Allele Dmel\CTCF^{Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\CTCF^{Scer\UAS.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0241204
Feature type	allele	Associated gene	Dmel\CTCF

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Mohan et al., 2007) in vitro construct - regulatory fusion (Mohan et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of CTCF coding sequences fused with T:Avic\GFP[EGFP] sequences at the C-terminal end. (Mohan et al., 2007)
Carried in construct	P{UAS-CTCF-EGFP} (Mohan et al., 2007)
Tagged with	T:Avic\GFP^EGFP (Mohan et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^Act5C.PI (Mohan et al., 2007) lethal \| embryonic stage, with Scer\GAL4^αTub84B.PL (Mohan et al., 2007)
Phenotype Manifest In

Detailed Description
	Statement Reference Ubiquitous expression of CTCF[Scer\UAS.T:Avic\GFP-EGFP] in a wild-type background with the drivers Scer\GAL4[αTub84B.PL] or Scer\GAL4[Act5C.PI] results in early embryonic lethality. (Mohan et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments	A single heat shock (37[o]C) for 20 mins at 24, 48, or 72 hours into development is sufficient to phenotypically rescue the majority of flies with a CTCF[P30.6]/Df(3L)0463 or CTCF[GE24185]/Df(3L)0463 background when CTCF[Scer\UAS.T:Avic\GFP-EGFP] and Scer\GAL4[hs.PB] are present. Both sexes are fertile. A few flies exhibit a very mild held-out wing phenotype and some have a disorganized bristle pattern on the dorsal abdomen. Heat shock at the pupal stage could not rescue all the mutant phenotypes. (Mohan et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	CTCF^{Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Mohan et al., 2007, EMBO J. 26(19): 4203--4214 The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning. [FBrf0200449]

Allele Dmel\CTCFScer\UAS.T:Avic\GFP-EGFP

Allele Dmel\CTCF^{Scer\UAS.T:Avic\GFP-EGFP}