Allele Dmel\mre11^58S

General Information
Symbol	Dmel\mre11^58S	Species	D. melanogaster
Name		FlyBase ID	FBal0240645
Feature type	allele	Associated gene	Dmel\mre11

Allele class	hypomorphic allele - genetic evidence
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Gao et al., 2009)
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Dp(3;3)mre11^+.H230Y (Gao et al., 2009)
Nature of the lesion	Statement Reference
Carried in construct	P{mre11.H230Y} (Gao et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Gao et al., 2009) lethal \| maternal effect (Gao et al., 2009) male fertile (Gao et al., 2009) mitotic cell cycle defective (Gao et al., 2009)
Phenotype Manifest In

Detailed Description
	Statement Reference Despite the normal appearance of homozygous or hemizygous mre11[58S] females, they lay embryos that do not hatch (>10,000 embryos counted), even when mated to wild-type males, indicating maternal effect lethality. mre11[58S] animals display mild telomere-capping defects, with 0.2 associations per mre11[58S] nucleus, compared with the wild-type level of 0.04. Early mre11[58S] mutant embryos (those examined before cycle 7) appear to be mostly normal, with nuclei occasionally connected by chromosomal bridges (8%). As the embryos developed, more nuclei appear connected by bridges, and nuclei with abnormal DNA content become abundant. Sister nuclei separation failed in 69 out of 228 mitoses; likely, the result of unresolved chromosome bridges. Some of these polyploid nuclei apparently attempt to divide in the next mitosis, creating multiple-lobed nuclei. Mitotic bridges are observed in 38% of anaphases and telophases. Most late-stage mre11[58S] embryos display large nuclei-free areas, and their interior is filled with abnormally large and highly condensed nuclei. These embryos rarely develop signs of gastrulation. Unresolved telomere associations are the most likely mechanism leading to chromosome bridging. Telomere associations are unequivocally identified in 95.3% of mre11[58S] embryos. Covalent telomere fusions are abundant in mre11[58S] mutant embryos. Contrary to the severe capping defect in the embryos, mre11[58S] postembryonic animals develop mild telomere dysfunction, and are viable. (Gao et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference mre11^58S has mitotic cell cycle defective phenotype, enhanceable by nbs^Δ180 (Gao et al., 2009)
Enhancer of
Statement Reference mre11^58S is an enhancer of mitotic cell cycle defective phenotype of nbs^Δ180 (Gao et al., 2009)
Other
Statement Reference mre11^58S, nbs^Δ180 has lethal \| pupal stage phenotype (Gao et al., 2009, Gao et al., 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference nbs[Δ180] and mre11[58S] double mutants are pupal lethal, suffering a more severe telomere dysfunction than either single mutant (1.4 fusions per nucleus). (Gao et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mre11^58S (Gao et al., 2009, Gao et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Gao et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(26): 10728--10733 Mre11-Rad50-Nbs complex is required to cap telomeres during Drosophila embryogenesis. [FBrf0208278]
Supplementary material	Gao et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(26): Supporting Information. [FBrf0209441]

Allele Dmel\mre1158S

Allele Dmel\mre11^58S