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General Information
Symbol
Dmel\pashaKO
Species
D. melanogaster
Name
FlyBase ID
FBal0240501
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    deletion
    Comment:

    Inferred breakpoints of ~9.2 kb deletion generated by FLP-mediated recombination that removes the entire pasha gene and the first exon of kek6. A hybrid piggyBac w[+] insertion remains at the pasha locus.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Cytology
    Nature of the lesion
    Statement
    Reference

    Recombination between the two progenitor insertions has resulted in a deletion of the sequence between them, producing a deletion of approximately 10kb that includes the pasha gene. A hybrid piggyBac w[+] insertion remains at the pasha locus.

    Insertion components
    PBac{RB3.WH3}pashaKO
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Homozygous pashaKO mutants die as malformed pupae with severely reduced central nervous systems.

    Homozygous pashaKO adult mushroom body clones display a reduction in cell number and absence of α'/β' and α/β neurons.

    pashaKO mutant mushroom body clones display γ neuron disorganisation and mistargeting, as evidenced by inappropriate extension across the midline.

    pashaKO adPN clones contain fewer cells than in wild-type cells - approximately 45.8 compared to 59 cells. This reduction is most likely due to premature adPN neuroblasts exit cell cycle during mid- to late larval stages, as cell number defects are not found in Scer\GAL4GH146.PB>Avic\GFPS65T.Ubi-p63E.T:SV40\nls2-labelled pashaKO clones.

    Scer\GAL4acj6-PG63>Avic\GFPS65T.Ubi-p63E.T:SV40\nls2-labelled pashaKO clones display dendritic mistargeting, as indicated by spillover of dendritic branches into incorrect glomerular classes.

    Mutant third instar larvae usually lack any obvious imaginal discs.

    Homozygotes die as late third instar larvae. Homozygous larvae essentially lack imaginal discs, with only rudiments remaining. The optic lobes are also strongly reduced in size, although the brain stem appear fairly normal.

    Females containing homozygous germline clones are unable to lay eggs.

    Homozygous clones result in wing blistering, external loss of notum bristles and small, rough eyes.

    Random homozygous clones induced in the imaginal discs are smaller than their wild-type twin spots.

    Homozygous clones contribute far less to the adult eye than their wild-type twin spots.

    Homozygous clones induced in the eye disc are unable to eliminate Minute heterozygous

    cells, in contrast to wild-type clones.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (6)