Scer\FRT-mediate recombination between the two progenitor insertions has resulted in a deletion that removes amino acid residues Glu133 to Ala754 (the five Ig-like domains and the first fibronectin-type-III repeat) of tutl.
Recombination between the two progenitor insertions, resulting in a deletion of the intervening DNA between them and the reconstitution of the inserted element at the deletion site.
dendrite & dorsal multidendritic neuron ddaC | somatic clone
dendrite & dorsal multidendritic neuron ddaE
dendrite & dorsal multidendritic neuron ddaE | somatic clone
The R7 tiling pattern in homozygous tutl23, bdlEX2 mutants is normal. This effect appears to be R-cell-specific, as similar suppression is observed when bdl is selectively removed in homozygous tutl R cells by performing eye-specific mosaic analysis.
The defect in navigational response to gentle touch which is seen in homozygous tutl23 larvae is rescued by expression of tutlRE40452.Scer\UAS under the control of one of Scer\GAL4elav-C155, Scer\GAL4Appl.G1a or Scer\GAL4Cha.7.4. Expression under the control of one of Scer\GAL4VGlut-OK371, Scer\GAL4Ddc.PL, Scer\GAL4eve.RN2, Scer\GAL4G11-1, Scer\GAL4ftz.ng, Scer\GAL4D42, Scer\GAL4TrpA1.PR, Scer\GAL45-HTR1B, Scer\GAL4c81, Scer\GAL45-40, Scer\GAL4nompC.PC, Scer\GAL4iav.PK, Scer\GAL4pain-GAL4 or Scer\GAL4ppk.1.9 does not rescue the mutant phenotype.
Expression of tutlRE40452.Scer\UAS under the control of Scer\GAL4elav-C155 from the beginning of the larval stage onwards is sufficient to rescue the defect in navigational response to gentle touch seen in homozygous tutl23 larvae, while limiting expression to the embryonic stage results in a failure of rescue (the temperature-sensitive Scer\GAL80ts.αTub84B allele has been used to limit the stage of expression).