Allele Dmel\Atf-2^{dsRNA.Scer\UAS.cOa}

General Information
Symbol	Dmel\Atf-2^{dsRNA.Scer\UAS.cOa}	Species	D. melanogaster
Name		FlyBase ID	FBal0240735
Feature type	allele	Associated gene	Dmel\Atf-2

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Okamura et al., 2007) in vitro construct - regulatory fusion (Okamura et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a 566bp inverted repeat of Atf-2 sequences (corresponding to nucleotides 59-624 of the coding sequence), in head-to-head orientation. (Okamura et al., 2007)
Carried in construct	P{UAS-Atf-2.RNAi.O} (Okamura et al., 2007, Shimizu et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective \| adult stage (Shimizu et al., 2008) small body \| adult stage, with Scer\GAL4^da.G32 (Okamura et al., 2007) stress response defective, with Scer\GAL4^c564 (Okamura et al., 2007) stress response defective, with Scer\GAL4^da.G32 (Okamura et al., 2007)
Phenotype Manifest In
	embryonic/larval fat body, with Scer\GAL4^c564 (Okamura et al., 2007) LNv neuron (Shimizu et al., 2008)
Detailed Description
	Statement Reference Locomotor activity is 59% higher than controls in flies expressing Atf-2[dsRNA.Scer\UAS.cOa] with Scer\GAL4[P2.4.Pdf], and these flies sleep less than controls under light/dark (408min a day compared to 563 for controls) or dark/dark conditions. These animals were found to have a lower arousal threshold than controls. (Shimizu et al., 2008) Expression of Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[Act] at 25[o]C does not cause lethality during the embryonic and larval stages. Flies expressing Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[da.G32] or Scer\GAL4[c564] have impaired survival under starvation conditions; they show a more rapid death than controls when deprived of all sources of energy after being aged 7 days. Flies expressing Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[Mhc.PW] show normal levels of survival under starvation conditions. The body weight of flies expressing Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[da.G32] is slightly (4%), but significantly, lower than that of controls. The morphology of the fat body cells is normal in flies Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[c564] and the fat body cells are normal in size. Flies expressing Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[c564] show reduced Nile-Red staining in the fat body compared to controls. Triglyceride levels of the whole body are 23% lower in 7 day old mutant flies than in control adults and are 28% lower in third instar mutant larvae than in control larvae. Hemolymph trehalose levels are similar in mutant and control flies. Fat bodies from third instar larvae expressing Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[c564] show a reduced rate of glyceroneogenesis (assayed by incorporation of pyruvate into lipids) compared to controls. Under starvation conditions, triglyceride levels drop at a similar rate in flies expressing Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[c564] and in controls. A high-calorie diet enhances the reduced body weight and reduced triglyceride levels phenotypes which are seen in animals expressing Atf-2[dsRNA.Scer\UAS.cOa] under the control of Scer\GAL4[c564]. (Okamura et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Atf-2^{dsRNA.Scer\UAS.cOa}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Shimizu et al., 2008, Mol. Cell. Biol. 28(20): 6278--6289 Drosophila ATF-2 regulates sleep and locomotor activity in pacemaker neurons. [FBrf0207384] Okamura et al., 2007, Mol. Biol. Cell 18(4): 1519--1529 ATF-2 regulates fat metabolism in Drosophila. [FBrf0201502]

Allele Dmel\Atf-2dsRNA.Scer\UAS.cOa

Allele Dmel\Atf-2^{dsRNA.Scer\UAS.cOa}