Allele Hsap\ORC6^T:Avic\GFP

General Information
Symbol	Hsap\ORC6^T:Avic\GFP	Species	H. sapiens
Name		FlyBase ID	FBal0241173
Feature type	allele	Associated gene	Hsap\ORC6

Allele class
Mutagen	in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Balasov et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Full-length Orc6 coding sequence, under the control of the native Orc6 promoter is fused with T:Avic\GFP coding sequences. (Balasov et al., 2009)
Carried in construct	P{HsOrc6-GFP} (Balasov et al., 2009)
Tagged with	T:Avic\GFP (Balasov et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Hsap\ORC6^T:Avic\GFP is a suppressor of mitotic cell cycle defective phenotype of Orc6³⁵ (Balasov et al., 2009)
NOT Suppressor of
Statement Reference Hsap\ORC6^T:Avic\GFP is a non-suppressor of lethal \| third instar larval stage phenotype of Orc6³⁵ (Balasov et al., 2009)
Phenotype Manifest In
Enhancer of
Statement Reference Hsap\ORC6^T:Avic\GFP is an enhancer of embryonic/larval brain phenotype of Orc6³⁵ (Balasov et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\ORC6[T:Avic\GFP] does not rescue viability in Orc6[35] mutants (that die at the third instar larval stage). Expression of Hsap\ORC6[T:Avic\GFP] results in the release of Orc6[35] mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings. Chromosomes from neuroblasts isolated from Orc6[35] mutants carrying Hsap\ORC6[T:Avic\GFP] are able to incorporate BrdU, and therefore are able to replicate DNA. b Brains observed in these larvae are significantly reduced in size compared with heterozygous or wild-type larval brains, suggesting severe defects in cell proliferation and larval brain development. Orc6[35] mutant neuroblasts carrying Hsap\ORC6[T:Avic\GFP] accumulate at mitosis. The number of mitotic neuroblasts derived from these transgenic flies increases approximately 2-fold compared with Orc6[35] mutant cells. (Balasov et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\ORC6^T:Avic\GFP
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Balasov et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(26): 10672--10677 Functional analysis of an Orc6 mutant in Drosophila. [FBrf0208195]

Allele Hsap\ORC6T:Avic\GFP

Allele Hsap\ORC6^T:Avic\GFP