Allele Dmel\Pld^Scer\UAS.cRa

General Information
Symbol	Dmel\Pld^Scer\UAS.cRa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Raghu	FlyBase ID	FBal0241089
Feature type	allele	Associated gene	Dmel\Pld

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Raghu et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the Pld open reading frame. (Raghu et al., 2009)
Carried in construct	P{UAS-Pld.R} (Raghu et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	eye photoreceptor cell, with Scer\GAL4^GMR.PF (Raghu et al., 2009) rhabdomere, with Scer\GAL4^GMR.PF (Raghu et al., 2009)
Detailed Description
	Statement Reference Flies expressing Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF] show vesiculation and degeneration of the rhabdomeres. In addition, the cell body of the photoreceptors shows accumulation of expanded tubulovesicular membraneous structures, which appear as concentric stacks of membranes in the most severe cases. Retinal extracts from flies expressing Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF] show a significant elevation of a single species of phosphatidic acid (predicted fatty acyl composition 16:0/18:2) compared to controls. The levels of phosphatidylcholine and phosphatidylinositol in the retinal extracts are normal. (Raghu et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Pld^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has rhabdomere phenotype, enhanceable by garz^EP2028, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Raghu et al., 2009)
NOT Enhanced by
Statement Reference Pld^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, non-enhanceable by siz^P2.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Raghu et al., 2009)
Suppressed by
Statement Reference Pld^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has rhabdomere phenotype, suppressible \| partially by CG11426^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Raghu et al., 2009) Pld^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has rhabdomere phenotype, suppressible by sktl^Δ1-1/sktl^Δ20 (Raghu et al., 2009)
NOT suppressed by
Statement Reference Pld^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, non-suppressible by Arf51F⁴ (Raghu et al., 2009) Pld^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has rhabdomere phenotype, non-suppressible by shi¹ (Raghu et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of CG11426[Scer\UAS.T:Avic\GFP] partially rescues the rhabdomere degeneration caused by expression of Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF]. Co-expression of garz[EP2028] strongly enhances the retina phenotype caused by expression of Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF]; only one or two poorly developed rhabdomeres are seen. Co-expression of Gap69C[Scer\UAS.cRa] partially the rhabdomere defects caused by expression of Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF]. Co-expression of siz[P2.Scer\UAS] does not enhance the photoreceptor cell phenotypes caused by expression of Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF]. Arf51F[4] does not suppress the photoreceptor cell phenotypes caused by expression of Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF]. Animals co-expressing both Pld[Scer\UAS.cRa] and α-Adaptin[dsRNA.Scer\UAS.cUa] under the control of Scer\GAL4[GMR.PF] have virtually no detectable rhabdomeres, and the cell bodies of the photoreceptors continue to show accumulation of membrane transport intermediates. A shi[1] background at 23[o]C does not suppress the rhabdomere defects caused by expression of Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF]. There is a dramatic increase in rhabdomere size in flies expressing Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF] in a sktl[Δ1-1]/sktl[Δ20] background compared to flies expressing Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF] in a wild-type background. (Raghu et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Pld^Scer\UAS.cRa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Raghu
Secondary FlyBase IDs

References ( 1 )
Research paper	Raghu et al., 2009, J. Cell Biol. 185(1): 129--145 Rhabdomere biogenesis in Drosophila photoreceptors is acutely sensitive to phosphatidic acid levels. [FBrf0207819]

Allele Dmel\PldScer\UAS.cRa

Allele Dmel\Pld^Scer\UAS.cRa