FB2025_01 , released February 20, 2025
Allele: Scer\GAL4Ilp3.PB
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General Information
Symbol
Scer\GAL4Ilp3.PB
Species
S. cerevisiae
Name
Insulin-like peptide 3 promoter construct of Buch
FlyBase ID
FBal0241232
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Ilp3-Gal4.B}
Also Known As
Dilp3-GAL4, InsP3-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Buch et al., 2008)
Progenitor genotype
Carried in construct
P{Ilp3-Gal4.B}
Cytology
Description

An 860bp Ilp3 promoter fragment regulates expression of a GAL4 driver.

(Buch et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
Ilp3
Ilp3_InsP3-Gal4
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      larval insulin secreting cell of pars intercerebralis
      (Buch et al., 2008)
      adult stage
      adult insulin secreting cell of pars intercerebralis
      (Slack et al., 2011, Buch et al., 2008)
      Additional Information
      Statement
      Reference

      ScerGAL4Ilp3.PB drives expression in dorsomedial neurosecretory cells (mNSCs).

      (Slack et al., 2011)
       
      Marker for
      Reflects expression of
      Ilp3
      (Slack et al., 2011, Buch et al., 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ablation of Scer\GAL4Ilp3.PB-expressing median neurosecretory cells (mNSCs) extends lifespan of mated female flies compared to controls when flies are fed either 1x or 8x the optimal amount of sucrose. Ablated flies climb better than wild type flies with age, and this increase is unaffected by excess sugar intake. The increase in storage triacylglycerol (TAG) levels seen in wild type flies fed 8x the optimal amount of sugar is not observed in ablated flies: TAG levels are similar when flies are fed either 1x and 8x sugar.

      A foxoΔ94 mutant background is unable to restore the normal sugar-induced increase in triacylglycerol levels that is seen upon ablation of the Scer\GAL4Ilp3.PB-expressing median neurosecretory cells (mNSCs).

      (Al Saud et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Scer\GAL4Ilp3.PB, rprUAS.C has long lived phenotype, suppressible by foxoΔ94

      (Slack et al., 2011)

      Scer\GAL4Ilp3.PB, rprUAS.C has long lived | drug conditional phenotype, suppressible | partially by foxoΔ94

      (Slack et al., 2011)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4Ilp3.PB, rprUAS.C has decreased body size phenotype, non-suppressible by foxoΔ94

      (Slack et al., 2011)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4Ilp3.PB/ShEKO.UAS.GFP(GL) is a non-suppressor of decreased body size | adult stage phenotype of upd2Δ3-62

      (Rajan and Perrimon, 2012)

      rprUAS.C/Scer\GAL4Ilp3.PB is a non-suppressor of viable phenotype of foxoΔ94

      (Slack et al., 2011)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Scer\GAL4Ilp3.PB/Bhal\NaChBacUAS.EGFP is a suppressor of wing phenotype of upd2Δ3-62

      (Rajan and Perrimon, 2012)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4Ilp3.PB/ShEKO.UAS.GFP(GL) is a non-suppressor of wing phenotype of upd2Δ3-62

      (Rajan and Perrimon, 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4Ilp3.PB
      Name Synonyms
      Insulin-like peptide 3 promoter construct of Buch
      Secondary FlyBase IDs
        References (15)