Allele Dmel\TrpA1¹

General Information
Symbol	Dmel\TrpA1¹	Species	D. melanogaster
Name		FlyBase ID	FBal0240383
Feature type	allele	Associated gene	Dmel\TrpA1
Also Known As	trpA1¹

Allele class
Mutagen	recombination
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	recombination (Kwon et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	taste perception defective \| recessive (Kim et al., 2010, Kohno et al., 2010) thermotaxis behavior defective (Kwon et al., 2008) thermotaxis behavior defective \| larval stage (Shen et al., 2011) thermotaxis behavior defective \| third instar larval stage (Kwon et al., 2010)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant larvae show defects in discrimination between 18[o]C and 24[o]C in a binary choice thermotaxis assay compared to controls. Mutant larvae show reduced turning behaviour compared to control larvae when they move into a 24[o]C zone from an 18[o]C zone (turning behaviour when they move from an 18[o]C zone into a 24[o]C zone is similar in both mutant and wild-type larvae). (Shen et al., 2011) TrpA1[1] flies exhibit the wild type preference for 5mM sucrose over 1mM sucrose and display wild type avoidance of most bitter chemicals tested in food choice assays. However, mutants display a reduced aversion to aristolochic acid compared to wild type. TrpA1[1] flies show a similar frequency of action potentials to wild type in response to sucrose, caffeine and most other bitter compounds in tip recordings of sensillum s6. However, in contrast to wild type, mutants have virtually no aristolochic acid-induced action potentials. (Kim et al., 2010) Gustatory responses of wild-type but not TrpA1[1] flies to sucrose are suppressed by allyl isothiocyanate. (Kohno et al., 2010) Mutant third instar larvae show the same preference for 17.5[o]C over 14[o]C in a two-way choice test as do wild-type controls. The normal preference of third instar larvae for 17.5[o]C over either 22[o] or 24[o]C in a two-way choice test is eliminated in mutant larvae. (Kwon et al., 2010) TrpA1[1] mutants do not exhibit thermotaxis (i.e. a preference to move across a temperature gradient). The ability of TrpA1[1] mutants to chose 18 over 19, 20 or 22[o]c is also eliminated. However, TrpA1[1] larvae do discern 18[o]C from 14-16[o]c. similarly to wild-type, and they prefer 18[o]C over 26-32[o]C, but to a lesser extent than wild-type. (Kwon et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference TrpA1¹ has taste perception defective \| recessive phenotype, suppressible \| partially by Scer\GAL4^TrpA1.PR/Amel\HsTRPA^Scer\UAS.cKa (Kohno et al., 2010)
Suppressor of
Statement Reference TrpA1¹/Scer\GAL4^TrpA1.PR is a suppressor of thermotaxis behavior defective phenotype of Scer\GAL4^TrpA1.PR, norpA^Scer\UAS.ckb (Kwon et al., 2008)
NOT Suppressor of
Statement Reference TrpA1¹ is a non-suppressor of thermotaxis behavior defective phenotype of Gαq¹ (Kwon et al., 2008)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Introduction of TrpA1[1] into a Gα49B[1] background results in loss of temperature selection identical to that resulting from the Gα49B[1] alone. The thermotactic preference for 18[o]C in norpA[Scer\UAS.ckb]; Scer\GAL4[TrpA1.PR] larvae is suppressed in a TrpA1[1] heterozygous background. (Kwon et al., 2008)
Xenogenetic Interactions
Statement Reference Expression of Amel\HsTRPA[Scer\UAS.cKa] under the control of Scer\GAL4[TrpA1.PR] restores the avoidance response of TrpA1[1] mutant flies to allyl isothiocyanate. Expression of Amel\HsTRPA[Scer\UAS.cKa] under the control of Scer\GAL4[TrpA1.PR] fails to restore the aversive response of TrpA1[1] mutant flies to aristolochic acid. (Kohno et al., 2010)
Complementation & Rescue Data
Rescued by	TrpA1¹ is rescued by TrpA1^Scer\UAS.cKa/Scer\GAL4^Gr66a.PD (Kim et al., 2010) TrpA1¹ is rescued by TrpA1^Scer\UAS.cKb/Scer\GAL4^TrpA1.PR (Kwon et al., 2008) TrpA1¹ is rescued by TrpA1^Scer\UAS.cKc/Scer\GAL4^TrpA1.PR (Kohno et al., 2010)
Comments	Expression of TrpA1[Scer\UAS.cKa] using Scer\GAL4[Gr66a.PD] rescues the loss of avoidance to aristolochic acid shown by TrpA1[1] mutants. Expression of TrpA1[Scer\UAS.cKa] using Scer\GAL4[Gr66a.PD] rescues the loss of aristolochic acid-induced action potentials shown by TrpA1[1] flies in tip recordings of sensillum s6. (Kim et al., 2010) Expression of TrpA1[Scer\UAS.cKc] under the control of Scer\GAL4[TrpA1.PR] restores the aversive response of TrpA1[1] mutant flies to allyl isothiocyanate. (Kohno et al., 2010) The TrpA1[1] thermotactic phenotype is rescued by expression of TrpA1[Scer\UAS.cKb] under the control of Scer\GAL4[TrpA1.PR]. (Kwon et al., 2008)
Stocks ( 9 )
Bloomington	36342 TrpA1¹ 26504 w¹¹¹⁸; TrpA1¹ 36922 w^; TrpA1¹, P{TrpA1-GAL4.R}3 36925 w^; TrpA1¹ P{UAS-TrpA1.RC}attP2 36927 w^; TrpA1¹ P{UAS-TrpA1.RD}attP2 36926 w^; TrpA1¹ P{UAS-DmojTRPA1.S}attP2 36929 w^; TrpA1¹ P{UAS-DmojTRPA1.RC}attP2 36930 w^; TrpA1¹ P{UAS-DmojTRPA1.RD}attP2 36924 w^*; TrpA1¹ P{UAS-AmHsTRPA.attB}attP2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	TrpA1¹ trpA1¹ (Kwon et al., 2008, Kwon et al., 2010, Shen et al., 2011, Kim et al., 2010, Kohno et al., 2010) trpA¹ (Kwon et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Shen et al., 2011, Science 331(6022): 1333--1336 Function of rhodopsin in temperature discrimination in Drosophila. [FBrf0213227] Kim et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(18): 8440--8445 Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons. [FBrf0210738] Kohno et al., 2010, J. Neurosci. 30(37): 12219--12229 Honey bee thermal/chemical sensor, AmHsTRPA, reveals neofunctionalization and loss of transient receptor potential channel genes. [FBrf0216546] Kwon et al., 2010, J. Neurosci. 30(31): 10465--10471 Fine Thermotactic Discrimination between the Optimal and Slightly Cooler Temperatures via a TRPV Channel in Chordotonal Neurons. [FBrf0211446] Kwon et al., 2008, Nat. Neurosci. 11(8): 871--873 Control of thermotactic behavior via coupling of a TRP channel to a phospholipase C signaling cascade. [FBrf0205753]

Allele Dmel\TrpA11

Allele Dmel\TrpA1¹