Allele Dmel\aux^G257E

General Information
Symbol	Dmel\aux^G257E	Species	D. melanogaster
Name		FlyBase ID	FBal0240440
Feature type	allele	Associated gene	Dmel\aux
Also Known As	dAux^G257E

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Kandachar et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:48,203..48,203 na_change=G48203A pr_change=G257E \| aux-PA; G257E \| aux-PB; G257E \| aux-PC; G249E \| aux-PD reported_pr_change=G257E comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. (Kandachar et al., 2008)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G257E. (Kandachar et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	male sterile (with aux^I670K) (Zhou et al., 2011) visible (Cheli et al., 2010)
Phenotype Manifest In
	cystic bulge (with aux^I670K) (Zhou et al., 2011) eye (Cheli et al., 2010) eye (with aux^I670K) (Zhou et al., 2011) eye photoreceptor cell \| supernumerary (with aux^I670K) (Zhou et al., 2011) individualization stage spermatid (with aux^I670K) (Zhou et al., 2011) male germline stem cell (with aux^I670K) (Zhou et al., 2011) scutellar bristle \| supernumerary (with aux^I670K) (Zhou et al., 2011) sternopleural bristle \| supernumerary (with aux^I670K) (Zhou et al., 2011)
Detailed Description
	Statement Reference aux[I670K]/aux[G257E] adults exhibit rough eyes, with extra photoreceptor cells and occasional extra bristles on the notum, sternopleurum, and scutellum. aux[I670K]/aux[G257E] mutant males, when mated to wild-type females, do not produce any embryos that hatch. Spermatids do develop in the testis, however the seminal vesicles are empty in adult male flies. The morphology of the testis hub cells appear normal in these animals. A slightly reduced number of germ cells is observed compared to wild-type testes. Cytokinesis is mostly normal in these animals. In 5% of spermatids examined, an enlarged mitochondrial derivative associated with two nuclei is observed. Spermatid individualisation is disrupted in mutants, and migration of the investment cone along spermatid cysts does not occur normally. (Zhou et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	aux^{FL.βTub85D.T:Disc\RFP-mRFP}/aux^G257E partially rescues aux^I670K (Zhou et al., 2011) aux^G257E/aux^{CJ.βTub85D.T:Disc\RFP-mRFP} partially rescues aux^I670K (Zhou et al., 2011) aux^{ΔK.βTub85D.T:Disc\RFP-mRFP}/aux^G257E partially rescues aux^I670K (Zhou et al., 2011)
Fails to rescue	aux^{ΔC.βTub85D.T:Disc\RFP-mRFP}/aux^G257E fails to rescue aux^I670K (Zhou et al., 2011) aux^{ΔJ.βTub85D.T:Disc\RFP-mRFP}/aux^G257E fails to rescue aux^I670K (Zhou et al., 2011)
Comments	Expression of aux[FL.βTub85D.T:Disc\RFP-mRFP] in aux[I670K]/aux[G257E] mutants rescues male sterility. The rough eye and supernumerary bristle phenotypes are not rescued. Expression of aux[ΔK.βTub85D.T:Disc\RFP-mRFP] in aux[I670K]/aux[G257E] mutants rescues male sterility. Expression of aux[CJ.βTub85D.T:Disc\RFP-mRFP] in aux[I670K]/aux[G257E] mutants rescues male sterility. Expression of aux[ΔJ.βTub85D.T:Disc\RFP-mRFP] in aux[I670K]/aux[G257E] mutants fails to rescue male sterility. Expression of aux[ΔC.βTub85D.T:Disc\RFP-mRFP] in aux[I670K]/aux[G257E] mutants fails to rescue male sterility. (Zhou et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	aux^G257E (Cheli et al., 2010) dAux^G257E (Kandachar et al., 2008, Zhou et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Zhou et al., 2011, Development 138(6): 1111--1120 Auxilin is required for formation of Golgi-derived clathrin-coated vesicles during Drosophila spermatogenesis. [FBrf0213068] Cheli et al., 2010, Hum. Mol. Genet. 19(5): 861--878 Genetic modifiers of abnormal organelle biogenesis in a Drosophila model of BLOC-1 deficiency. [FBrf0209903] Kandachar et al., 2008, BMC Dev. Biol. 8: 50 The clathrin-binding motif and the J-domain of Drosophila Auxilin are essential for facilitating Notch ligand endocytosis. [FBrf0205482]

Allele Dmel\auxG257E

Allele Dmel\aux^G257E