FB2025_01 , released February 20, 2025
Allele: Dmel\HipkUAS.Tag:HA
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General Information
Symbol
Dmel\HipkUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0240413
Feature type
allele
Associated gene
Dmel\Hipk
Associated Insertion(s)
Carried in Construct
P{UAS-Hipk.HA}
Also Known As
UAs-hipk, UAS-HA-hipk, UAS-hipk3M
Key Links
Transgenic product class
cDNA
(Lee et al., 2009)
wild_type
(Lee et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lee et al., 2009)
wild_type
(Lee et al., 2009)
Mutagen
in vitro construct
(Lee et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Hipk.HA}
Cytology
Description

UASt regulatory sequences drive expression of full length Hipk cDNA which is tagged with Tag:HA.

(Lee et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hipk
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Blaquiere et al., 2018)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  amyotrophic lateral sclerosis type 10
      modeled by TBPHUAS.cUa
      (Wang et al., 2020)
      model of  cancer
      is NOT ameliorated by DeltaDN.UAS
      (Blaquiere et al., 2018)
      is NOT ameliorated by AxnUAS.GFP
      (Blaquiere et al., 2018)
      is NOT ameliorated by DeltaDN.UAS
      (Blaquiere et al., 2018)
      is NOT ameliorated by EgfrDN.cUa.UAS
      (Blaquiere et al., 2018)
      is exacerbated by NICN.UAS
      (Blaquiere et al., 2018)
      is exacerbated by Ras85DV12.UAS
      (Blaquiere et al., 2018)
      is exacerbated by Stat92EUAS.cUa
      (Blaquiere et al., 2018)
      is exacerbated by armS10.UAS.Tag:MYC
      (Blaquiere et al., 2018)
      is NOT ameliorated by bskDN.UAS
      (Blaquiere et al., 2018)
      is exacerbated by ci5m.UAS
      (Blaquiere et al., 2018)
      is NOT ameliorated by ci76.UAS
      (Blaquiere et al., 2018)
      is exacerbated by egrUAS.cUa
      (Blaquiere et al., 2018)
      is NOT ameliorated by hopKK105136
      (Blaquiere et al., 2018)
      is NOT ameliorated by panGD1506
      (Blaquiere et al., 2018)
      is NOT ameliorated by upd3VDRC.cUa
      (Blaquiere et al., 2018)
      is NOT ameliorated by ykiHMS00041
      (Blaquiere et al., 2018)
      is exacerbated by ykiS168A.UAS.EGFP,Tag:HA
      (Blaquiere et al., 2018)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of HipkUAS.Tag:HA under the control of Scer\GAL4dpp.blk1 leads to overproliferation and cell spreading of Hipk[UAS. Tag:HA ] expressing cells in third instar larval wing discs compared to controls.

      (Tettweiler et al., 2019)

      The expression of HipkUAS.Tag:HA under the control of Scer\GAL4dpp.blk1 at 25[o]C induces dramatic overgrowth of eye, wing and leg imaginal discs, characterized by expansion of the Dpp expression domain and tissue folds and protrusions; at 29[o]C, this expression induces overgrown wing discs that show apoptosis (cleaved Caspase 3 staining) within the Dpp expression domain. Unlike controls, a) clusters of pigmented retinal cells are occasionally found in the adult thorax, b) eye disc cells extrude from the disc and continue to proliferate after leaving the disc, c) cells originating from the wing disc's Dpp-positive domain are found towards both anterior and posterior parts of disc, d) wing disc cells appear to intercalate into the basement membrane and extrude through holes in the basement membrane.

      Expression of HipkUAS.Tag:HA under the control of Scer\GAL4dpp.blk1 and Scer\GAL4Hml.Δ induces melanotic tumors in larvae and adults, respectively.

      The expression of HipkUAS.Tag:HA under the control of Scer\GAL4ey.PH occasionally leads to a cluster of pigmented retinal cells (from the ey domain in the eye) being observed in the adult thorax, despite of no obvious eye defects. Unlike control eye discs, which show a tight boundary of photoreceptor cells at the posterior margin, this expression alters cell morphology, with jagged cell extensions that protrude towards the posterior margin of the disc.

      Clonal expression of HipkUAS.Tag:HA under the control of Scer\GAL4Act5C.PP in the wing disc induces apoptosis (cleaved Caspase 3 staining) in a cell autonomous manner.

      (Blaquiere et al., 2018)

      Expression of hipkScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4bi.PU results in dosage-dependent overgrowth in third instar larval wing discs.

      (Chen and Verheyen, 2012)

      Expression of hipkScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PH at 29[o]C results in rough eyes, of which 33% also display cuticle-like structures. 39% of these flies have overgrown eyes and overgrowths of eye discs are also seen in third instar larvae.

      (Lee et al., 2009)

      Expression of one copy of hipkScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4bi.PU results in the formation of an additional wing margin and outgrowths from the ventral side of the wing. Expression of two copies of hipkScer\UAS.T:Ivir\HA1 results in the formation of additional wing tissue outgrowths.

      Ectopic expression of hipkScer\UAS.T:Ivir\HA1 in the wing using Scer\GAL469B results in ectopic vein formation.

      (Lee et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by DeltaDN.UAS, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by hopKK105136, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by upd3VDRC.cUa, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by armS10.UAS.Tag:MYC, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by Stat92EUAS.cUa, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible | partially by egrUAS.cUa, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by ykiS168A.UAS.EGFP,Tag:HA, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by NICN.UAS, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by ci5m.UAS, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has neoplasia phenotype, non-suppressible by EgfrDN.cUa.UAS, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has melanotic mass phenotype | larval stage phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has visible | larval stage phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has neoplasia phenotype, non-suppressible by DeltaDN.UAS, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has neoplasia phenotype, non-suppressible by hopKK105136, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by panGD1506, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by AxnUAS.GFP, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by EgfrDN.cUa.UAS, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by bskDN.UAS, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)

      HipkUAS.Tag:HA, Scer\GAL4dpp.blk1 has abnormal cell migration | larval stage phenotype, non-suppressible by ykiHMS00041, Scer\GAL4dpp.blk1

      (Blaquiere et al., 2018)
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of hipkScer\UAS.T:Ivir\HA1 and ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4hh-Gal4 enhances the wing disc overgrowth seen when either construct is expressed alone.

      Expression of hipkScer\UAS.T:Ivir\HA1 suppresses the overgrowth seen when ykiS168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 is expressed under the control of Scer\GAL4ptc-559.1. The phenotype is rescued in all tissues examined, including the wing disc and the eye. Fewer ykiS168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 expressing cells are seen, indicating that cell proliferation has been reduced.

      (Chen and Verheyen, 2012)

      The eye overproliferation defect caused by expression of DlScer\UAS.cDa under the control of Scer\GAL4ey.PH is enhanced by co-expression of hipkScer\UAS.T:Ivir\HA1.

      The eye loss phenotype caused by expression of NECN.Scer\UAS.cGa under the control of Scer\GAL4ey.PH is partially suppressed by co-expression of hipkScer\UAS.T:Ivir\HA1.

      Co-expression of hipkScer\UAS.T:Ivir\HA1 partially suppresses the eye loss phenotype caused by expression of groScer\UAS.cAa under the control of Scer\GAL4ey.PH.

      Co-expression of hipkScer\UAS.T:Ivir\HA1 does not suppress the eye loss phenotype caused by expression of groAA.cLa.Scer\UAS under the control of Scer\GAL4ey.PH.

      Co-expression of hipkScer\UAS.T:Ivir\HA1 partially suppresses the complete eye loss caused by expression of HScer\UAS.cGa under the control of Scer\GAL4ey.PH; a small eye is seen.

      Co-expression of hipkScer\UAS.T:Ivir\HA1 partially rescues the small eye phenotype caused by expression of fngScer\UAS.cKa under the control of Scer\GAL4ey.PH.

      (Lee et al., 2009)

      Expression of hipkScer\UAS.T:Ivir\HA1 under the control of Scer\GAL469B in a wg1/wgl-17 mutant background results in proper wing development and rescues the wing-to-notum transformation.

      Expression of hipkScer\UAS.T:Ivir\HA1 in animals expressing fz2Scer\UAS.N under the control of Scer\GAL469B rescues the severely notched wing phenotype.

      (Lee et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference

      Individuals co-expressing BacA\p35UAS.cHa and HipkUAS.Tag:HA under the control of Scer\GAL4dpp.blk1 arrest at the third larval stage for an extended period of time (beyond 10 days) and eventually die as larvae. BacA\p35UAS.cHa co-expression enhances the wing disc tumorigenesis of the expression of HipkUAS.Tag:HA expression (the Dpp expression domain cells expand substantially and occupy almost the entire disc) and does not suppress the melanotic masses.

      (Blaquiere et al., 2018)
      Complementation and Rescue Data
      Rescued by

      HipkUAS.Tag:HA is rescued by HipkKK107857

      (Blaquiere et al., 2018)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      HipkScer\UAS.T:Ivir\HA1
      HipkUAS.Tag:HA
      hipkScer\UAS.T:Ivir\HA1
      Name Synonyms
      Secondary FlyBase IDs
        References (12)