FlyBase Allele Report: Dmel\prel[1]

General Information

Symbol

Dmel\prel¹

Species

D. melanogaster

Name

FlyBase ID

FBal0241221

Feature type

allele

Associated gene

Dmel\prel

Associated Insertion(s)

Carried in Construct

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Tsubouchi et al., 2009)

Mutagen

P-element activity

(Tsubouchi et al., 2009)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{GSV6}prel^GS14515

(Tsubouchi et al., 2009)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of the progenitor insertion, resulting in a 1452bp deletion that removes almost the entire prel open reading frame.

(Tsubouchi et al., 2009)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Tsubouchi et al., 2009)

abnormal neuroanatomy | third instar larval stage

(Tsuyama et al., 2017)

lethal - all die before end of larval stage | recessive

(Tsubouchi et al., 2009)

some die during larval stage | recessive

(Tsubouchi et al., 2009)

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaA | third instar larval stage

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaC | third instar larval stage

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaE | third instar larval stage

(Tsuyama et al., 2017)

dendrite & dorsal multidendritic neuron ddaC | somatic clone

(Tsubouchi et al., 2009)

dendrite | third instar larval stage

(Tsuyama et al., 2017)

mitochondrion & dorsal multidendritic neuron ddaC | somatic clone

(Tsubouchi et al., 2009)

mitochondrion | third instar larval stage

(Tsuyama et al., 2017)

Detailed Description

Statement

Reference

prel¹ mutant third instar larvae display fragmentation of mitochondria in the cell body and shortening of dendritic arbors in the class IV ddaC neurons, the reduction of dendritic arbor is also observed in class I ddaE and class III ddaA neurons although the phenotype in these two neuronal classes is much weaker.

(Tsuyama et al., 2017)

Homozygotes die as early larvae.

The density of mitochondria (assayed by the expression of markers with a mitochondrial targeting signal) is significantly reduced in both axons and dendrites of homozygous ddaC neuron clones compared to controls. The mitochondria appear to be fragmented in the mutant neurons.

Homozygous ddaC neuron clones have shorter, smaller and much less-branched dendritic arbors compared to control neurons. The terminal branches tend to be shorter than those of wild-type neurons, making the mutant distal dendrites appear fuzzy. Some detached branches are seen in the mutant arbor, with the breakages occurring more frequently in the proximal area of the arbor than in the distal area. No axonal breakage (at least not close to the cell body) is seen in the mutant neurons.

(Tsubouchi et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

prel¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by PPP1R15^UAS.cMa/Scer\GAL4^ppk.PU

(Tsuyama et al., 2017)

prel¹ has abnormal neuroanatomy | somatic clone phenotype, suppressible by Buffy^UAS.cQa/Scer\GAL4^ppk.1.9

(Tsubouchi et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

prel¹ has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by PPP1R15^UAS.cMa/Scer\GAL4^ppk.PU

(Tsuyama et al., 2017)

prel¹ has dendrite | third instar larval stage phenotype, suppressible | partially by PPP1R15^UAS.cMa/Scer\GAL4^ppk.PU

(Tsuyama et al., 2017)

prel¹ has dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype, suppressible by Buffy^UAS.cQa/Scer\GAL4^ppk.1.9

(Tsubouchi et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of class IV ddaC dendritic arbor characteristic for prel¹ third instar larvae can be significantly mitigated by expression of PPP1R15^Scer\UAS.cMa under the control of Scer\GAL4^ppk.PU.

(Tsuyama et al., 2017)

Expression of Buffy^Scer\UAS.cQa under the control of Scer\GAL4^ppk.1.9 suppresses the abnormal morphology of the dendritic arbors of prel¹ homozygous ddaC neuron clones.

(Tsubouchi et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

prel¹ is rescued by Scer\GAL4^da.G32/prel^UAS.Tag:HA

(Tsubouchi et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

prel¹

(Tsuyama et al., 2017, Tsubouchi et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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