FB2025_01 , released February 20, 2025
Allele: Dmel\SmnUAS.Tag:FLAG
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General Information
Symbol
Dmel\SmnUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0241133
Feature type
allele
Associated gene
Dmel\Smn
Associated Insertion(s)
Carried in Construct
P{UAS-Smn.FLAG}
Also Known As
UAS-Flag-Smn, UAS-SMN
Key Links
Transgenic product class
wild_type
(Chang et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Chang et al., 2008)
Mutagen
in vitro construct
(Chang et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Smn.FLAG}
Cytology
Description

UAS regulatory sequences drive expression of Smn which is tagged at the N-terminal end with Tag:FLAG.

(Chang et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Smn
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mef2.PR has no effect on flight ability of adult flies, mobility of the third instar larvae or shape of pupae and Scer\GAL4elav-C155-driven expression does not impair adult viability.

      (Lanfranco et al., 2017)

      Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU leads to mild defects in eye morphology.

      (Mirra et al., 2017)

      Expression of SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4Mef2.PR does not significantly affect flight behavior.

      (Borg et al., 2015)

      Overexpression of SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155, Scer\GAL4how-24B, Scer\GAL4VGlut-OK371 or Scer\GAL4ChAT.7.4 does not significantly disrupt locomotor behavior (speed, distance covered, overall activity, pause number and length in Buridan's assay) in adult flies compared to wild type.

      Baseline synaptic transmission measured at the neuromuscular junction (muscle 6/7 in abdominal segment 2) is mildly but significantly altered in third instar larvae with SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155 : though no changes in excitatory junction potential (EJP) amplitude or the quantal content of release were detected, there is a significant increase in mini evoked junction potential (mEJP) amplitude. When SmnScer\UAS.T:Zzzz\FLAG is driven in muscles by Scer\GAL4how-24B there are significant decreases in EJP and mEJP amplitude.

      (Timmerman and Sanyal, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Simultaneous expression of Gem3ΔN.Scer\UAS and SmnScer\UAS.T:Zzzz\FLAG leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium with the Scer\GAL4Mef2.PR driver and reduced adult viability with Scer\GAL4elav-C155.

      (Lanfranco et al., 2017)

      Co-expression of SmnScer\UAS.T:Zzzz\FLAG and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in partial lethality, with the few escapers exhibiting severe flight defects.

      (Borg et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of SmnScer\UAS.T:Zzzz\FLAG does not modify the mild eye defects seen in flies expressing Hsap\FUSMM.Scer\UAS.T:Zzzz\FLAG or Hsap\FUSScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU.

      (Mirra et al., 2017)
      Complementation and Rescue Data
      Rescues

      SmnY203C.Tag:FLAG, SmnTag:FLAG, Scer\GAL4Act.PU, SmnUAS.Tag:FLAG rescues SmnX7

      (Praveen et al., 2014)

      Scer\GAL4da.PU/SmnUAS.Tag:FLAG rescues SmnX7

      (Imlach et al., 2012)

      SmnUAS.Tag:FLAG/Scer\GAL4nSyb.PS rescues SmnX7

      (Imlach et al., 2012)

      SmnUAS.Tag:FLAG/Scer\GAL4ChAT.7.4 rescues SmnX7

      (Imlach et al., 2012)

      Scer\GAL4Act.PU/SmnUAS.Tag:FLAG rescues Smnf05960

      (Chang et al., 2008)

      SmnUAS.Tag:FLAG/Scer\GAL4Tub.PU rescues Smnf05960

      (Chang et al., 2008)
      Partially rescues

      SmnUAS.Tag:FLAG/Scer\GAL4clh201 partially rescues SmnX7

      (Imlach et al., 2012)

      Scer\GAL4NP2225/SmnUAS.Tag:FLAG partially rescues SmnX7

      (Imlach et al., 2012)

      Scer\GAL4elav.Switch.PO/SmnUAS.Tag:FLAG partially rescues SmnX7

      (Imlach et al., 2012)

      Scer\GAL4Act.PU/SmnUAS.Tag:FLAG partially rescues Smn73Ao

      (Chang et al., 2008)

      SmnUAS.Tag:FLAG/Scer\GAL4Tub.PU partially rescues Smn73Ao

      (Chang et al., 2008)

      SmnUAS.Tag:FLAG/Scer\GAL4elav.PLu partially rescues Smn73Ao/Smnf01109

      (Chang et al., 2008)

      Scer\GAL4how-24B/SmnUAS.Tag:FLAG partially rescues Smn73Ao/Smnf01109

      (Chang et al., 2008)
      Fails to rescue

      Scer\GAL4G14/SmnUAS.Tag:FLAG fails to rescue SmnX7

      (Imlach et al., 2012)

      SmnUAS.Tag:FLAG/Scer\GAL4VGlut1-OK371 fails to rescue SmnX7

      (Imlach et al., 2012)

      SmnUAS.Tag:FLAG/Scer\GAL4RapGAP1-OK6 fails to rescue SmnX7

      (Imlach et al., 2012)

      Scer\GAL4Gad1.3.098/SmnUAS.Tag:FLAG fails to rescue SmnX7

      (Imlach et al., 2012)

      SmnUAS.Tag:FLAG/Scer\GAL41003.3 fails to rescue SmnX7

      (Imlach et al., 2012)

      SmnUAS.Tag:FLAG/Scer\GAL4ppk.1.9 fails to rescue SmnX7

      (Imlach et al., 2012)
      Comments

      Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4Act.PU or Scer\GAL4tub.PU partially rescues the semi-lethality seen when one copy each of SmnT:Zzzz\FLAG and SmnY203C.T:Zzzz\FLAG are expressed in a homozygous SmnX7 mutant background.

      (Praveen et al., 2014)

      Decreased muscle surface area, locomotor defects, increased inter-spike intervals and increased evoked excitatory postsynaptic potential (eEPSP) amplitude in SmnX7/SmnX7 third instar larvae are rescued by ubiquitous (Scer\GAL4da.PU), pan-neuronal (Scer\GAL4nSyb.PS) or cholinergic (Scer\GAL4ChAT.7.4) expression of SmnScer\UAS.T:Zzzz\FLAG; these phenotypes are not rescued when expression is driven by larval muscle-specific Scer\GAL4G14, glutamatergic Scer\GAL4VGlut-OK371, motor-neuron specific Scer\GAL4RapGAP1-OK6, GABAergic (Scer\GAL4Gad1.3.098), Scer\GAL41003.3 or Scer\GAL4ppk.1.9. Expression of SmnScer\UAS.T:Zzzz\FLAG with Scer\GAL4clh201 or Scer\GAL4NP2225 rescues the increased inter-spike intervals and increased eEPSP amplitude, partially rescues the decreased muscle surface area, but fails to rescue the locomotion defect in SmnX7/SmnX7 third instar larvae.

      SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav.Switch.PO in the presence of RU486 rescues SmnX7/SmnX7 muscle size, locomotion (partially), inter-spike intervals and eEPSP defects if SmnScer\UAS.T:Zzzz\FLAG expression is induced immediately after hatching (if induced 48 or 96 hours after hatching rescue is partial apart from full rescue of eEPSP).

      (Imlach et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      SmnScer\UAS.T:Zzzz\FLAG
      SmnUAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (10)