Allele Dmel\Ilp2^Scer\UAS.cBa

General Information
Symbol	Dmel\Ilp2^Scer\UAS.cBa	Species	D. melanogaster
Name		FlyBase ID	FBal0240162
Feature type	allele	Associated gene	Dmel\Ilp2

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms size defective wing
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Brogiolo et al., 2001, Ikeya et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of Ilp2. (Ikeya et al., 2002)
Carried in construct	P{UAS-Ilp2.B} (Brogiolo et al., 2001, Karpac et al., 2009, Ikeya et al., 2002, Wu et al., 2005, Enell et al., 2010, Lee et al., 2008, Honegger et al., 2008, Tsuda et al., 2010, Okamoto et al., 2013)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell number, with Scer\GAL4^hs.PB (Brogiolo et al., 2001) increased cell size, with Scer\GAL4^hs.PB (Brogiolo et al., 2001) large body (Lee et al., 2008) large body, with Scer\GAL4^arm.PS (Honegger et al., 2008) large body, with Scer\GAL4^hs.PB (Brogiolo et al., 2001) lethal, with Scer\GAL4^Act5C.PP (Brogiolo et al., 2001) lethal, with Scer\GAL4^ppl.PP (Honegger et al., 2008) short lived \| nutrition conditional, with Scer\GAL4^Ilp2.PW (Enell et al., 2010) size defective, with Scer\GAL4^sd-SG29.1 (Tsuda et al., 2010) stress response defective, with Scer\GAL4^Ilp2.PR (Karpac et al., 2009) visible, with Scer\GAL4^hs.PB (Brogiolo et al., 2001)
Phenotype Manifest In
	wing, with Scer\GAL4^sd-SG29.1 (Tsuda et al., 2010)
Detailed Description
	Statement Reference Male flies expressing Ilp2[Scer\UAS.cBa] under the control of Scer\GAL4[Ilp2.PW] display a strongly reduced survival at starvation. (Enell et al., 2010) Mis-expression of Ilp2[Scer\UAS.cBa] in developing wing imaginal discs produces adult flies with a wing size larger than that of control. (Tsuda et al., 2010) Flies expressing Ilp2[Scer\UAS.cBa] under the control of Scer\GAL4[Ilp2.PR] show a significantly reduced repression of growth (assayed by measuring wing area) when shifted from 25[o]C to 29[o]C compared to wild-type flies. Flies expressing Ilp2[Scer\UAS.cBa] under the control of Scer\GAL4[Ilp2.PR] show increased sensitivity to oxidative stress sensitivity. (Karpac et al., 2009) Overexpression of Ilp2[Scer\UAS.cBa] under the control of Scer\GAL4[arm.PS] results in increased body size and weight. Overexpression of Ilp2[Scer\UAS.cBa] under the control of Scer\GAL4[ppl.PP] causes lethality. (Honegger et al., 2008) Expression of Ilp2[Scer\UAS.cBa] using Scer\GAL4[Ilp2.PR] increases body size. (Lee et al., 2008) When Ilp2^Scer\UAS.cBa is expressed by under the control of Scer\GAL4^hs.PB, a proportionate increase in body size is seen. Compared to wild-type body weight and number of ommatidia are increased by 39% and 5% respectively. The wing areas is increased by 21% due to significant increases in cell size and number (9% and 11% respectively). (Brogiolo et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Ilp2^Scer\UAS.cBa, Scer\GAL4^arm.PS has large body phenotype, enhanceable by ImpL2[+]/ImpL2^Def42 (Honegger et al., 2008)
Suppressed by
Statement Reference Ilp2^Scer\UAS.cBa, Scer\GAL4^ppl.PP has lethal phenotype, suppressible by ImpL2^s.Scer\UAS, Scer\GAL4^ppl.PP (Honegger et al., 2008) Ilp2^Scer\UAS.cBa, Scer\GAL4^sd-SG29.1 has size defective phenotype, suppressible by Ide^Scer\UAS.cTa, Scer\GAL4^sd-SG29.1 (Tsuda et al., 2010) Ilp2^Scer\UAS.cBa/Ilp2^Scer\UAS.cBa, Scer\GAL4^hs.PB has increased cell number phenotype, suppressible by InR³⁰⁴ (Brogiolo et al., 2001) Ilp2^Scer\UAS.cBa/Ilp2^Scer\UAS.cBa, Scer\GAL4^hs.PB has increased cell size phenotype, suppressible by InR³⁰⁴ (Brogiolo et al., 2001) Ilp2^Scer\UAS.cBa/Ilp2^Scer\UAS.cBa, Scer\GAL4^hs.PB has large body phenotype, suppressible by InR³⁰⁴ (Brogiolo et al., 2001) Ilp2^Scer\UAS.cBa/Ilp2^Scer\UAS.cBa, Scer\GAL4^hs.PB has visible phenotype, suppressible by InR³⁰⁴ (Brogiolo et al., 2001)
Suppressor of
Statement Reference Scer\GAL4^arm.PS/Ilp2^Scer\UAS.cBa is a suppressor of viable phenotype of ImpL2^Def20/ImpL2^Def42 (Honegger et al., 2008)
Phenotype Manifest In
Suppressed by
Statement Reference Ilp2^Scer\UAS.cBa, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible by Ide^Scer\UAS.cTa, Scer\GAL4^sd-SG29.1 (Tsuda et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Ide[Scer\UAS.cTa] using Scer\GAL4[sd-SG29.1] significantly suppresses the Ilp2[Scer\UAS.cBa]-induced wing-size phenotype. (Tsuda et al., 2010) The increased body weight phenotype resulting from the overexpression of Ilp2[Scer\UAS.cBa] via Scer\GAL4[arm.PS] is dominantly enhanced by ImpL2[Def42]. Overexpression of Ilp2[Scer\UAS.cBa] under the control of Scer\GAL4[arm.PS] causes lethality in ImpL2[Def42]/ImpL2[Def20] mutants. Co-expression of Ilp2[Scer\UAS.cBa] with ImpL2[s.Scer\UAS] under the control of Scer\GAL4[ppl.PP] suppresses the lethality caused by the overexpression of the individual Ilp2[Scer\UAS.cBa] or ImpL2[s.Scer\UAS] transgenes. (Honegger et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	Ilp2^Scer\UAS.cBa Ilp2^Scer\UAS.cIa IRP^Scer\UAS.cBa IRP^Scer\UAS.cHa IRP^Scer\UAS.cIa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Brogiolo (Brogiolo et al., 2001) Saccharomyces cerevisiae UAS construct a of Ikeya (Ikeya et al., 2002)
Secondary FlyBase IDs
	FBal0122091 FBal0141717
References ( 9 )
Research paper	Okamoto et al., 2013, Genes Dev. 27(1): 87--97 A secreted decoy of InR antagonizes insulin/IGF signaling to restrict body growth in Drosophila. [FBrf0220509] Enell et al., 2010, PLoS ONE 5(12): e15780 Insulin Signaling, Lifespan and Stress Resistance Are Modulated by Metabotropic GABA Receptors on Insulin Producing Cells in the Brain of Drosophila. [FBrf0212696] Tsuda et al., 2010, FEBS Lett. 584(13): 2916--2920 Insulin-degrading enzyme antagonizes insulin-dependent tissue growth and Abeta-induced neurotoxicity in Drosophila. [FBrf0210992] Karpac et al., 2009, Aging Cell 8(3): 288--295 JNK signaling in insulin-producing cells is required for adaptive responses to stress in Drosophila. [FBrf0208375] Honegger et al., 2008, J. Biol. 7(3): 10 Imp-L2, a putative homolog of vertebrate IGF-binding protein 7, counteracts insulin signaling in Drosophila and is essential for starvation resistance. [FBrf0204716] Lee et al., 2008, Nat. Cell Biol. 10(4): 468--475 Drosophila short neuropeptide F signalling regulates growth by ERK-mediated insulin signalling. [FBrf0204120] Wu et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(37): 13289--13294 Regulation of hunger-driven behaviors by neural ribosomal S6 kinase in Drosophila. [FBrf0188283] Ikeya et al., 2002, Curr. Biol. 12(15): 1293--1300 Nutrient-dependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila. [FBrf0151837] Brogiolo et al., 2001, Curr. Biol. 11(4): 213--221 An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control. [FBrf0134520]

Allele Dmel\Ilp2Scer\UAS.cBa

Allele Dmel\Ilp2^Scer\UAS.cBa