Expression of N^JF01637, in combination with a Dicer-2 transgene to enhance RNAi efficiency, under the control of Scer\GAL4^insc-Mz1407 from early first instar stage, in combination with a Gal80[ts] transgene, does not prevent female-specific loss of larval terminal neuroblasts, compared to controls.

Expression of N^JF01637 under the control of Scer\GAL4^Antp-21 in third instar larvae results in a significant decrease in the number of crystal cells in the anterior lymph gland lobes; high levels of reactive oxygen species in cortex and blood cells, but not in the niche; and an increase in the number of lymphocytes in the circulating hemolymph; but does not affect the size or cell number in the niche as compared to controls.

Expression of N^JF01637 under the control of Scer\GAL4^Antp-21 or Scer\GAL4^Hml.Δ in third instar larvae results in an increase in the number of lamellocytes in the anterior lymph gland lobes, most often in the cortical zone, rarely at the medulla/cortex junction, but not in the niche or posterior lobes; and disruption of the basement membrane and dispersion of the anterior lymph gland node, as compared to controls.

Third instar larval lymph gland clones expressing N^JF01637 under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} produce descendents that fail to differentiate into lamellocytes, but cause an increase in cells in the vicinity displaying lamellocyte morphology and cell dispersal.