Open Close
General Information
Symbol
Dmel\cryOUT
Species
D. melanogaster
Name
FlyBase ID
FBal0240222
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion has resulted in a 1490-bp deletion removing the transcription start, exon 1, intron 1, and almost all of exon 2 of cry. The N-terminal 96 amino acids of the total of 542 residues constituting the cry protein is absent in this mutant.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Under dim (35-55 lux) constant light conditions, 88.9% of mutant flies show robust locomotor activity rhythmicity, with an average period length of 24.0 hours (depending on the lines used), whereas control animals are largely arrhythmic under these conditions.

cryOUT mutants subjected to light-dark (LD) (with twilight simulation) and temperature cycles, in phase, display tiny morning peaks and sharp evening peaks of activity. They are completely inactive in the dark phase. After a shift of the LD cycle, cryOUT flies seem to very slowly re-entrain to the LD cycle, with darkness exerting a strong negative masking effect on the activity so that the trajectory of the rhythm is disrupted during the dark phase. Mutant flies take longer to re-synchronize compared to wild-type flies and either seem to free-run (in LD) or to retrain to temperature cycles when in natural light-moonlight cycles (which reduces the negative masking effect of darkness). After a shift of daylight-moonlight (LM) cycles, the evening peak of cryOUT mutants remains at the same phase as before the shift (whereas wild-type follow the shift). cryOUT mutants are mainly entrained by temperature cycles and are neither free-running nor entrained by light-moonlight cycles.

Homozygous cryOUT mutants show short circadian free-running periods under blue light. After experimental exposure to a 300 microT magnetic field, the majority of the cryOUT mutants do not show any period changes, or if changes occur, the changes are significantly smaller than those in wild-type flies.

cryOUT mutant flies show anomalous rhythmic locomotor activity under constant light conditions (wild-type flies are arrhythmic under these conditions).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)