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General Information
Symbol
Dmel\Ilp2UAS.cBa
Species
D. melanogaster
Name
FlyBase ID
FBal0240162
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dilp2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full-length Ilp2 cDNA (GH11579 clone).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Ilp2Scer\UAS.cBa under the control of Scer\GAL4ey.PU has no effect on eye development.

Flies expressing Ilp2Scer\UAS.cBa under the control of Scer\GAL4sNPF-R.2.5 show significantly decreased food consumption compared to controls.

Expression of Ilp2Scer\UAS.cBa in the insulin producing cells (IPCs) under the control of Scer\GAL4Ilp2.PR does not ameliorate either the lethality or developmental delay phenotypes associated with developmental ethanol exposure. Ubiquitous expression under the control of Scer\GAL4arm.PU does suppress the developmental phenotypes. Expression of Ilp2Scer\UAS.cBa under the control of Scer\GAL4Ilp2.PR completely rescues the increased resistance to sedation and ability to develop tolerance seen in wild type larvae raised on 5% ethanol.

Male flies expressing Ilp2Scer\UAS.cBa under the control of Scer\GAL4Ilp2.PW display a strongly reduced survival at starvation.

Mis-expression of Ilp2Scer\UAS.cBa in developing wing imaginal discs produces adult flies with a wing size larger than that of control.

Flies expressing Ilp2Scer\UAS.cBa under the control of Scer\GAL4Ilp2.PR show a significantly reduced repression of growth (assayed by measuring wing area) when shifted from 25[o]C to 29[o]C compared to wild-type flies.

Flies expressing Ilp2Scer\UAS.cBa under the control of Scer\GAL4Ilp2.PR show increased sensitivity to oxidative stress sensitivity.

Overexpression of Ilp2Scer\UAS.cBa under the control of Scer\GAL4arm.PS results in increased body size and weight.

Overexpression of Ilp2Scer\UAS.cBa under the control of Scer\GAL4ppl.PP causes lethality.

Expression of Ilp2Scer\UAS.cBa using Scer\GAL4Ilp2.PR increases body size.

When Ilp2Scer\UAS.cBa is expressed by under the control of Scer\GAL4hs.PB, a proportionate increase in body size is seen. Compared to wild-type body weight and number of ommatidia are increased by 39% and 5% respectively. The wing areas is increased by 21% due to significant increases in cell size and number (9% and 11% respectively).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Ilp2UAS.cBa, Scer\GAL4arm.PS has large body phenotype, enhanceable by ImpL2[+]/ImpL2Def42

Suppressed by
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference

Ilp2UAS.cBa/Scer\GAL4ey.PU is a suppressor of eye | somatic clone | nutrition conditional phenotype of Pten117

NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Ilp2Scer\UAS.cBa under the control of Scer\GAL4ey.PU suppresses the overgrowth found in Pten117 mutant clones under starvation conditions. Furthermore, expression of Ilp2Scer\UAS.cBa restores the growth of both the tissue surrounding the Pten117 clone and peripheral tissue.

Expression of Ilp2Scer\UAS.cBa in the insulin-producing cells under the control of Scer\GAL4Ilp2.PR rescues the starvation resistance and mobilization of TAGs from abdominal fat body cells seen in itgr[ku] (Itp-r83Aka1091/Itp-r83Aug3) mutant flies to wild type levels. The increase in body weight and stored TAG levels are only partially rescued.

Expression of Ilp2Scer\UAS.cBa in the insulin-producing cells under the control of Scer\GAL4Ilp2.PR partially restores the profile of TAGs and their constituent fatty acids in Itp-r83Aka1091/Itp-r83Aug3 mutants to that of the wild type under starvation.

Expression of Ilp2Scer\UAS.cBa in the insulin-producing cells under the control of Scer\GAL4Ilp2.PR partially suppresses the overeating seen in itgr[ku] (Itp-r83Aka1091/Itp-r83Aug3) mutant females.

Co-expression of IdeScer\UAS.cTa using Scer\GAL4sd-SG29.1 significantly suppresses the Ilp2Scer\UAS.cBa-induced wing-size phenotype.

The increased body weight phenotype resulting from the overexpression of Ilp2Scer\UAS.cBa via Scer\GAL4arm.PS is dominantly enhanced by ImpL2Def42.

Overexpression of Ilp2Scer\UAS.cBa under the control of Scer\GAL4arm.PS causes lethality in ImpL2Def42/ImpL2Def20 mutants.

Co-expression of Ilp2Scer\UAS.cBa with ImpL2s.Scer\UAS under the control of Scer\GAL4ppl.PP suppresses the lethality caused by the overexpression of the individual Ilp2Scer\UAS.cBa or ImpL2s.Scer\UAS transgenes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
IRPScer\UAS.cBa
IRPScer\UAS.cHa
IRPScer\UAS.cIa
Ilp2Scer\UAS.cBa
Ilp2Scer\UAS.cIa
Ilp2UAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Brogiolo
Saccharomyces cerevisiae UAS construct a of Ikeya
Secondary FlyBase IDs
  • FBal0122091
  • FBal0141717
References (22)