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General Information
Symbol
Dmel\tutl23
Species
D. melanogaster
Name
FlyBase ID
FBal0241228
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
Deletion of part of tutl resulting from FLP-catalyzed recombination using the FRT-containing transposons PBac{WH}tutlf03096 and PBac{WH}tutlf02770.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference
Scer\FRT-mediate recombination between the two progenitor insertions has resulted in a deletion that removes amino acid residues Glu133 to Ala754 (the five Ig-like domains and the first fibronectin-type-III repeat) of tutl.
Recombination between the two progenitor insertions, resulting in a deletion of the intervening DNA between them and the reconstitution of the inserted element at the deletion site.
Insertion components
PBac{WHr}tutl23
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
dendrite & dorsal multidendritic neuron ddaC (with tutl01085)
dendrite & dorsal multidendritic neuron ddaC | somatic clone
dendrite & dorsal multidendritic neuron ddaE
dendrite & dorsal multidendritic neuron ddaE (with Df(2L)ed-dp)
dendrite & dorsal multidendritic neuron ddaE (with tutl01085)
dendrite & dorsal multidendritic neuron ddaE | somatic clone
Detailed Description
Statement
Reference
Unlike in wild-type, many R7 terminals stick with neighbouring R7 terminals in homozygous tutl23 mutants.
Homozygous, tutl23/tutl01085 and tutl23/Df(2L)tutl larvae show a severe reduction compared to wild-type controls in changing moving direction in response to gentle touch at anterior segments. The number of exploratory head swings in response to the touch, and the time taken to select a new moving direction are increased. The withdrawal response to the gentle touch is normal. Locomotory behavior in a stimulus-free condition is normal in homozygous and tutl23/tutl01085 larvae (number of contractions, average speed, number of turns and average turning angles have been analysed). Homozygous and tutl23/tutl01085 larvae show normal phototactic behaviour.
tutlk14703/tutl23 mutants display a mild R7 tiling phenotype.
Class I ddaE neurons in tutl01085/tutl23 larvae have defects in their dendritic trees, including shortened interstitial branches and curled growth lacking directed orientation. There are also significantly more branch termini per neuron compared to wild type. The number of branch points on primary dendrites is unchanged compared to controls, but there is a clear increase in second and third order branch points in the mutant neurons. Homozygous and tutl23/Df(2L)ed-dp larvae show defects in the dendritic trees of class I ddaE neurons; there are significantly more branch termini per neuron compared to wild type, and although the number of branch points on primary dendrites is unchanged compared to controls, there is a clear increase in second and third order branch points in the mutant neurons. tutl23/+ larvae show defects in the dendritic trees of class I ddaE neurons; there are significantly more branch termini per neuron compared to wild type and there is an increase in second order branch points in the mutant neurons. The degree of branching is intermediate between that of homozygotes and wild type. Single cell homozygous ddaE neuron clones show an increase in branch termini comparable to that seen in homozygous animals, although other features of the homozygous mutant phenotype are not readily observed in the single cell mutant clones. Single cell homozygous clones of class II (ddaB) and class III (ddaA) neurons have a normal dendritic pattern. Single cell homozygous clones of class IV (ddaC) neurons show have a normal dendrite branch number. Class IV ddaC neurons in tutl01085/tutl23 larvae show numerous dendrite crossing points, in contrast to control neurons. Single cell homozygous clones of class IV (ddaC) neurons show numerous dendrite crossing points, in contrast to control neurons. tutl01085/tutl23 larvae show normal dendritic tiling among class IV da neurons (assayed by examining the borders between ddaC and v'ada neurons for dendritic overlap).
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
Statement
Reference
tutl23 has rhabdomere R7 phenotype, suppressible by bdlEX2
tutl23 has axon phenotype, suppressible by bdlEX2
tutl23 has rhabdomere R7 phenotype, suppressible by Df(2L)tutl-bdl/+
tutl23 has axon phenotype, suppressible by Df(2L)tutl-bdl/+
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
The R7 tiling pattern in homozygous tutl23, bdlEX2 mutants is normal. This effect appears to be R-cell-specific, as similar suppression is observed when bdl is selectively removed in homozygous tutl R cells by performing eye-specific mosaic analysis.
trc1/+ tutl23/+ double heterozygotes do not show any enhanced class I da neuron branching defects or class IV neuron dendrite self-avoidance defects compared to tutl23/+ single heterozygotes. There is no evidence for a genetic interaction between tutl23 and Dscam33 when assayed by studying dendrite self-avoidance in class IV da neurons.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Not rescued by
Comments
The defect in navigational response to gentle touch which is seen in homozygous tutl23 larvae is rescued by expression of tutlRE40452.Scer\UAS under the control of one of Scer\GAL4elav-C155, Scer\GAL4Appl.G1a or Scer\GAL4Cha.7.4. Expression under the control of one of Scer\GAL4VGlut-OK371, Scer\GAL4Ddc.PL, Scer\GAL4eve.RN2, Scer\GAL4G11-1, Scer\GAL4ftz.ng, Scer\GAL4D42, Scer\GAL4TrpA1.PR, Scer\GAL45-HTR1B, Scer\GAL4c81, Scer\GAL45-40, Scer\GAL4nompC.PC, Scer\GAL4iav.PK, Scer\GAL4pain-GAL4 or Scer\GAL4ppk.1.9 does not rescue the mutant phenotype. Expression of tutlRE40452.Scer\UAS under the control of Scer\GAL4elav-C155 from the beginning of the larval stage onwards is sufficient to rescue the defect in navigational response to gentle touch seen in homozygous tutl23 larvae, while limiting expression to the embryonic stage results in a failure of rescue (the temperature-sensitive Scer\GAL80ts.αTub84B allele has been used to limit the stage of expression).
Expression of tutlScer\UAS.cFa under the control of Scer\GAL4GMR.long rescues the mild tiling phenotype found in tutlk14703/tutl23 R7 terminals.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)