A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Allele Dmel\olf186-FScer\UAS.cZa

General Information
SymbolDmel\olf186-FScer\UAS.cZaSpeciesD. melanogaster
NameSaccharomyces cerevisiae UAS construct a of ZiegenhornFlyBase IDFBal0240749
Feature typealleleAssociated geneDmel\olf186-F
Allele class
Mutagenin vitro construct - regulatory fusion
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Description
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FB2013_03
FB2013_02
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hide Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Venkiteswaran and Hasan, 2009)
Mutations Mapped to the Genome
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion
Statement
Reference
Scer\UAS regulatory sequences drive expression of olf186-F coding sequences.
(Venkiteswaran and Hasan, 2009)
Carried in construct
P{UAS-Orai.Z}
(Venkiteswaran and Hasan, 2009, Agrawal et al., 2010)
Cytology
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Statement
Reference
Overexpression of olf186-F[Scer\UAS.cZa] in wild-type neurons (under the control of Scer\GAL4[elav-C155]) does not afffect SOCE and '[Ca[2+]'][[ER]], although it does elevate '[Ca[2+]'][[i]] to ~1υM in wild-type.
(Venkiteswaran and Hasan, 2009)
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Linkouts
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hide Phenotypic Class
hideSuppressor of
Statement
Reference
Scer\GAL4elav-C155, StimScer\UAS.cAa, olf186-FScer\UAS.cZa is a suppressor | partially of flight defective phenotype of Itp-r83Aug3/Itp-r83Aka1091
(Agrawal et al., 2010)
Scer\GAL4elav-C155, StimScer\UAS.cAa, olf186-FScer\UAS.cZa is a suppressor | partially of neurophysiology defective phenotype of Itp-r83Aug3/Itp-r83Aka1091
(Agrawal et al., 2010)
StimScer\UAS.cAa, Scer\GAL4Ilp2.PR, olf186-FScer\UAS.cZa is a suppressor of flight defective phenotype of Itp-r83Aug3/Itp-r83Aka1091
(Agrawal et al., 2010)
StimScer\UAS.cAa, Scer\GAL4Ilp2.PR, olf186-FScer\UAS.cZa is a suppressor of neurophysiology defective phenotype of Itp-r83Aug3/Itp-r83Aka1091
(Agrawal et al., 2010)
StimScer\UAS.cAa, Scer\GAL4Ilp2.PR, olf186-FScer\UAS.cZa is a suppressor of visible phenotype of Itp-r83Aug3/Itp-r83Aka1091
(Agrawal et al., 2010)
hideOther
Statement
Reference
Scer\GAL4Ddc.PL, StimScer\UAS.cAa, olf186-FScer\UAS.cZa has lethal | P-stage phenotype
(Agrawal et al., 2010)
hide Phenotype Manifest In
hideSuppressor of
Statement
Reference
Scer\GAL4elav-C155, StimScer\UAS.cAa, olf186-FScer\UAS.cZa is a suppressor | partially of wing phenotype of Itp-r83Aug3/Itp-r83Aka1091
(Agrawal et al., 2010)
StimScer\UAS.cAa, Scer\GAL4Ilp2.PR, olf186-FScer\UAS.cZa is a suppressor of wing phenotype of Itp-r83Aug3/Itp-r83Aka1091
(Agrawal et al., 2010)
hide Additional Comments
hide Genetic Interactions
Statement
Reference
Co-expression of Stim[Scer\UAS.cAa] and olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[Ilp2.PR] completely suppresses the wing posture, flight and flight physiology defects of Itp-r83A[ka1091]/Itp-r83A[ug3] flies. Simultaneous expression of Stim[Scer\UAS.cAa] and olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[Ddc.PL] in aminergic neurons results in pupal lethality. Pan-neuronal expression of Stim[Scer\UAS.cAa] and olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[elav-C155] in Itp-r83A[ka1091]/Itp-r83A[ug3] flies suppresses the abnormal wing-posture to varying extents but does not fully restore flight ability. Overexpression of olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[Ilp2.PR] in Itp-r83A[ka1091]/Itp-r83A[ug3] mutant neurons fails to restore global intracellular Ca[2+] homeostasis. Simultaneous expression of Stim[Scer\UAS.cAa] and olf186-F[Scer\UAS.cZa] driven by Scer\GAL4[Ilp2.PR] in Itp-r83A[ka1091]/Itp-r83A[ug3] mutant neurons restores global intracellular Ca[2+] homeostasis.
(Agrawal et al., 2010)
Overexpression of olf186-F[Scer\UAS.cZa] in wild-type neurons (under the control of Scer\GAL4[elav-C155]) elevates '[Ca[2+]'][[i]] levels to ~1υM in a Itp-r83A[ka1091]/Itp-r83A[ug3] backgrounds. Expression of olf186-F[Scer\UAS.cZa] in Ilp2 neurons (under the control of Scer\GAL4[Ilp2.PR]) partially suppresses the altered wing posture of Itp-r83A[ka1091]/Itp-r83A[ug3] mutants. Although flight ability is not restored, flight patterns are initiated on air-puff delivery, while these are normally completely lacking in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants. Spontaneous hyperactivity of the DLMs in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants is suppressed to a significant extent by expression of olf186-F[Scer\UAS.cZa] either ubiquitously (Scer\GAL4[hs.PB] at 25[o]C) or in the aminergic (Scer\GAL4[Ilp2.PR]) and glutamatergic subneuronal domains (Scer\GAL4[OK371]). Ubiquitous expression of olf186-F[Scer\UAS.cZa] under the control of Scer\GAL4[hs.PB] in Itp-r83A[ka1091]/Itp-r83A[ug3] mutants, up-regulated by a heat shock either in 24hr pupae or in 1-day-old adults results in a significant number of flies initiating flight in response to an air puff. Thus, levels of olf186-F[Scer\UAS.cZa] can modulate flight circuit activity both during its development and in adult function. However, the flight patterns obtained are not sustained and appear arrhythmic, indicating that although olf186-F[Scer\UAS.cZa] overexpression can suppress the flight defects and associated physiology of Itp-r83A[ka1091]/Itp-r83A[ug3] mutants to a significant extent, it is insufficient to regain complete flight. Pan-neural overexpression of olf186-F[Scer\UAS.cZa] in Itp-r83A[ka1091]/Itp-r83A[ug3] neurons restores detectable SOCE to 70%, as in wild-type. Also, '[Ca[2+]'][[ER]] is restored to wild-type levels, indicating that reduced SOCE and elevated '[Ca[2+]'][[ER]] in Itp-r83A[ka1091]/Itp-r83A[ug3] are linked homeostatic processes. Overexpression of olf186-F[Scer\UAS.cZa] in wild-type neurons (under the control of Scer\GAL4[elav-C155]) does not afffect SOCE and '[Ca[2+]'][[ER]], although it does elevate '[Ca[2+]'][[i]] to ~1υM in wild-type and Itp-r83A[ka1091]/Itp-r83A[ug3] backgrounds.
(Venkiteswaran and Hasan, 2009)
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Reference
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Discoverer
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Other Crossreferences
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
olf186-FScer\UAS.cZa
 
Name Synonym
Saccharomyces cerevisiae UAS construct a of Ziegenhorn
 
Secondary FlyBase IDs
hide References ( 2 )
Research paper
Agrawal et al., 2010, J. Neurosci. 30(4): 1301--1313
Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight. [FBrf0209847]
Venkiteswaran and Hasan, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(25): 10326--10331
Intracellular Ca2+ signaling and store-operated Ca2+ entry are required in Drosophila neurons for flight. [FBrf0208231]