FlyBase Allele Report: Dmel\tutl[23]

General Information

Symbol

Dmel\tutl²³

Species

D. melanogaster

Name

FlyBase ID

FBal0241228

Feature type

allele

Associated gene

Dmel\tutl

Associated Insertion(s)

PBac{WHr}tutl²³

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Long et al., 2009)

Mutagen

FLPase

(Ferguson et al., 2009, Long et al., 2009)

Progenitor genotype

PBac{WH}tutl^f03096

(Ferguson et al., 2009, Long et al., 2009)

PBac{WH}tutl^f02770

(Ferguson et al., 2009, Long et al., 2009)

Associated Insertion(s)

PBac{WHr}tutl²³

Cytology

Description

Scer\FRT-mediate recombination between the two progenitor insertions has resulted in a deletion that removes amino acid residues Glu133 to Ala754 (the five Ig-like domains and the first fibronectin-type-III repeat) of tutl.

(Ferguson et al., 2009)

Recombination between the two progenitor insertions, resulting in a deletion of the intervening DNA between them and the reconstitution of the inserted element at the deletion site.

(Long et al., 2009)

Allele components

Component

Use(s)

Inserted element

PBac{WHr}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:4,296,031..4,312,800 [+]

Comment:

Deletion of part of tutl resulting from FLP-catalyzed recombination using the FRT-containing transposons PBac{WH}tutl^f03096 and PBac{WH}tutl^f02770.

(Ferguson et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon

(Cameron et al., 2013)

dendrite & dorsal multidendritic neuron ddaC (with tutl⁰¹⁰⁸⁵)

(Long et al., 2009)

dendrite & dorsal multidendritic neuron ddaC | somatic clone

(Long et al., 2009)

dendrite & dorsal multidendritic neuron ddaE

(Long et al., 2009)

dendrite & dorsal multidendritic neuron ddaE (with Df(2L)ed-dp)

(Long et al., 2009)

dendrite & dorsal multidendritic neuron ddaE (with tutl⁰¹⁰⁸⁵)

(Long et al., 2009)

dendrite & dorsal multidendritic neuron ddaE | somatic clone

(Long et al., 2009)

photoreceptor cell R7 (with tutl^k14703)

(Ferguson et al., 2009)

rhabdomere R7

(Cameron et al., 2013)

Detailed Description

Statement

Reference

Unlike in wild-type, many R7 terminals stick with neighbouring R7 terminals in homozygous tutl²³ mutants.

(Cameron et al., 2013)

Homozygous, tutl²³/tutl⁰¹⁰⁸⁵ and tutl²³/Df(2L)tutl larvae show a severe reduction compared to wild-type controls in changing moving direction in response to gentle touch at anterior segments. The number of exploratory head swings in response to the touch, and the time taken to select a new moving direction are increased. The withdrawal response to the gentle touch is normal.

Locomotory behavior in a stimulus-free condition is normal in homozygous and tutl²³/tutl⁰¹⁰⁸⁵ larvae (number of contractions, average speed, number of turns and average turning angles have been analysed).

Homozygous and tutl²³/tutl⁰¹⁰⁸⁵ larvae show normal phototactic behaviour.

(Zhou et al., 2012)

tutl^k14703/tutl²³ mutants display a mild R7 tiling phenotype.

(Ferguson et al., 2009)

Class I ddaE neurons in tutl⁰¹⁰⁸⁵/tutl²³ larvae have defects in their dendritic trees, including shortened interstitial branches and curled growth lacking directed orientation. There are also significantly more branch termini per neuron compared to wild type. The number of branch points on primary dendrites is unchanged compared to controls, but there is a clear increase in second and third order branch points in the mutant neurons.

Homozygous and tutl²³/Df(2L)ed-dp larvae show defects in the dendritic trees of class I ddaE neurons; there are significantly more branch termini per neuron compared to wild type, and although the number of branch points on primary dendrites is unchanged compared to controls, there is a clear increase in second and third order branch points in the mutant neurons.

tutl²³/+ larvae show defects in the dendritic trees of class I ddaE neurons; there are significantly more branch termini per neuron compared to wild type and there is an increase in second order branch points in the mutant neurons. The degree of branching is intermediate between that of homozygotes and wild type.

Single cell homozygous ddaE neuron clones show an increase in branch termini comparable to that seen in homozygous animals, although other features of the homozygous mutant phenotype are not readily observed in the single cell mutant clones.

Single cell homozygous clones of class II (ddaB) and class III (ddaA) neurons have a normal dendritic pattern.

Single cell homozygous clones of class IV (ddaC) neurons show have a normal dendrite branch number.

Class IV ddaC neurons in tutl⁰¹⁰⁸⁵/tutl²³ larvae show numerous dendrite crossing points, in contrast to control neurons.

Single cell homozygous clones of class IV (ddaC) neurons show numerous dendrite crossing points, in contrast to control neurons.

tutl⁰¹⁰⁸⁵/tutl²³ larvae show normal dendritic tiling among class IV da neurons (assayed by examining the borders between ddaC and v'ada neurons for dendritic overlap).

(Long et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tutl²³ has abnormal neuroanatomy phenotype, suppressible by bdl^EX2

(Cameron et al., 2013)

tutl²³ has abnormal neuroanatomy phenotype, suppressible by bdl^ΔIg1-2.UAS/bdl^EX2/Scer\GAL4^GMR.long

(Cameron et al., 2013)

tutl²³ has abnormal neuroanatomy phenotype, suppressible by Df(2L)tutl-bdl/+

(Cameron et al., 2013)

NOT suppressed by

Statement

Reference

tutl²³ has abnormal neuroanatomy phenotype, non-suppressible by bdl^UAS.Tag:FLAG/bdl^EX2/Scer\GAL4^GMR.long

(Cameron et al., 2013)

tutl²³ has abnormal neuroanatomy phenotype, non-suppressible by bdl^EX2/tutl^RE40452.UAS/Scer\GAL4^GMR.long

(Cameron et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

tutl²³ has rhabdomere R7 phenotype, suppressible by Df(2L)tutl-bdl/+

(Cameron et al., 2013)

tutl²³ has axon phenotype, suppressible by Df(2L)tutl-bdl/+

(Cameron et al., 2013)

tutl²³ has rhabdomere R7 phenotype, suppressible by bdl^EX2

(Cameron et al., 2013)

tutl²³ has axon phenotype, suppressible by bdl^EX2

(Cameron et al., 2013)

tutl²³ has rhabdomere R7 phenotype, suppressible by bdl^ΔIg1-2.UAS/bdl^EX2/Scer\GAL4^GMR.long

(Cameron et al., 2013)

tutl²³ has axon phenotype, suppressible by bdl^ΔIg1-2.UAS/bdl^EX2/Scer\GAL4^GMR.long

(Cameron et al., 2013)

NOT suppressed by

Statement

Reference

tutl²³ has rhabdomere R7 phenotype, non-suppressible by bdl^UAS.Tag:FLAG/bdl^EX2/Scer\GAL4^GMR.long

(Cameron et al., 2013)

tutl²³ has axon phenotype, non-suppressible by bdl^UAS.Tag:FLAG/bdl^EX2/Scer\GAL4^GMR.long

(Cameron et al., 2013)

tutl²³ has rhabdomere R7 phenotype, non-suppressible by bdl^EX2/tutl^RE40452.UAS/Scer\GAL4^GMR.long

(Cameron et al., 2013)

tutl²³ has axon phenotype, non-suppressible by bdl^EX2/tutl^RE40452.UAS/Scer\GAL4^GMR.long

(Cameron et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The R7 tiling pattern in homozygous tutl²³, bdl^EX2 mutants is normal. This effect appears to be R-cell-specific, as similar suppression is observed when bdl is selectively removed in homozygous tutl R cells by performing eye-specific mosaic analysis.

(Cameron et al., 2013)

trc¹/+ tutl²³/+ double heterozygotes do not show any enhanced class I da neuron branching defects or class IV neuron dendrite self-avoidance defects compared to tutl²³/+ single heterozygotes.

There is no evidence for a genetic interaction between tutl²³ and Dscam³³ when assayed by studying dendrite self-avoidance in class IV da neurons.

(Long et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tutl²³ is rescued by Scer\GAL4^elav-C155/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is rescued by Scer\GAL4^Appl.G1a/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is rescued by tutl^RE40452.UAS/Scer\GAL4^ChAT.7.4

(Zhou et al., 2012)

tutl²³/tutl^k14703 is rescued by tutl^RE40452.UAS/Scer\GAL4^GMR.long

(Ferguson et al., 2009)

tutl⁰¹⁰⁸⁵/tutl²³ is rescued by tutl^RE40452.UAS/Scer\GAL4²²¹

(Long et al., 2009)

tutl⁰¹⁰⁸⁵/tutl²³ is rescued by Scer\GAL4^elav-C155/tutl^RE40452.UAS

(Long et al., 2009)

tutl⁰¹⁰⁸⁵/tutl²³ is rescued by tutl^Δcyto.UAS/Scer\GAL4²²¹

(Long et al., 2009)

Partially rescued by

tutl⁰¹⁰⁸⁵/tutl²³ is partially rescued by Scer\GAL4^elav-C155/tutl^Δcyto.UAS

(Long et al., 2009)

Not rescued by

tutl²³ is not rescued by Scer\GAL4^VGlut1-OK371/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is not rescued by tutl^RE40452.UAS/Scer\GAL4^Ddc.PL

(Zhou et al., 2012)

tutl²³ is not rescued by Scer\GAL4^eve.RN2/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is not rescued by tutl^RE40452.UAS/Scer\GAL4^G11-1

(Zhou et al., 2012)

tutl²³ is not rescued by tutl^RE40452.UAS/Scer\GAL4^ftz.ng

(Zhou et al., 2012)

tutl²³ is not rescued by tutl^RE40452.UAS/Scer\GAL4^Toll-6-D42

(Zhou et al., 2012)

tutl²³ is not rescued by tutl^RE40452.UAS/Scer\GAL4^TrpA1.PR

(Zhou et al., 2012)

tutl²³ is not rescued by Scer\GAL4^5-HT1B.PY/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is not rescued by Scer\GAL4^c81/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is not rescued by Scer\GAL4^5-40/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is not rescued by Scer\GAL4^nompC.PC/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is not rescued by Scer\GAL4^iav.PK/tutl^RE40452.UAS

(Zhou et al., 2012)

tutl²³ is not rescued by tutl^RE40452.UAS/Scer\GAL4^pain-GAL4

(Zhou et al., 2012)

tutl²³ is not rescued by tutl^RE40452.UAS/Scer\GAL4^ppk.1.9

(Zhou et al., 2012)

Comments

The defect in navigational response to gentle touch which is seen in homozygous tutl²³ larvae is rescued by expression of tutl^{RE40452.Scer\UAS} under the control of one of Scer\GAL4^elav-C155, Scer\GAL4^Appl.G1a or Scer\GAL4^Cha.7.4. Expression under the control of one of Scer\GAL4^VGlut-OK371, Scer\GAL4^Ddc.PL, Scer\GAL4^eve.RN2, Scer\GAL4^G11-1, Scer\GAL4^ftz.ng, Scer\GAL4^D42, Scer\GAL4^TrpA1.PR, Scer\GAL4^5-HTR1B, Scer\GAL4^c81, Scer\GAL4^5-40, Scer\GAL4^nompC.PC, Scer\GAL4^iav.PK, Scer\GAL4^pain-GAL4 or Scer\GAL4^ppk.1.9 does not rescue the mutant phenotype.

Expression of tutl^{RE40452.Scer\UAS} under the control of Scer\GAL4^elav-C155 from the beginning of the larval stage onwards is sufficient to rescue the defect in navigational response to gentle touch seen in homozygous tutl²³ larvae, while limiting expression to the embryonic stage results in a failure of rescue (the temperature-sensitive Scer\GAL80^ts.αTub84B allele has been used to limit the stage of expression).

(Zhou et al., 2012)

Expression of tutl^Scer\UAS.cFa under the control of Scer\GAL4^GMR.long rescues the mild tiling phenotype found in tutl^k14703/tutl²³ R7 terminals.

(Ferguson et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tutl²³

(Chen et al., 2017, Cameron et al., 2013, Zhou et al., 2012, Sulkowski et al., 2011, Ferguson et al., 2009, Long et al., 2009)

Δtutl

(Chen et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (6)

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