Homozygous pashaKO mutants die as malformed pupae with severely reduced central nervous systems.
Homozygous pashaKO adult mushroom body clones display a reduction in cell number and absence of α'/β' and α/β neurons.
pashaKO mutant mushroom body clones display γ neuron disorganisation and mistargeting, as evidenced by inappropriate extension across the midline.
pashaKO adPN clones contain fewer cells than in wild-type cells - approximately 45.8 compared to 59 cells. This reduction is most likely due to premature adPN neuroblasts exit cell cycle during mid- to late larval stages, as cell number defects are not found in Scer\GAL4GH146.PB>Avic\GFPS65T.Ubi-p63E.T:SV40\nls2-labelled pashaKO clones.
Scer\GAL4acj6-PG63>Avic\GFPS65T.Ubi-p63E.T:SV40\nls2-labelled pashaKO clones display dendritic mistargeting, as indicated by spillover of dendritic branches into incorrect glomerular classes.
Mutant third instar larvae usually lack any obvious imaginal discs.
Homozygotes die as late third instar larvae. Homozygous larvae essentially lack imaginal discs, with only rudiments remaining. The optic lobes are also strongly reduced in size, although the brain stem appear fairly normal.
Females containing homozygous germline clones are unable to lay eggs.
Homozygous clones result in wing blistering, external loss of notum bristles and small, rough eyes.
Random homozygous clones induced in the imaginal discs are smaller than their wild-type twin spots.
Homozygous clones contribute far less to the adult eye than their wild-type twin spots.
Homozygous clones induced in the eye disc are unable to eliminate Minute heterozygous
cells, in contrast to wild-type clones.