|Feature type||allele||Associated gene||Dmel\TrpA1|
|Also Known As||trpA11|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
Mutant larvae show defects in discrimination between 18[o]C and 24[o]C in a binary choice thermotaxis assay compared to controls. Mutant larvae show reduced turning behaviour compared to control larvae when they move into a 24[o]C zone from an 18[o]C zone (turning behaviour when they move from an 18[o]C zone into a 24[o]C zone is similar in both mutant and wild-type larvae).
TrpA1 flies exhibit the wild type preference for 5mM sucrose over 1mM sucrose and display wild type avoidance of most bitter chemicals tested in food choice assays. However, mutants display a reduced aversion to aristolochic acid compared to wild type. TrpA1 flies show a similar frequency of action potentials to wild type in response to sucrose, caffeine and most other bitter compounds in tip recordings of sensillum s6. However, in contrast to wild type, mutants have virtually no aristolochic acid-induced action potentials.
Gustatory responses of wild-type but not TrpA1 flies to sucrose are suppressed by allyl isothiocyanate.
Mutant third instar larvae show the same preference for 17.5[o]C over 14[o]C in a two-way choice test as do wild-type controls. The normal preference of third instar larvae for 17.5[o]C over either 22[o] or 24[o]C in a two-way choice test is eliminated in mutant larvae.
TrpA1 mutants do not exhibit thermotaxis (i.e. a preference to move across a temperature gradient). The ability of TrpA1 mutants to chose 18 over 19, 20 or 22[o]c is also eliminated. However, TrpA1 larvae do discern 18[o]C from 14-16[o]c. similarly to wild-type, and they prefer 18[o]C over 26-32[o]C, but to a lesser extent than wild-type.
|NOT Suppressor of|
|Phenotype Manifest In|
Introduction of TrpA1 into a Gα49B background results in loss of temperature selection identical to that resulting from the Gα49B alone. The thermotactic preference for 18[o]C in norpA[Scer\UAS.ckb]; Scer\GAL4[TrpA1.PR] larvae is suppressed in a TrpA1 heterozygous background.
Expression of Amel\HsTRPA[Scer\UAS.cKa] under the control of Scer\GAL4[TrpA1.PR] restores the avoidance response of TrpA1 mutant flies to allyl isothiocyanate. Expression of Amel\HsTRPA[Scer\UAS.cKa] under the control of Scer\GAL4[TrpA1.PR] fails to restore the aversive response of TrpA1 mutant flies to aristolochic acid.
|Complementation & Rescue Data|
Expression of TrpA1[Scer\UAS.cKa] using Scer\GAL4[Gr66a.PD] rescues the loss of avoidance to aristolochic acid shown by TrpA1 mutants. Expression of TrpA1[Scer\UAS.cKa] using Scer\GAL4[Gr66a.PD] rescues the loss of aristolochic acid-induced action potentials shown by TrpA1 flies in tip recordings of sensillum s6.
Expression of TrpA1[Scer\UAS.cKc] under the control of Scer\GAL4[TrpA1.PR] restores the aversive response of TrpA1 mutant flies to allyl isothiocyanate.
|Stocks ( 9 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 5 )|