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General Information
Symbol
Dmel\prel1
Species
D. melanogaster
Name
FlyBase ID
FBal0241221
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 1452bp deletion that removes almost the entire prel open reading frame.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

prel1 mutant third instar larvae display fragmentation of mitochondria in the cell body and shortening of dendritic arbors in the class IV ddaC neurons, the reduction of dendritic arbor is also observed in class I ddaE and class III ddaA neurons although the phenotype in these two neuronal classes is much weaker.

Homozygotes die as early larvae.

The density of mitochondria (assayed by the expression of markers with a mitochondrial targeting signal) is significantly reduced in both axons and dendrites of homozygous ddaC neuron clones compared to controls. The mitochondria appear to be fragmented in the mutant neurons.

Homozygous ddaC neuron clones have shorter, smaller and much less-branched dendritic arbors compared to control neurons. The terminal branches tend to be shorter than those of wild-type neurons, making the mutant distal dendrites appear fuzzy. Some detached branches are seen in the mutant arbor, with the breakages occurring more frequently in the proximal area of the arbor than in the distal area. No axonal breakage (at least not close to the cell body) is seen in the mutant neurons.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The shortening of class IV ddaC dendritic arbor characteristic for prel1 third instar larvae can be significantly mitigated by expression of PPP1R15Scer\UAS.cMa under the control of Scer\GAL4ppk.PU.

Expression of BuffyScer\UAS.cQa under the control of Scer\GAL4ppk.1.9 suppresses the abnormal morphology of the dendritic arbors of prel1 homozygous ddaC neuron clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (2)