FB2025_01 , released February 20, 2025
Allele: Dmel\tkvRNAi.IR1.UAS
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General Information
Symbol
Dmel\tkvRNAi.IR1.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0240398
Feature type
allele
Associated gene
Dmel\tkv
Associated Insertion(s)
Carried in Construct
P{UAS-tkv.RNAi.IR1}
Key Links
Transgenic product class
RNAi_reagent
(Cordero et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Cordero et al., 2007)
Mutagen
in vitro construct
(Cordero et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-tkv.RNAi.IR1}
Cytology
Description

UAS sequences drive expression of an inverted repeat which targets unspecified sequence in the 5' half of the tkv mRNA.

(Cordero et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
tkv
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In pupal retinas tkvIR1.Scer\UAS; tkvIR2.Scer\UAS; Scer\GAL4GMR.PF at around 25 hours APF inter-ommatidial precursor cells fail to maintain stable, adherens junction mediated contacts with each, allowing abberant contacts to form between primary pigment cells in adjacent ommatidia. The affected inter-ommatidial precursor cells often fail to maintain a normal apical profile.

      (Cordero et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The frequency of inter-ommatidial pattering defects in tkvIR1.Scer\UAS; tkvIR2.Scer\UAS; Scer\GAL4GMR.PF pupal retinas at 42 hours APF is significantly enhanced by Rho172F/+. This enhancement is not seen with Cdc42unspecified/+, Rac1unspecified/+, Rac2unspecified/+, or Mtlunspecified/+.

      (Cordero et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      dsRNA expressed from this construct used to study the phenotypic consequences of depleting tkv mRNA targeted using a Scer\GAL4 driver.

      (Cordero et al., 2007)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      tkvIR1.Scer\UAS
      tkvRNAi.IR1.UAS
      tkvdsRNA.IR1.Scer\UAS
      tkvdsRNA.IR1.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)