FlyBase Allele Report: Dmel\ZnT63C[RNAi.UAS.cWa]

General Information

Symbol

Dmel\ZnT63C^RNAi.UAS.cWa

Species

D. melanogaster

Name

FlyBase ID

FBal0241248

Feature type

allele

Associated gene

Dmel\ZnT63C

Associated Insertion(s)

Carried in Construct

P{UAS-ZnT63C.RNAi.W}

Key Links

Transgenic product class

RNAi_reagent

(Wang et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wang et al., 2009)

Mutagen

in vitro construct

(Wang et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-ZnT63C.RNAi.W}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat corresponding to positions 619-1026 (GenBank accession no. NM_168017) in the coding region of ZnT63C. The 408 bp DNA fragment extends from exon 3 to the splice acceptor of intron 5 and it is common in all splice variants of ZnT63C.

(Wang et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ZnT63C

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

No obvious eye phenotypes are seen when ZnT63C^{dsRNA.Scer\UAS.cWa} is expressed under the control of Scer\GAL4^elav.PU.

(Huang et al., 2014)

On normal food, ubiquitous expression of ZnT63C^{dsRNA.Scer\UAS.cWa} by Scer\GAL4^da.G32 does not cause any abnormalities in viability, morphology, or fertility.

When grown on food supplemented with the zinc chelator ethylenediamine-tetraacetic acid (EDTA), larvae expressing Scer\GAL4^da.G32-driven ZnT63C^{dsRNA.Scer\UAS.cWa} exhibit extremely retarded development. After 6-7 days of feeding and growth on EDTA-supplemented medium, control larvae reach wandering stage. In contrast, ZnT63C^{dsRNA.Scer\UAS.cWa} expressing larvae are much smaller in size, and their anatomical characteristics of mouth hooks and apical spiracles are similar to those of the second instar larvae. These larvae continue to grow and can survive as long as 20-25 days. However, they fail to pupate and ultimately die at the third-instar stage. The phenotype is chelator dosage sensitive, i.e., lower concentrations of EDTA allow escapers to survive until pupal stage and even some to viable adulthood. The ZnT63C^{dsRNA.Scer\UAS.cWa} expressing larvae are also sensitive to another membrane-permeable zinc chelator, TPEN, but not to the copper-specific chelator bathocuproine-disulfonate or the iron specific chelator bathophenanthroline-disulfonate.

Ubiquitous expression of ZnT63C^{dsRNA.Scer\UAS.cWa} by Scer\GAL4^Act5C.PI engenders impaired development of larvae to adults under dietary zinc limitation. Adding zinc back into the EDTA-containing medium restores the survival of the ZnT63C^{dsRNA.Scer\UAS.cWa} expressing larvae to nearly normal level. None of the other tested metals, including iron, copper, and manganese has any ameliorating effect, indicating that the lethality is due specifically to zinc deficiency.

On normal food, the zinc content of larvae expressing Scer\GAL4^da.G32-driven ZnT63C^{dsRNA.Scer\UAS.cWa} does not differ significantly from wild-type controls. In the presence of EDTA in the medium, ZnT63C^{dsRNA.Scer\UAS.cWa} expressing larvae accumulate a lower total body zinc content. Iron content does not significantly differ in wild-type and ZnT63C^{dsRNA.Scer\UAS.cWa} expressing larvae under either normal or EDTA-supplemented conditions.

Expression of ZnT63C^{dsRNA.Scer\UAS.cWa} driven by either Scer\GAL4^NP3084 or Scer\GAL4^NP5021 in the gut mimics the effects of ubiquitous inhibition of ZnT63C by RNAi as measured by survival rates.

Flies expressing ZnT63C^{dsRNA.Scer\UAS.cWa} driven by one of the drivers Scer\GAL4^elav.PLu, Scer\GAL4^how-24B, Scer\GAL4^{CG10962-NP1093}, Scer\GAL4^NP403 or Scer\GAL4^ninaD.PW appear phenotypically normal.

(Wang et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^da.G32, ZnT63C^RNAi.UAS.cWa has lethal | third instar larval stage phenotype, suppressible by Hsap\SLC30A1^UAS.cWa, Scer\GAL4^da.G32

(Wang et al., 2009)

Scer\GAL4^NP3084, ZnT63C^RNAi.UAS.cWa has lethal | larval stage phenotype, suppressible by Hsap\SLC30A1^UAS.cWa, Scer\GAL4^NP3084

(Wang et al., 2009)

Scer\GAL4^unspecified, ZnT63C^RNAi.UAS.cWa has lethal | larval stage phenotype, suppressible by Hsap\SLC30A1^UAS.EGFP, Scer\GAL4^unspecified

(Wang et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.G32, ZnT63C^RNAi.UAS.cWa has lethal | larval stage phenotype, non-suppressible by Hsap\SLC30A7^UAS.cWa, Scer\GAL4^da.G32

(Wang et al., 2009)

Scer\GAL4^NP3084, ZnT63C^RNAi.UAS.cWa has lethal | larval stage phenotype, non-suppressible by Hsap\SLC30A7^UAS.cWa, Scer\GAL4^NP3084

(Wang et al., 2009)

Scer\GAL4^unspecified, ZnT63C^RNAi.UAS.cWa has lethal | larval stage phenotype, non-suppressible by Hsap\SLC30A4^UAS.cWa, Scer\GAL4^unspecified

(Wang et al., 2009)

Enhancer of

Statement

Reference

ZnT63C^RNAi.UAS.cWa, Scer\GAL4^elav.PU is an enhancer of visible phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Huang et al., 2014)

ZnT63C^RNAi.UAS.cWa, Scer\GAL4^elav.PU is an enhancer of short lived phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Huang et al., 2014)

ZnT63C^RNAi.UAS.cWa, Scer\GAL4^elav.PU is an enhancer of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Huang et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of ZnT63C^{dsRNA.Scer\UAS.cWa} enhances the rough eye phenotype seen in flies expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PU. Lifespan is also further shortened. The level of vacuolisation in the CNS is exacerbated.

(Huang et al., 2014)

Co-expression of Hsap\SLC30A1^Scer\UAS.cWa with ZnT63C^{dsRNA.Scer\UAS.cWa} driven by either Scer\GAL4^da.G32 or Scer\GAL4^NP3084 restores the eclosion rates of larvae reared on EDTA supplemented food.

Co-expression of Hsap\SLC30A7^Scer\UAS.cWa with ZnT63C^{dsRNA.Scer\UAS.cWa} driven by either Scer\GAL4^da.G32 or Scer\GAL4^NP3084 does not alleviate the lethality of ZnT63C silencing by RNAi on EDTA supplemented food.

Co-expression of Hsap\SLC30A4^Scer\UAS.cWa with ZnT63C^{dsRNA.Scer\UAS.cWa} driven by Scer\GAL4^unspecified does not alleviate the lethality of ZnT63C silencing by RNAi on EDTA supplemented food.

Co-expression of Hsap\SLC30A1^{Scer\UAS.T:Avic\GFP-EGFP} with ZnT63C^{dsRNA.Scer\UAS.cWa} driven by Scer\GAL4^unspecified restores the eclosion rates of larvae reared on EDTA supplemented food.

(Wang et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression of ZnT63C^{dsRNA.Scer\UAS.cWa} produces dsRNA which results in dsRNA interference (RNAi) of the ZnT63C gene.

(Wang et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ZnT63C^RNAi.UAS.cWa

ZnT63C^{dsRNA.Scer\UAS.cWa}

ZnT63C^{dsRNA.UAS.cWa}

Name Synonyms

Secondary FlyBase IDs

References (4)

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