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General Information
Symbol
Dmel\magutetO.cLa
Species
D. melanogaster
Name
Tn10 tetracycline operator construct a of Li
FlyBase ID
FBal0240539
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

magu cDNA is cloned downstream from tetO sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Five independent maguTn10\tetR over-expression construct insertions yield increases in median life span inb oth males and females ranging from 2 to 18%. In contrast line P{tetR-magu}103 yeilded a 14% increase in median life span in males but no increase in females, while line P{tetR-magu}67-2 gave no increase in either sex.

In a Tn10\tetRAct5C.T:Hsim\VP16 background, two maguTn10\tetR lines P{tetR-magu}102 and P{tetR-magu}67-1 each give increases in median life span in both males and females, ranging fro 2.6 to 21%.

Two P{tetR-magu} insertions, P{tetR-magu}102 and P{tetR-magu}67-1, both yield increases in median life span in females of 28%, while no significant effect is found in males. In a background containing both Tn10\tetRM2.alt.Scer\UAS.T:Hsim\VP16 and Scer\GAL4D42 these insertions still show an increased life span in female flies but not male flies and an increase in median life span in both males and females, ranging from 9 to 37%.

maguTn10\tetR overexpression (in the presence of DOX) increases female fecundity in late adult life (from day 30 to day 40), but also results in reduced female fecundity in earlier life (days 10-20). This may be due to maternal-effect lethality, in that relatively normal quantities of morphologically normal appearing eggs are laid, but the majority of these eggs fail to hatch. There may also be some dose-dependent decreases in female fecundity at both time points.

maguTn10\tetR overexpression mutants (under the control of Scer\GAL4D42 and Tn10\tetRM2.alt.Scer\UAS.T:Hsim\VP16), where DOX administration is initiated at age 40 days, exhibit increased female fecundity at late ages at one or more DOX concentrations. The late-age administration of drug had no negative effect on female survival.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
maguTn10\tetO.cLa
maguTn10\tetR
magutetO.cLa
Name Synonyms
Tn10 tetracycline operator construct a of Li
Secondary FlyBase IDs
    References (2)