FlyBase Allele Report: Dmel\Ubqn[dsRNA.Ex2.UAS]

General Information

Symbol

Dmel\Ubqn^{dsRNA.Ex2.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0241241

Feature type

allele

Associated gene

Dmel\Ubqn

Associated Insertion(s)

Carried in Construct

P{UAS-Ubqn.Ex2-RNAi}

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Li et al., 2007)

in vitro construct - RNAi

(Li et al., 2007)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-Ubqn.Ex2-RNAi}

Cytology

Nature of the lesion

Statement

Reference

UAS regulatory sequences drive expression of inverted repeat sequences corresponding to exon 2 of Ubqn (a 545bp fragment).

(Li et al., 2007)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ubqn

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Psn^+14.UAS

(Li et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | male limited, with Scer\GAL4^elav-C155

(Li et al., 2007)

increased cell death | male limited, with Scer\GAL4^elav-C155

(Li et al., 2007)

partially lethal - majority die | male limited, with Scer\GAL4^sd-SG29.1

(Li et al., 2007)

short lived | male limited, with Scer\GAL4^elav-C155

(Li et al., 2007)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^e22c

(Li et al., 2007)

anterior crossvein, with Scer\GAL4^ptc-559.1

(Li et al., 2007)

anterior crossvein, with Scer\GAL4^sd-SG29.1

(Li et al., 2007)

neuropil, with Scer\GAL4^elav-C155

(Li et al., 2007)

posterior crossvein, with Scer\GAL4^e22c

(Li et al., 2007)

posterior crossvein, with Scer\GAL4^sd-SG29.1

(Li et al., 2007)

wing cell, with Scer\GAL4^ptc-559.1

(Li et al., 2007)

wing margin, with Scer\GAL4^sd-SG29.1

(Li et al., 2007)

wing vein L3, with Scer\GAL4^e22c

(Li et al., 2007)

wing vein L3, with Scer\GAL4^sd-SG29.1

(Li et al., 2007)

wing vein L4, with Scer\GAL4^e22c

(Li et al., 2007)

wing vein L4, with Scer\GAL4^sd-SG29.1

(Li et al., 2007)

wing vein L5, with Scer\GAL4^e22c

(Li et al., 2007)

wing vein L5, with Scer\GAL4^sd-SG29.1

(Li et al., 2007)

Detailed Description

Statement

Reference

Male flies silencing Ubqn through expression of Ubqn^{dsRNA.Ex2.Scer\UAS} in neurons under the control of Scer\GAL4^elav-C155 suffer premature death. The flies are born normally, but life-span begins to decline shortly after eclosion and significantly declines by the time the flies are 30 days old. At 50 days, over 70% of control flies are still alive, whereas over 80% of Ubqn^{dsRNA.Ex2.Scer\UAS};Scer\GAL4^elav-C155 flies are already dead. In contrast, no significant difference in life-span is found in mutant females compared to wild-type.

Ubqn^{dsRNA.Ex2.Scer\UAS};Scer\GAL4^elav-C155 male flies exhibit severe widespread neurodegeneration compared with wild-type flies. In addition, multilamellar structures are found throughout the brain, and the neuropil contains multiple membrane-bound vacuoles.

Silencing of Ubqn by expression of Ubqn^{dsRNA.Ex2.Scer\UAS} under the control of Scer\GAL4^sd-SG29.1 in wing discs results in three types of phenotypes: (i) loss of the partial L4, L5, entire anterior cross vein and posterior cross vein; (ii) a thickened L3 vein, particularly in the distal portion of the margin area of the wing; (iii) notches in the wing margin. These phenotypes show variable penetrance in male and female flies. Compared with female flies, male Ubqn^{dsRNA.Ex2.Scer\UAS};Scer\GAL4^elav-C155 flies are semi-lethal. All the male flies and approximately 5% of female flies exhibit all three severe phenotypes. The majority of the female flies (95%) exhibit only a weak phenotype as evidenced by loss of the posterior cross vein and anterior cross vein.

Silencing of Ubqn by expression of Ubqn^{dsRNA.Ex2.Scer\UAS} in the wing disc under the control of Scer\GAL4^e22c leads to loss of the entire posterior cross vein, partial loss of the anterior cross vein, and L5 veins and weakened distal portion of L4 and L3-4 M veins in the posterior portion of the wing.

Silencing of Ubqn by expression of Ubqn^{dsRNA.Ex2.Scer\UAS} in the wing disc under the control of Scer\GAL4^ptc-559.1 results in loss of the entire anterior cross vein, narrowing of the intervein sector between L3 and L4 veins in all flies, compared with wild-type controls.

Silencing of Ubqn by expression of Ubqn^{dsRNA.Ex2.Scer\UAS} in the developing eye disc under the control of Scer\GAL4^GMR.PF does not lead to any detectable eye abnormalities.

(Li et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF, Ubqn^{dsRNA.Ex2.UAS} is an enhancer of increased cell death phenotype of Psn^+14.UAS

(Li et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^e22c, Ubqn^{dsRNA.Ex2.UAS} has wing vein L4 phenotype, suppressible | partially by rho^ve-1/Scer\GAL4^e22c

(Li et al., 2007)

Scer\GAL4^e22c, Ubqn^{dsRNA.Ex2.UAS} has wing vein L5 phenotype, suppressible | partially by rho^ve-1/Scer\GAL4^e22c

(Li et al., 2007)

Scer\GAL4^e22c, Ubqn^{dsRNA.Ex2.UAS} has wing vein L3 phenotype, suppressible | partially by rho^ve-1/Scer\GAL4^e22c

(Li et al., 2007)

Scer\GAL4^e22c, Ubqn^{dsRNA.Ex2.UAS} has posterior crossvein phenotype, suppressible | partially by rho^ve-1/Scer\GAL4^e22c

(Li et al., 2007)

Enhancer of

Statement

Reference

Ubqn^{dsRNA.Ex2.UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Psn^+14.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Li et al., 2007)

Ubqn^{dsRNA.Ex2.UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Psn^+14.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Li et al., 2007)

Ubqn^{dsRNA.Ex2.UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Psn^+14.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Li et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Ubqn^{dsRNA.Ex2.Scer\UAS} by Scer\GAL4^e22c in a heterozygous rho^ve-1 background leads to a normal anterior cross vein and partially restored posterior cross vein and L5 vein, while the distal portion of L4 and L3-4 M veins corresponding to the posterior portion of the wing remains weakened, compared with Ubqn^{dsRNA.Ex2.Scer\UAS}; Scer\GAL4^e22c single mutants.

Silencing of Ubqn through co-expression of Ubqn^{dsRNA.Ex2.Scer\UAS} with Psn^+14.Scer\UAS in the developing eye under the control of Scer\GAL4^GMR.PF enhances the Psn^+14.Scer\UAS-induced rough eye phenotype, leading to an even smaller and rougher eye than that observed with Psn^+14.Scer\UAS expression alone. Double mutants exhibit a loss of eye pigmentation, suggesting the loss of pigment cells. RNAi silencing of Ubqn in addition to Psn^+14.Scer\UAS overexpression results in a much more severe degenerative phenotype than Psn^+14.Scer\UAS single mutants, as evidenced by numerous visible holes (2-5υm in diameter) on a small, rough eye. In addition, the ommatidia are very irregular and mostly fused with very few bristles.

(Li et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

90858

y¹ w^*; P{UAS-Ubqn.Ex2-RNAi}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ubqn^{dsRNA.Ex2.Scer\UAS}

Ubqn^{dsRNA.Ex2.UAS}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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