FB2025_01 , released February 20, 2025
Allele: Dmel\OraiUAS.cZa
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General Information
Symbol
Dmel\OraiUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Ziegenhorn
FlyBase ID
FBal0240749
Feature type
allele
Associated gene
Dmel\Orai
Associated Insertion(s)
Carried in Construct
P{UAS-Orai.Z}
Also Known As
UASdOrai, UASdOrai+, UAS-OraiWT
Key Links
Transgenic product class
wild_type
(Venkiteswaran and Hasan, 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Venkiteswaran and Hasan, 2009)
Mutagen
in vitro construct
(Venkiteswaran and Hasan, 2009)
Progenitor genotype
Carried in construct
P{UAS-Orai.Z}
Cytology
Description

UAS regulatory sequences drive expression of olf186-F coding sequences.

(Venkiteswaran and Hasan, 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Orai
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of olf186-FScer\UAS.cZa under the control of Scer\GAL4elav-C155 does not affect developmental rate.

      (Pathak et al., 2017)

      The expression of olf186-FScer\UAS.cZa under the control of Scer\GAL4ppk.PG results in third instar larval class IV abdominal dorsal multidendritic ddaC neurons exhibiting abnormal dendritic terminals, as compared to controls; expression under the control of Scer\GAL4221 results in no obvious dendrite morphology defects in class I abdominal dorsal multidendritic ddaD and ddaE neurons, as compared to controls.

      (Hattori et al., 2013)

      Overexpression of olf186-FScer\UAS.cZa in wild-type neurons (under the control of Scer\GAL4elav-C155) does not afffect SOCE and '[Ca[2+]'][[ER]], although it does elevate '[Ca[2+]'][[i]] to ~1υM in wild-type.

      (Venkiteswaran and Hasan, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of StimScer\UAS.cAa and olf186-FScer\UAS.cZa driven by Scer\GAL4Ilp2.PR completely suppresses the wing posture, flight and flight physiology defects of Itp-r83Aka1091/Itp-r83Aug3 flies.

      Simultaneous expression of StimScer\UAS.cAa and olf186-FScer\UAS.cZa driven by Scer\GAL4Ddc.PL in aminergic neurons results in pupal lethality.

      Pan-neuronal expression of StimScer\UAS.cAa and olf186-FScer\UAS.cZa driven by Scer\GAL4elav-C155 in Itp-r83Aka1091/Itp-r83Aug3 flies suppresses the abnormal wing-posture to varying extents but does not fully restore flight ability.

      Overexpression of olf186-FScer\UAS.cZa driven by Scer\GAL4Ilp2.PR in Itp-r83Aka1091/Itp-r83Aug3 mutant neurons fails to restore global intracellular Ca[2+] homeostasis.

      Simultaneous expression of StimScer\UAS.cAa and olf186-FScer\UAS.cZa driven by Scer\GAL4Ilp2.PR in Itp-r83Aka1091/Itp-r83Aug3 mutant neurons restores global intracellular Ca[2+] homeostasis.

      (Agrawal et al., 2010)

      Overexpression of olf186-FScer\UAS.cZa in wild-type neurons (under the control of Scer\GAL4elav-C155) elevates '[Ca[2+]'][[i]] levels to ~1υM in a Itp-r83Aka1091/Itp-r83Aug3 backgrounds.

      Expression of olf186-FScer\UAS.cZa in Ilp2 neurons (under the control of Scer\GAL4Ilp2.PR) partially suppresses the altered wing posture of Itp-r83Aka1091/Itp-r83Aug3 mutants. Although flight ability is not restored, flight patterns are initiated on air-puff delivery, while these are normally completely lacking in Itp-r83Aka1091/Itp-r83Aug3 mutants. Spontaneous hyperactivity of the DLMs in Itp-r83Aka1091/Itp-r83Aug3 mutants is suppressed to a significant extent by expression of olf186-FScer\UAS.cZa either ubiquitously (Scer\GAL4hs.PB at 25[o]C) or in the aminergic (Scer\GAL4Ilp2.PR) and glutamatergic subneuronal domains (Scer\GAL4OK371).

      Ubiquitous expression of olf186-FScer\UAS.cZa under the control of Scer\GAL4hs.PB in Itp-r83Aka1091/Itp-r83Aug3 mutants, up-regulated by a heat shock either in 24hr pupae or in 1-day-old adults results in a significant number of flies initiating flight in response to an air puff. Thus, levels of olf186-FScer\UAS.cZa can modulate flight circuit activity both during its development and in adult function. However, the flight patterns obtained are not sustained and appear arrhythmic, indicating that although olf186-FScer\UAS.cZa overexpression can suppress the flight defects and associated physiology of Itp-r83Aka1091/Itp-r83Aug3 mutants to a significant extent, it is insufficient to regain complete flight.

      Pan-neural overexpression of olf186-FScer\UAS.cZa in Itp-r83Aka1091/Itp-r83Aug3 neurons restores detectable SOCE to 70%, as in wild-type. Also, '[Ca[2+]'][[ER]] is restored to wild-type levels, indicating that reduced SOCE and elevated '[Ca[2+]'][[ER]] in Itp-r83Aka1091/Itp-r83Aug3 are linked homeostatic processes.

      Overexpression of olf186-FScer\UAS.cZa in wild-type neurons (under the control of Scer\GAL4elav-C155) does not afffect SOCE and '[Ca[2+]'][[ER]], although it does elevate '[Ca[2+]'][[i]] to ~1υM in wild-type and Itp-r83Aka1091/Itp-r83Aug3 backgrounds.

      (Venkiteswaran and Hasan, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/OraiUAS.cZa rescues OraiEY09167

      (Pathak et al., 2017)
      Partially rescues

      Scer\GAL4ple.PF/OraiUAS.cZa partially rescues OraiEY09167

      (Pathak et al., 2017)

      Scer\GAL4VGlut1-OK371/OraiUAS.cZa partially rescues OraiEY09167

      (Pathak et al., 2017)
      Fails to rescue

      Scer\GAL4dimm-929/OraiUAS.cZa fails to rescue OraiEY09167

      (Pathak et al., 2017)

      Scer\GAL4Mef2.PR/OraiUAS.cZa fails to rescue OraiEY09167

      (Pathak et al., 2017)
      Comments

      The developmental delay, small body size and partial lethality of olf186-FEY09167 homozygous mutants is rescued by expression of olf186-FScer\UAS.cZa under the control of the Scer\GAL4elav-C155 driver. The lethality is also partially rescued when the expression is driven by either Scer\GAL4ple.PF or Scer\GAL4VGlut-OK371, no rescue is observed with either Scer\GAL4dimm-929 or Scer\GAL4Mef2.PR drivers.

      (Pathak et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      OraiUAS.cZa
      olf186-FScer\UAS.cZa
      olf186-FUAS.cZa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Ziegenhorn
      Secondary FlyBase IDs
        References (8)