FlyBase Allele Report: Dmel\Ilp2[UAS.cBa]

General Information

Symbol

Dmel\Ilp2^UAS.cBa

Species

D. melanogaster

Name

FlyBase ID

FBal0240162

Feature type

allele

Associated gene

Dmel\Ilp2

Associated Insertion(s)

Carried in Construct

P{UAS-Ilp2.B}

Also Known As

UAS-dilp2

Key Links

Transgenic product class

cDNA

(Brogiolo et al., 2001)

wild_type

(Brogiolo et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Brogiolo et al., 2001)

wild_type

(Brogiolo et al., 2001)

Mutagen

in vitro construct

(Hong et al., 2012, Ikeya et al., 2002, Brogiolo et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Ilp2.B}

Cytology

Description

UASt regulatory sequences drive expression of a full-length Ilp2 cDNA (GH11579 clone).

(Brogiolo et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ilp2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates obesity

modeled by Itpr^ka1091, Itpr^ug3

(Subramanian et al., 2013)

exacerbates cancer

modeled by scrib¹

(Sanaki et al., 2020)

ameliorates type 2 diabetes mellitus

modeled by Uch^KO

(Lee et al., 2024)

modeled by Uch^C93S

(Lee et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^hs.PB

(Keith et al., 2021)

abnormal feeding behavior, with Scer\GAL4^elav-C155

(Wu et al., 2005)

abnormal feeding behavior | adult stage, with Scer\GAL4^sNPF-R.2.5

(Hong et al., 2012)

abnormal neuroanatomy | adult stage | somatic clone, with Scer\GAL4^6-60

(Luo et al., 2020)

abnormal size, with Scer\GAL4^sd-SG29.1

(Tsuda et al., 2010)

abnormal stress response, with Scer\GAL4^Ilp2.PR

(Karpac et al., 2009)

chemical resistant, with Scer\GAL4^arm.PU

(McClure et al., 2011)

chemical resistant | larval stage, with Scer\GAL4^Ilp2.PR

(McClure et al., 2011)

increased body size

(Lee et al., 2008)

increased body size, with Scer\GAL4^arm.PS

(Honegger et al., 2008, Ikeya et al., 2002)

increased body size, with Scer\GAL4^hs.PB

(Brogiolo et al., 2001)

increased cell number, with Scer\GAL4^hs.PB

(Brogiolo et al., 2001)

increased cell size, with Scer\GAL4^hs.PB

(Brogiolo et al., 2001)

lethal, with Scer\GAL4^Act5C.PP

(Ikeya et al., 2002, Brogiolo et al., 2001)

lethal, with Scer\GAL4^ppl.PP

(Honegger et al., 2008)

short lived | nutrition conditional, with Scer\GAL4^Ilp2.PW

(Enell et al., 2010)

visible, with Scer\GAL4^hs.PB

(Brogiolo et al., 2001)

Phenotype Manifest In

dendrite | adult stage | somatic clone, with Scer\GAL4^6-60

(Luo et al., 2020)

distal medullary amacrine neuron Dm8 | adult stage | somatic clone, with Scer\GAL4^6-60

(Luo et al., 2020)

wing, with Scer\GAL4^sd-SG29.1

(Tsuda et al., 2010)

Detailed Description

Statement

Reference

Expression of Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^ey.PU has no effect on eye development.

(Nowak et al., 2013)

Flies expressing Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^sNPF-R.2.5 show significantly decreased food consumption compared to controls.

(Hong et al., 2012)

Expression of Ilp2^Scer\UAS.cBa in the insulin producing cells (IPCs) under the control of Scer\GAL4^Ilp2.PR does not ameliorate either the lethality or developmental delay phenotypes associated with developmental ethanol exposure. Ubiquitous expression under the control of Scer\GAL4^arm.PU does suppress the developmental phenotypes. Expression of Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^Ilp2.PR completely rescues the increased resistance to sedation and ability to develop tolerance seen in wild type larvae raised on 5% ethanol.

(McClure et al., 2011)

Male flies expressing Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^Ilp2.PW display a strongly reduced survival at starvation.

(Enell et al., 2010)

Mis-expression of Ilp2^Scer\UAS.cBa in developing wing imaginal discs produces adult flies with a wing size larger than that of control.

(Tsuda et al., 2010)

Flies expressing Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^Ilp2.PR show a significantly reduced repression of growth (assayed by measuring wing area) when shifted from 25[o]C to 29[o]C compared to wild-type flies.

Flies expressing Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^Ilp2.PR show increased sensitivity to oxidative stress sensitivity.

(Karpac et al., 2009)

Overexpression of Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^arm.PS results in increased body size and weight.

Overexpression of Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^ppl.PP causes lethality.

(Honegger et al., 2008)

Expression of Ilp2^Scer\UAS.cBa using Scer\GAL4^Ilp2.PR increases body size.

(Lee et al., 2008)

Larvae expressing Ilp2^Scer\UAS.cIa, under the control of Scer\GAL4^elav-C155 display a normal feeding response when fed ad libitum. The same third instar larvae fasted for 120 minutes display significantly attenuated feeding rates. Comparison to controls reveal that Ilp2^Scer\UAS.cIa flies (Scer\GAL4^elav-C155) ingest liquid and solid food approximately 30% slower.

(Wu et al., 2005)

When Ilp2^Scer\UAS.cIa is driven by Scer\GAL4^arm.PS a statistically significant increase in body size of about 51% is seen.

(Ikeya et al., 2002)

When Ilp2^Scer\UAS.cBa is expressed by under the control of Scer\GAL4^hs.PB, a proportionate increase in body size is seen. Compared to wild-type body weight and number of ommatidia are increased by 39% and 5% respectively. The wing areas is increased by 21% due to significant increases in cell size and number (9% and 11% respectively).

(Brogiolo et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ilp2^UAS.cBa, Scer\GAL4^arm.PS has increased body size phenotype, enhanceable by ImpL2[+]/ImpL2^Def42

(Honegger et al., 2008)

Suppressed by

Statement

Reference

Ilp2^UAS.cBa, Scer\GAL4^sd-SG29.1 has abnormal size phenotype, suppressible by Ide^UAS.cTa, Scer\GAL4^sd-SG29.1

(Tsuda et al., 2010)

Ilp2^UAS.cBa, Scer\GAL4^ppl.PP has lethal phenotype, suppressible by ImpL2^s.UAS, Scer\GAL4^ppl.PP

(Honegger et al., 2008)

Ilp2^UAS.cBa, Scer\GAL4^hs.PB has increased cell number phenotype, suppressible by InR³⁰⁴

(Brogiolo et al., 2001)

Ilp2^UAS.cBa, Scer\GAL4^hs.PB has increased cell size phenotype, suppressible by InR³⁰⁴

(Brogiolo et al., 2001)

Ilp2^UAS.cBa, Scer\GAL4^hs.PB has increased body size phenotype, suppressible by InR³⁰⁴

(Brogiolo et al., 2001)

Ilp2^UAS.cBa, Scer\GAL4^hs.PB has visible phenotype, suppressible by InR³⁰⁴

(Brogiolo et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Ilp2^UAS.cBa is a suppressor of abnormal starvation stress response | semidominant | adult stage phenotype of mir-137^CR

(Saedi et al., 2024)

Scer\GAL4^Ilp2.PR/Ilp2^UAS.cBa is a suppressor of abnormal starvation stress response | semidominant | adult stage phenotype of mir-137^CR

(Saedi et al., 2024)

Scer\GAL4^elav-C155/Ilp2^UAS.cBa is a suppressor of abnormal starvation stress response | semidominant | adult stage phenotype of mir-137^KO

(Saedi et al., 2024)

Scer\GAL4^Ilp2.PR/Ilp2^UAS.cBa is a suppressor of abnormal starvation stress response | semidominant | adult stage phenotype of mir-137^KO

(Saedi et al., 2024)

Ilp2^UAS.cBa, Scer\GAL4^elav-C155 is a suppressor of long lived phenotype of Scer\GAL4^elav-C155, dcma^GD15125

(Wilson et al., 2020)

Ilp2^UAS.cBa, Scer\GAL4^Ilp2.PR is a suppressor of long lived phenotype of Scer\GAL4^Ilp2.PR, dcma^GD15126

(Wilson et al., 2020)

Ilp2^UAS.cBa/Scer\GAL4^ey.PU is a suppressor of increased cell growth rate | somatic clone | nutrition conditional phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Scer\GAL4^Ilp2.PR/Ilp2^UAS.cBa is a suppressor of abnormal starvation stress response phenotype of Itpr^ug3/Itpr^ka1091

(Subramanian et al., 2013)

Scer\GAL4^Ilp2.PR/Ilp2^UAS.cBa is a suppressor of increased body size phenotype of Itpr^ug3/Itpr^ka1091

(Subramanian et al., 2013)

Scer\GAL4^Ilp2.PR/Ilp2^UAS.cBa is a suppressor of abnormal feeding behavior phenotype of Itpr^ug3/Itpr^ka1091

(Subramanian et al., 2013)

Scer\GAL4^arm.PS/Ilp2^UAS.cBa is a suppressor of viable phenotype of ImpL2^Def20/ImpL2^Def42

(Honegger et al., 2008)

NOT Suppressor of

Statement

Reference

Ilp2^UAS.cBa, Scer\GAL4^r4 is a non-suppressor of partially lethal - majority die phenotype of Scer\GAL4^r4, Tl^{10b.UAS.Tag:MYC}

(Suzawa et al., 2019)

Scer\GAL4^GMR.PB, Df(3L)AC1/Ilp2^UAS.cBa is a non-suppressor of visible phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PB

(Brogiolo et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ilp2^UAS.cBa, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible by Ide^UAS.cTa, Scer\GAL4^sd-SG29.1

(Tsuda et al., 2010)

Suppressor of

Statement

Reference

Ilp2^UAS.cBa/Scer\GAL4^ey.PU is a suppressor of eye | somatic clone | nutrition conditional phenotype of Pten¹¹⁷

(Nowak et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PB, Df(3L)AC1/Ilp2^UAS.cBa is a non-suppressor of eye phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PB

(Brogiolo et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^ey.PU suppresses the overgrowth found in Pten¹¹⁷ mutant clones under starvation conditions. Furthermore, expression of Ilp2^Scer\UAS.cBa restores the growth of both the tissue surrounding the Pten¹¹⁷ clone and peripheral tissue.

(Nowak et al., 2013)

Expression of Ilp2^Scer\UAS.cBa in the insulin-producing cells under the control of Scer\GAL4^Ilp2.PR rescues the starvation resistance and mobilization of TAGs from abdominal fat body cells seen in itgr[ku] (Itp-r83A^ka1091/Itp-r83A^ug3) mutant flies to wild type levels. The increase in body weight and stored TAG levels are only partially rescued.

Expression of Ilp2^Scer\UAS.cBa in the insulin-producing cells under the control of Scer\GAL4^Ilp2.PR partially restores the profile of TAGs and their constituent fatty acids in Itp-r83A^ka1091/Itp-r83A^ug3 mutants to that of the wild type under starvation.

Expression of Ilp2^Scer\UAS.cBa in the insulin-producing cells under the control of Scer\GAL4^Ilp2.PR partially suppresses the overeating seen in itgr[ku] (Itp-r83A^ka1091/Itp-r83A^ug3) mutant females.

(Subramanian et al., 2013)

Co-expression of Ide^Scer\UAS.cTa using Scer\GAL4^sd-SG29.1 significantly suppresses the Ilp2^Scer\UAS.cBa-induced wing-size phenotype.

(Tsuda et al., 2010)

The increased body weight phenotype resulting from the overexpression of Ilp2^Scer\UAS.cBa via Scer\GAL4^arm.PS is dominantly enhanced by ImpL2^Def42.

Overexpression of Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^arm.PS causes lethality in ImpL2^Def42/ImpL2^Def20 mutants.

Co-expression of Ilp2^Scer\UAS.cBa with ImpL2^s.Scer\UAS under the control of Scer\GAL4^ppl.PP suppresses the lethality caused by the overexpression of the individual Ilp2^Scer\UAS.cBa or ImpL2^s.Scer\UAS transgenes.

(Honegger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80936

y¹ w^*; wg^Sp-1/CyO; P{UAS-Ilp2.B}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

IRP^Scer\UAS.cBa

IRP^Scer\UAS.cHa

IRP^Scer\UAS.cIa

Ilp2^Scer\UAS.cBa

Ilp2^Scer\UAS.cIa

Ilp2^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Brogiolo

(Brogiolo et al., 2001)

Saccharomyces cerevisiae UAS construct a of Ikeya

(Ikeya et al., 2002)

Secondary FlyBase IDs

FBal0122091
FBal0141717

References (29)

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