Allele Dmel\Miro^Sd32

General Information
Symbol	Dmel\Miro^Sd32	Species	D. melanogaster
Name		FlyBase ID	FBal0240382
Feature type	allele	Associated gene	Dmel\Miro
Also Known As	Miro^sd32

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Guo et al., 2005)
Mutagen	ethyl methanesulfonate (Guo et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A 29 bp deletion and frame-shifts Miro at Y89, adding 12 abnormal amino acids before terminating. (Guo et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective \| larval stage \| recessive (Guo et al., 2005) neurophysiology defective \| larval stage (with Miro^B682) (Guo et al., 2005) partially lethal - majority die \| larval stage \| recessive (Guo et al., 2005) partially lethal - majority die \| pupal stage \| recessive (Guo et al., 2005) phototaxis defective \| somatic clone (Guo et al., 2005)
Phenotype Manifest In
	bouton & neuromuscular junction \| larval stage (with Miro^B682) (Guo et al., 2005) mitochondrion & motor neuron \| larval stage (with Miro^B682) (Russo et al., 2009) mitochondrion & neuron \| larval stage (with Miro^B682) (Guo et al., 2005) neuron (Iijima-Ando et al., 2009)
Detailed Description
	Statement Reference Homozygous ventral midline precursor cells do not divide more often than wild-type precursors. (Bossing et al., 2012) Heterozygous Miro[Sd32] mutants display mitochondrial mislocalisation in neurons. (Iijima-Ando et al., 2009) In the motor neurons of Miro[B682]/Miro[Sd32] larvae, mitochondria are largely absent from neuro-muscular junctions, and are present in reduced numbers in axons, mainly concentrated close to the ventral ganglion. The proportion of motile mitochondria is significantly reduced in these cells and, unlike in wild-type, many stationary mitochondria are not clustered. In addition, the motile mitochondria (both anterograde and retrograde) are significantly smaller than in wild-type. (Russo et al., 2009) Gross eye morphology is normal but phototaxis is defective in flies whose eyes are homozygous for Miro[Sd32]. In neurons of Miro[Sd32]/Miro[B682] larvae, mitochondria are retained in the cell bodies and not properly distributed to neuronal processes - most neuromuscular junctions lack mitochondria. Bouton morphology and distribution is abnormal at neuromuscular junctions in Miro[B682]/Miro[Sd32] larvae. In Miro[Sd32]/Miro[B682] larvae, excitatory junction potential evoked by 10Hz stimulation of neuro-muscular junctions (NMJs) starts off normal, but, unlike wild-type, rapidly decays. (Guo et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Miro^Sd32/Miro[+] is an enhancer of locomotor behavior defective phenotype of Hsap\APP^{Aβ1-42.Scer\UAS.cIa}, Scer\GAL4^elav-C155 (Iijima-Ando et al., 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Miro[Sd32] heterozygosity enhances the locomotor defects in flies induced by Scer\GAL4[elav-C155]-driven overexpression of Hsap\APP[Aβ1-42.Scer\UAS.cIa]. (Iijima-Ando et al., 2009)
Complementation & Rescue Data
Fails to complement	Miro^B682 (Guo et al., 2005) Miro^Sd10 (Guo et al., 2005) Miro^Sd23 (Guo et al., 2005) Miro^Sd26 (Guo et al., 2005)
Partially rescued by	Miro^Sd32, Miro^Sd32, Miro^B682 is partially rescued by Scer\GAL4^Rapgap1-OK6/Miro^{Scer\UAS.T:Hsap\MYC} (Russo et al., 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	dMiro^DS32 (Russo et al., 2009) Miro^Sd32 Miro^sd32 (Mast et al., 2008, ) miro^Sd32 (Iijima-Ando et al., 2009) Miro^SD32 (Bossing et al., 2012)
Name Synonym
Secondary FlyBase IDs
	FBal0239813 FBal0239857
References ( 6 )
Research paper	Bossing et al., 2012, Dev. Cell 23(2): 433--440 Disruption of Microtubule Integrity Initiates Mitosis during CNS Repair. [FBrf0219196] Iijima-Ando et al., 2009, PLoS ONE 4(12): e8310 Mitochondrial mislocalization underlies Abeta42-induced neuronal dysfunction in a Drosophila model of Alzheimer's disease. [FBrf0209556] Russo et al., 2009, J. Neurosci. 29(17): 5443--5455 Drosophila Miro is required for both anterograde and retrograde axonal mitochondrial transport. [FBrf0207789] Mast et al., 2008, Development 135(15): 2669--2679 Reactive oxygen species act remotely to cause synapse loss in a Drosophila model of developmental mitochondrial encephalopathy. [FBrf0207179] Guo et al., 2005, Neuron 47(3): 379--393 The GTPase dMiro is required for axonal transport of mitochondria to Drosophila synapses. [FBrf0188178]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Allele Dmel\MiroSd32

Allele Dmel\Miro^Sd32