FlyBase Allele Report: Dmel\Miro[Sd32]

General Information

Symbol

Dmel\Miro^Sd32

Species

D. melanogaster

Name

FlyBase ID

FBal0240382

Feature type

allele

Associated gene

Dmel\Miro

Associated Insertion(s)

Carried in Construct

Also Known As

DMiro^sd32

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Guo et al., 2005)

Mutagen

ethyl methanesulfonate

(Guo et al., 2005)

Progenitor genotype

Cytology

Description

A 29 bp deletion and frame-shifts Miro at Y89, adding 12 abnormal amino acids before terminating.

(Guo et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA with Miro^Sd26

(Tsai et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Iijima-Ando et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage | recessive

(Guo et al., 2005)

abnormal neurophysiology | larval stage (with Miro^B682)

(Guo et al., 2005)

abnormal phototaxis | somatic clone

(Guo et al., 2005)

partially lethal - majority die | larval stage | recessive

(Guo et al., 2005)

partially lethal - majority die | pupal stage | recessive

(Guo et al., 2005)

Phenotype Manifest In

bouton & neuromuscular junction | larval stage (with Miro^B682)

(Guo et al., 2005)

mitochondrion & motor neuron | larval stage (with Miro^B682)

(Russo et al., 2009)

mitochondrion & neuron | larval stage (with Miro^B682)

(Guo et al., 2005)

neuron

(Iijima-Ando et al., 2009)

Detailed Description

Statement

Reference

Homozygous ventral midline precursor cells do not divide more often than wild-type precursors.

(Bossing et al., 2012)

Heterozygous Miro^Sd32 mutants display mitochondrial mislocalisation in neurons.

(Iijima-Ando et al., 2009)

In the motor neurons of Miro^B682/Miro^Sd32 larvae, mitochondria are largely absent from neuro-muscular junctions, and are present in reduced numbers in axons, mainly concentrated close to the ventral ganglion. The proportion of motile mitochondria is significantly reduced in these cells and, unlike in wild-type, many stationary mitochondria are not clustered. In addition, the motile mitochondria (both anterograde and retrograde) are significantly smaller than in wild-type.

(Russo et al., 2009)

Gross eye morphology is normal but phototaxis is defective in flies whose eyes are homozygous for Miro^Sd32.

In neurons of Miro^Sd32/Miro^B682 larvae, mitochondria are retained in the cell bodies and not properly distributed to neuronal processes - most neuromuscular junctions lack mitochondria.

Bouton morphology and distribution is abnormal at neuromuscular junctions in Miro^B682/Miro^Sd32 larvae.

In Miro^Sd32/Miro^B682 larvae, excitatory junction potential evoked by 10Hz stimulation of neuro-muscular junctions (NMJs) starts off normal, but, unlike wild-type, rapidly decays.

(Guo et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Miro^Sd32/Miro[+] is an enhancer of abnormal locomotor behavior phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^elav-C155

(Iijima-Ando et al., 2009)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Miro^Sd32 is a non-suppressor of eye photoreceptor cell | somatic clone phenotype of SdhA¹¹¹⁰

(Mast et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Miro^Sd32 heterozygosity enhances the locomotor defects in flies induced by Scer\GAL4^elav-C155-driven overexpression of Hsap\APP^{Aβ1-42.Scer\UAS.cIa}.

(Iijima-Ando et al., 2009)

Complementation and Rescue Data

Fails to complement

(Guo et al., 2005)

(Guo et al., 2005)

(Guo et al., 2005)

(Guo et al., 2005)

Partially rescued by