 2009)

Expression of Csk^IR.Scer\UAS under the control of Scer\GAL4^ptc-559.1 in the wing disc resulting in the delamination, movement and apoptosis of some of the mutant cells along the boundary between the mutant and wild-type cells.

(Langton et al., 2007)

Csk^IR.Scer\UAS; Scer\GAL4^GMR.PF adults have enlarged, rough eyes. Analysis of the developing retinas of these animals at 42 hours after puparium formation shows an excess and mis-patterning of secondary and tertiary pigment cells. Even in areas of the retina with normal numbers of these cells, their arrangement is disrupted. Earlier still, at 29 hours after puparium formation, there is a striking reduction in apoptosis in the developing retina. In particular, excess inter-ommatidial precursor cells fail to die. Initially these precursor cells mostly take up a normal arrangement in the inter-ommatidial lattice, but they then fail to hold these positions - often sliding over each other to form piles rather than remaining as a monolayer.

Csk^IR.Scer\UAS; Scer\GAL4^Act5C.PI animals die as young pupae with a body size approximately 50% larger than wild-type controls.

Csk^IR.Scer\UAS; Scer\GAL4^C-765 adults have significantly larger wings than controls and have extra vein material in veins L2 and L5 and the posterior crossvein.

Csk^IR.Scer\UAS; Scer\GAL4^Act5C.PI somatic clones in the eye disc are smaller than their wild-type twin spots and rarely survive in the pupal retina. Cell death in these clones in concentrated at their boundaries.

The wings of Csk^IR.Scer\UAS; Scer\GAL4^sd-SG29.1 adults are severely reduced and malformed. In contrast, the wings of Csk^IR.Scer\UAS; Scer\GAL4^ptc-559.1 adults are normal in size, but have ectopic vein material associated with wing vein L4 and a missing or defective anterior crossvein. The wing discs of these animals contain increased numbers of apoptotic cells at the posterior edge of the ptc expression domain. In addition, cells in a region extending 3-4 cell diameters anterior to the anterior-posterior boundary lose components of their zonula adherens and then lose their apical profiles. These cells often delaminate completely from the wing disc epithelium and some end up on the posterior side of the compartment boundary, despite their origins in the anterior compartment. No increase in cell death or loss of apical profiles is seen in Csk^IR.Scer\UAS; Scer\GAL4^bi-omb-Gal4 wing discs.

(Vidal et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has abnormal cell migration phenotype, enhanceable by Sin3A^KK100700, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, enhanceable by shg[+]/shg^R69

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, enhanceable by puc^E69/puc[+]

(Vidal et al., 2006)

Suppressed by

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700 has abnormal cell migration phenotype, suppressible by bsk^K53R.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has lethal | pupal stage phenotype, suppressible by ASPP^UASp.cLa, Scer\GAL4^ptc-559.1

(Langton et al., 2007)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, suppressible by puc^UAS.cMa

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, suppressible by Rho1^72O/Rho1[+]

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has increased cell death phenotype, suppressible by p120ctn[+]/p120ctn³⁰⁸

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^sd-SG29.1 has increased cell death phenotype, suppressible by puc^UAS.cMa

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^sd-SG29.1 has increased cell death phenotype, suppressible | partially by p120ctn[+]/p120ctn³⁰⁸

(Vidal et al., 2006)

Enhancer of

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 is an enhancer of abnormal cell migration phenotype of Scer\GAL4^ptc-559.1, Sin3A^KK100700

(Das et al., 2013)

Other

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700, bsk^K53R.UAS has abnormal size phenotype

(Das et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has wing disc phenotype, enhanceable by Sin3A^KK100700, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700 has wing disc phenotype, enhanceable by bsk^K53R.UAS, Scer\GAL4^ptc-559.1

(Das et al., 2013)

Csk^RNAi.UAS, Scer\GAL4^unspecified has eye phenotype, enhanceable by HPS4^unspecified

(Lee et al., 2009)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has wing disc phenotype, enhanceable by ASPP⁸/ASPP⁸

(Langton et al., 2007)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has zonula adherens & disc epithelium proper phenotype, enhanceable by puc^E69/puc[+]

(Vidal et al., 2006)

Suppressed by

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^unspecified has eye phenotype, suppressible by Dcr-2^unspecified

(Lee et al., 2009)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has zonula adherens & disc epithelium proper phenotype, suppressible by puc^UAS.cMa

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has zonula adherens & disc epithelium proper phenotype, suppressible by Rho1^72O/Rho1[+]

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has zonula adherens & disc epithelium proper phenotype, suppressible by shg[+]/shg^R69

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 has zonula adherens & disc epithelium proper phenotype, suppressible by p120ctn[+]/p120ctn³⁰⁸

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^GMR.PF has secondary pigment cell | increased number phenotype, suppressible by shg^UAS.cSa

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^GMR.PF has tertiary pigment cell | increased number phenotype, suppressible by shg^UAS.cSa

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsap\MATK^UAS.cVa

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Hsap\MATK^UAS.cVa

(Vidal et al., 2006)

NOT suppressed by

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by p120ctn[+]/p120ctn³⁰⁸

(Vidal et al., 2006)

Csk^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by p120ctn[+]/p120ctn³⁰⁸

(Vidal et al., 2006)

Enhancer of

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1 is an enhancer of wing disc phenotype of Scer\GAL4^ptc-559.1, Sin3A^KK100700

(Das et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/Csk^RNAi.UAS is a suppressor of eye phenotype of rpr^GMR.PW

(Vidal et al., 2006)

Other

Statement

Reference

Csk^RNAi.UAS, Scer\GAL4^ptc-559.1, Sin3A^KK100700, bsk^K53R.UAS has wing disc phenotype

(Das et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Csk^{dsRNA.Scer\UAS} and Sin3A^KK100700 (with Dcr-2^Scer\UAS.cDa) results in a much stronger wing disc cell migration phenotype than is seen when either transgene is expressed alone. Upon co-expression of bsk^{K53R.Scer\UAS}, massive overgrowth is seen, but the invasion phenotype is suppressed.

(Das et al., 2013)

The mispatterning seen in Csk^IR.Scer\UAS, Scer\GAL4^unspecified eyes is: (i) restored when eyes are also made homozygous mutant for Dcr-2^unspecified, or (ii) strongly enhanced when eyes are also made homozygous mutant for HPS4^unspecified (using the "eyFLP/FRT" system).

(Lee et al., 2009)

ASPP⁸/ASPP⁸ dramatically enhances the delamination, movement and apoptosis phenotype seen in Csk mutant cells which are at the boundary between mutant and wild-type cells in wing discs expressing Csk^IR.Scer\UAS under the control of Scer\GAL4^ptc-559.1.

(Langton et al., 2007)

Csk^IR.Scer\UAS; Scer\GAL4^GMR.PF partially suppresses the reduction in eye size due to rpr^GMR.PW.

The extra secondary and tertiary pigment cell phenotype seen in the developing retinas of Csk^IR.Scer\UAS; Scer\GAL4^GMR.PF animals at 42 hours after puparium formation is suppressed by shg^Scer\UAS.cSa.

Rho1^72O/+ or puc^Scer\UAS.cMa or shg^R69/+ suppress the loss of apical profile, delamination and subsequent migration and cell death increase seen in cells at the posterior edge of theptc expression domain in Csk^IR.Scer\UAS Scer\GAL4^ptc-559.1 third instar larvae. p120ctn³⁰⁸/+ partially suppresses these phenotypes. In contrast, puc^E69/+ strongly enhances these phenotypes.

(Vidal et al., 2006)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype of Csk^IR.Scer\UAS; Scer\GAL4^GMR.PF adults is suppressed by Hsap\MATK^Scer\UAS.cVa.

(Vidal et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Csk^IR.Scer\UAS

Csk^RNAi.UAS

Csk^{dsRNA.Scer\UAS}

Csk^dsRNA.UAS

dCsk^RNAi

(Das et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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