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General Information
Symbol
Dmel\lolaL.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0240224
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the coding region of the 'L' isoform of lola.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

axon & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146

axon & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolae76

dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146

dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolae76

Detailed Description
Statement
Reference

Expression of lolaL.Scer\UAS under the control of Scer\GAL4twi.PG does not affect gonad formation.

The expression of lolaL.Scer\UAS results in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons exhibiting a reduced dendritic tree (expression under the control of Scer\GAL4221) and class IV abdominal dorsal multidendritic ddaC neurons exhibiting a significant decrease in the number of dendrite branch terminals (expression under the control of Scer\GAL4ppk.PG), as compared to controls.

MARCM-mediated expression of lolaL.Scer\UAS, using Scer\GAL4GH146, results in strong targeting defects in antero-dorsal projection neuron (adPN) and lateral (lPN) neuroblast clones. Phenotypes often include a large increase in wandering dendrites in the antennal lobe (AL) and some degree of loss of targeting and ectopic targeting. Often dendrites appear to be largely restricted to one region of the AL, such as dorsal or lateral, and have wandering dendrites through this region in many glomeruli, but fail to innervate normal targets or extend any dendrites into other regions of the AL.

MARCM-mediated expression of lolaL.Scer\UAS, using Scer\GAL4GH146, results in weak phenotypes in vPN neuroblast clones.

The DL1 dendrites are targeted correctly with MARCM-mediated expression of lolaL.Scer\UAS, using Scer\GAL4GH146, but frequently show a large increase in dendritic mass outside of DL1, wandering throughout the AL. In axons, lolaL.Scer\UAS overexpression often results in an increase in branching in mushroom body and lateral horn regions.

MARCM clones simultaneously mutant for lolae76 and lolaL.Scer\UAS, induced using Scer\GAL4GH146, exhibit: i) cell loss and lack of dendritic extensions in adPN and lPN neuroblast clones; ii) a strong reduction of VA1lm and DA1 targeting in vPN clones, with the pan-AL neuron often showing little dendritic process elaboration and only rare innervation of much of the AL.

DL1 MARCM clones simultaneously mutant for lolae76 and lolaL.Scer\UAS, induced using Scer\GAL4GH146 innervate DL1 in addition to having extensive dendritic innervation in the rest of the AL. Axonal defects range from a failure to extend or elaborate branches in the LH to an increase in ectopic branching.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of lolaL.Scer\UAS in the mesoderm under the control of Scer\GAL4twi.PG does not rescue the gonad defects of lolaC28/lola22.05 mutant embryos.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
lolaL.Scer\UAS
lolaL.UAS
Name Synonyms
Secondary FlyBase IDs
    References (3)