Allele Hsap\CHMP2B^{Intron5.Scer\UAS}

General Information
Symbol	Hsap\CHMP2B^{Intron5.Scer\UAS}	Species	H. sapiens
Name		FlyBase ID	FBal0241178
Feature type	allele	Associated gene	Hsap\CHMP2B

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Ahmad et al., 2009) in vitro construct - regulatory fusion (Ahmad et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\CHMP2B coding sequences in which the final 35 amino acid at the C-terminal are removed. (Ahmad et al., 2009)
Carried in construct	P{UAS-CHMP2B.ΔC} (Ahmad et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death, with Scer\GAL4^GMR.PF (Ahmad et al., 2009) lethal, with Scer\GAL4^elav-C155 (Ahmad et al., 2009)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Ahmad et al., 2009) photoreceptor, with Scer\GAL4^GMR.PF (Ahmad et al., 2009)
Detailed Description
	Statement Reference Pan-neural expression of Hsap\CHMP2B[Intron5.Scer\UAS] results in lethality. Expression of Hsap\CHMP2B[Intron5.Scer\UAS] in the eye, under the control of Scer\GAL4[GMR.PF] results in a rough eye phenotype and the appearance of black spots. Internal eye structure is also disrupted. (Ahmad et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by +/Df(2L)ED1243, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by cact[+]/cact¹, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by cact[+]/cact^KG07677, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by cact⁴/cact[+], Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by CG9779[+]/vps24^EY04708, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Df(3L)ED210/+, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Df(3R)ED5664/+, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Mmus\Chmp4b^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by shrb[+]/shrb⁴, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Spn88Ea^c01214, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Spn88Ea^c01214/Spn5[+], Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Spn88Ea^GS9853, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by TSG101[+]/TSG101^f00976, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by vps2^GS11024/vps2[+], Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Vps28[+]/Vps28^k16503, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
NOT Enhanced by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, non-enhanceable by Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
Suppressed by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Spn88Ea^c01214/Spn5[+] has increased cell death phenotype, suppressible by Spn88Ea^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
NOT suppressed by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, non-suppressible by Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
Enhancer of
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Mmus\Chmp4b^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by +/Df(2L)ED1243, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by cact[+]/cact¹, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by cact[+]/cact^KG07677, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by cact⁴/cact[+], Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by CG9779[+]/vps24^EY04708, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(3L)ED210/+, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(3R)ED5664/+, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Mmus\Chmp4b^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by shrb[+]/shrb⁴, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Spn88Ea^c01214, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Spn88Ea^c01214/Spn5[+], Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Spn88Ea^GS9853, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by TSG101[+]/TSG101^f00976, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by vps2^GS11024/vps2[+], Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Vps28[+]/Vps28^k16503, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
NOT Enhanced by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
Suppressed by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Spn88Ea^c01214/Spn5[+] has eye phenotype, suppressible by Spn88Ea^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible by Bc¹/Bc[+], Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible by Spn88Ea^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
NOT suppressed by
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009) Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has photoreceptor phenotype, non-suppressible by Spn88Ea^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
Enhancer of
Statement Reference Hsap\CHMP2B^{Intron5.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Mmus\Chmp4b^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ahmad et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Mmus\Chmp4b with Hsap\CHMP2B[Intron5.Scer\UAS] greatly enhances the severity of the rough eye phenotype compared to both single mutants (both under the control of Scer\GAL4[GMR.PF]). Expression of Mmus\Cd8a[Scer\UAS.T:Avic\GFP] with Hsap\CHMP2B[Intron5.Scer\UAS] does not affect the severity of the rough eye phenotype (both under the control of Scer\GAL4[GMR.PF]). A single copy of shrb[4], vps2[GS11024], vps24[EY04708], Vps28[k16503], TSG101[f00976] or Df(3L)ED210 significantly enhances the Hsap\CHMP2B[Intron5.Scer\UAS] Scer\GAL4[GMR.PF] phenotype in 1-day-old flies. The Hsap\CHMP2B[Intron5.Scer\UAS] Scer\GAL4[GMR.PF] eye phenotype is significantly enhanced by a Df(2L)ED1243/+ or Df(3R)ED5664/+ background. Spn5[c01214] significantly enhances the Hsap\CHMP2B[Intron5.Scer\UAS] Scer\GAL4[GMR.PF] eye phenotype. Spn5[GS9853] significantly enhances the Hsap\CHMP2B[Intron5.Scer\UAS] Scer\GAL4[GMR.PF] eye phenotype. The Scer\GAL4[GMR.PF] Hsap\CHMP2B[Intron5.Scer\UAS]-mediated eye black spots are suppressed in a Bc[1]/+ background. The presence of one copy of spz[2] or spz[KG05402] markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2B[Intron5.Scer\UAS] under the control of Scer\GAL4[GMR.PF]. The presence of a copy of different cact mutant alleles, cact[1], cact[4], or cact[KG07677] significantly enhanced the Hsap\CHMP2B[Intron5.Scer\UAS] Scer\GAL4[GMR.PF] phenotype. Expression of Spn5[Scer\UAS.cAa] in the eye under the control of Scer\GAL4[GMR.PF] suppresses the external eye phenotype caused by Hsap\CHMP2B[Intron5.Scer\UAS] expression. However, Spn5[Scer\UAS.cAa] overexpression does not rescue the photoreceptor degeneration phenotype. Expression of Spn5[Δsig.Scer\UAS] in the eye under the control of Scer\GAL4[GMR.PF] fails to suppress the melanization phenotype caused by Hsap\CHMP2B[Intron5.Scer\UAS] expression. Expression of Spn5[Scer\UAS.cAa] in the eye under the control of Scer\GAL4[GMR.PF] completely suppresses the enhancement effect of Spn5[c01214]/+ on the Hsap\CHMP2B[Intron5.Scer\UAS] locus. The presence of one copy of spz[2] markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2B[Intron5.Scer\UAS] under the control of Scer\GAL4[GMR.PF]. The presence of one copy of spz[KG05402] markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2B[Intron5.Scer\UAS] under the control of Scer\GAL4[GMR.PF]. (Ahmad et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\CHMP2B^{Intron5.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Ahmad et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(29): 12168--12173 Genetic screen identifies serpin5 as a regulator of the toll pathway and CHMP2B toxicity associated with frontotemporal dementia. [FBrf0208495]

Allele Hsap\CHMP2BIntron5.Scer\UAS

Allele Hsap\CHMP2B^{Intron5.Scer\UAS}