FB2025_02 , released April 17, 2025
Allele: Dmel\His2AvHM05177
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General Information
Symbol
Dmel\His2AvHM05177
Species
D. melanogaster
Name
FlyBase ID
FBal0240306
Feature type
allele
Associated gene
Dmel\His2Av
Associated Insertion(s)
Carried in Construct
P{TRiP.HM05177}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Perkins et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Perkins et al., 2009)
Mutagen
in vitro construct
(Perkins et al., 2009)
Progenitor genotype
Carried in construct
P{TRiP.HM05177}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-HM05177
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of His2AvHM05177 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4otu.T:Hsim\VP16 causes significantly decreased female fertility, small ovaries and defects in cyst differentiation in the germarium, as compared to controls.

      Expression of His2AvHM05177 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not affect the development of the germarium or ovariole, but results in a significant proportion of eggs showing the 'dumpless' egg phenotype, as shown by defective late stage egg chambers with short eggs and non-fragmented nurse cells, and a significant reduction in egg-laying, as compared to controls.

      Expression of His2AvHM05177 under the control of Scer\GAL4tj.PU or Scer\GAL4C587 leads to severe packaging defects during oogenesis, ranging from two cysts in a single egg chamber to complete ovariole fusions; many germaria show additional stalk-like structures without germline cysts, and egg laying is nearly completely abolished, as compared to controls.

      (Börner and Becker, 2016)

      Expression of His2AvHM05177 is larval/pupal lethal with melanotic tumors with the Scer\GAL4Act5C.PI driver, results in small rough eyes and ectopic vibrissae with the Scer\GAL4ey.PH driver, mild rough eye phenotype with Scer\GAL4GMR.PF, unfurled wings with Scer\GAL4nub.PK and is pharate pupal lethal at 25[o]C with Scer\GAL4en.PU and leads to occasional vein defects at 18[o]C.

      (Dietz et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      His2AvHM05177
      Name Synonyms
      Secondary FlyBase IDs
        References (8)