FlyBase Allele Report: Dmel\tkv[RNAi.IR2.UAS]

General Information

Symbol

Dmel\tkv^RNAi.IR2.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0240399

Feature type

allele

Associated gene

Dmel\tkv

Associated Insertion(s)

Carried in Construct

P{UAS-tkv.RNAi.IR2}

Key Links

Transgenic product class

RNAi_reagent

(Cordero et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Cordero et al., 2007)

Mutagen

in vitro construct

(Cordero et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-tkv.RNAi.IR2}

Cytology

Description

UAS sequences drive expression of an inverted repeat which targets unspecified sequence in the 5' half of the tkv mRNA.

(Cordero et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tkv

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Scer\GAL4^sd-SG29.1

(Seppa et al., 2008)

Phenotype Manifest In

adherens junction (with tkv^RNAi.IR1.UAS), with Scer\GAL4^GMR.PF

(Cordero et al., 2007)

interommatidial precursor cell | pupal stage P6 (with tkv^RNAi.IR1.UAS), with Scer\GAL4^GMR.PF

(Cordero et al., 2007)

retina | pupal stage P6, with Scer\GAL4^GMR.PF, tkv^RNAi.IR1.UAS

(Cordero et al., 2007)

wing vein | ectopic, with Scer\GAL4^sd-SG29.1

(Seppa et al., 2008)

wing vein L2 | distal, with Scer\GAL4^sd-SG29.1

(Cordero et al., 2007)

wing vein L5 | distal, with Scer\GAL4^sd-SG29.1

(Cordero et al., 2007)

Detailed Description

Statement

Reference

Expression of tkv^{dsRNA.IR2.Scer\UAS} under the control of Scer\GAL4^sd-SG29.1 results in mild expansion of wing vein material.

(Seppa et al., 2008)

In pupal retinas tkv^IR1.Scer\UAS; tkv^IR2.Scer\UAS; Scer\GAL4^GMR.PF at around 25 hours APF inter-ommatidial precursor cells fail to maintain stable, adherens junction mediated contacts with each, allowing abberant contacts to form between primary pigment cells in adjacent ommatidia. The affected inter-ommatidial precursor cells often fail to maintain a normal apical profile.

In tkv^IR2.Scer\UAS; Scer\GAL4^sd-SG29.1 adults, have thickened wing veins - with distal regions of L2 and L5 most severely affected..

(Cordero et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^sd-SG29.1/pyd^RNAi.3.UAS

(Seppa et al., 2008)

Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS has visible | adult stage phenotype, enhanceable by pyd^tex2/pyd[+]

(Seppa et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS has wing vein | ectopic phenotype, enhanceable by Scer\GAL4^sd-SG29.1/pyd^RNAi.3.UAS

(Seppa et al., 2008)

Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS has wing vein | ectopic phenotype, enhanceable by pyd^tex2/pyd[+]

(Seppa et al., 2008)

Scer\GAL4^GMR.PF, tkv^RNAi.IR1.UAS, tkv^RNAi.IR2.UAS has retina | pupal stage P7 phenotype, enhanceable by Rho1^72F/Rho1[+]

(Cordero et al., 2007)

Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS has wing vein phenotype, enhanceable by Rho1^72F/Rho1[+]

(Cordero et al., 2007)

Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS has wing vein phenotype, enhanceable by shg[+]/shg^R69

(Cordero et al., 2007)

Scer\GAL4^GMR.PF, tkv^RNAi.IR2.UAS/tkv^RNAi.IR1.UAS has interommatidial precursor cell | pupal stage P7 phenotype, enhanceable by Rho1^72F/Rho1[+]

(Cordero et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of pyd^{3.dsRNA.Scer\UAS} strongly enhances the wing vein defects seen when tkv^{dsRNA.IR2.Scer\UAS} is expressed under the control of Scer\GAL4^sd-SG29.1.

One copy of pyd^tex2 strongly enhances the wing vein defects seen when tkv^{dsRNA.IR2.Scer\UAS} is expressed under the control of Scer\GAL4^sd-SG29.1.

(Seppa et al., 2008)

The frequency of inter-ommatidial pattering defects in tkv^IR1.Scer\UAS; tkv^IR2.Scer\UAS; Scer\GAL4^GMR.PF pupal retinas at 42 hours APF is significantly enhanced by Rho1^72F/+. This enhancement is not seen with Cdc42^unspecified/+, Rac1^unspecified/+, Rac2^unspecified/+, or Mtl^unspecified/+.

The wing vein thickening phenotype seen in tkv^IR2.Scer\UAS; Scer\GAL4^sd-SG29.1 adults is enhanced by Rho1^72F/+ or shg^R69/+, but not by Cdc42^unspecified/+, Rac1^unspecified/+, Rac2^unspecified/+, or Mtl^unspecified/+.

(Cordero et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

dsRNA expressed from this construct used to study the phenotypic consequences of depleting tkv mRNA targeted using a Scer\GAL4 driver.

(Cordero et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

tkv^IR2.Scer\UAS

tkv^RNAi.IR2.UAS

tkv^{dsRNA.IR2.Scer\UAS}

tkv^{dsRNA.IR2.UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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