FlyBase Allele Report: Hsap\UBE3A[UAS.cRa]

General Information

Symbol

Hsap\UBE3A^UAS.cRa

Species

H. sapiens

Name

FlyBase ID

FBal0241351

Feature type

allele

Associated gene

Hsap\UBE3A

Associated Insertion(s)

Carried in Construct

P{UAS-hUBE3A.R}

Also Known As

UAS-hUBE3A, UAS-UBE3A

Key Links

Transgenic product class

wild_type

(Reiter et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Reiter et al., 2006)

Mutagen

in vitro construct

(Reiter et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-hUBE3A.R}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\UBE3A full-length coding sequences.

(Reiter et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\UBE3A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

DOES NOT model cervical cancer

CEA with HPV18\E6^UAS.Tag:MYC

(Padash Barmchi et al., 2016)

model of cervical cancer

CEA with HPV18\E6^UAS.Tag:MYC, InR^DN.UAS.cUa

(Padash Barmchi et al., 2016)

CEA with HPV18\E6^UAS.Tag:MYC, Ras85D^V12.cUa.UAS

(Padash Barmchi et al., 2016)

CEA with HPV18\E6^{V158A.UAS.Tag:MYC}, Ras85D^V12.cUa.UAS

(Padash Barmchi et al., 2016)

CEA with HPV18\E6^UAS.Tag:MYC, N^int.U.UAS

(Padash Barmchi et al., 2016)

model of carcinoma

CEA with HPV16\E5^UAS.Tag:HA

(Hashemi et al., 2022)

CEA with HPV16\E6^UAS.cHa

(Hashemi et al., 2022)

CEA with HPV16\E7^UAS.cHa

(Hashemi et al., 2022)

CEA with HPV18\E6^UAS.Tag:MYC

(Padash Barmchi et al., 2021)

CEA with HPV18\E6^UAS.Tag:MYC, Ras64B^Val14.UAS

(Padash Barmchi et al., 2021)

model of cancer

CEA

(Collins et al., 2023)

CEA with HPV18\E6^UAS.Tag:MYC

(Collins et al., 2023)

model of epilepsy

CEA

(Hope et al., 2017)

model of Human papillomavirus infectious disease

CEA with HPV18\E6^UAS.Tag:MYC

(Padash Barmchi et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4²²¹

(Lu et al., 2009)

abnormal neurophysiology | adult stage, with Scer\GAL4^repo

(Hope et al., 2017)

bang sensitive | adult stage | progressive, with Scer\GAL4^repo

(Hope et al., 2017)

paralytic | adult stage | heat sensitive, with Scer\GAL4^repo

(Hope et al., 2017)

Phenotype Manifest In

larval dorsal multidendritic neuron ddaE, with Scer\GAL4²²¹

(Lu et al., 2009)

larval dorsal multidendritic neuron ddaF, with Scer\GAL4²²¹

(Lu et al., 2009)

Detailed Description

Statement

Reference

The expression of Hsap\UBE3A^Scer\UAS.cRa under the control of Scer\GAL4^repo leads to adults showing progressive bang-induced paralysis and heat-induced paralysis, but not photogenic paralysis. This expression also leads significant decreases in the amplitudes of On transients, Off transients and photoreceptor potential in electroretinograms, as compared to controls.

(Hope et al., 2017)

The expression of Hsap\UBE3A^Scer\UAS.cRa under the control of either Scer\GAL4^ap.PU or Scer\GAL4^GMR.PU does not lead to obvious wing or eye defect, as compared to controls.

(Padash Barmchi et al., 2016)

Expression of Hsap\UBE3A^Scer\UAS.cRa in a subset of DA sensory neurons using Scer\GAL4²²¹ causes decreased dendritic branching of ddaE and ddaF neurons.

(Lu et al., 2009)

Expression of Hsap\UBE3A^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PF at 25[o]C has no discernable effect on eye development.

(Reiter et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by MED19^JF02713, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Prosβ2^1.UAS, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by mop^HM05008, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by mop^HMS00706, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by tara^JF01421, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by sqz^UAS.cAa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Trhn^UAS.cYa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by nmo^JF01799, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Vha100-1^JF02059, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by pum^HMS01564, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by pum^JF02267, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Rtf1^HM04026, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Rtf1^HMS06074, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Tao^GL00015, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Tao^HMS02333, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by InR^UAS.cUa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by InR^DN.UAS.cUa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

NOT Enhanced by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by yki^{UAS.EGFP,Tag:HA}, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by hh[+]/hh^AC

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by wit^A12/wit[+]

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by bsk^DN.UAS, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Tak1[+]/Tak1²⁵²⁷

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Buffy[+]/Buffy^H37

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Debcl[+]/Debcl^E26

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Debcl^UAS.cUa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by hop²/hop[+]

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by rl^Sem.cUa.UAS, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Akt1[+]/Akt⁰⁴²²⁶

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by arm^{S10.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Suppressed by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by α-Cat^UAS.sgGFP, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by CG10631^HMC06634, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has increased cell death | third instar larval stage phenotype, suppressible by Magi^UAS.mCherry, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has increased cell death | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has visible | adult stage phenotype, suppressible | partially by Magi^UAS.mCherry, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has visible | adult stage phenotype, suppressible | partially by Magi^{ΔWW.UAS.Tag:FLAG}, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

NOT suppressed by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by p53^H159N.UAS.Ex, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has increased cell death | third instar larval stage phenotype, non-suppressible by bsk^DN.UAS, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has increased cell death | third instar larval stage phenotype, non-suppressible by scrib^UASp.GFP, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has visible | adult stage phenotype, non-suppressible by Magi^{ΔPDZ.UAS.Tag:FLAG}, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has visible | adult stage phenotype, non-suppressible by scrib^UASp.GFP, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has visible | adult stage phenotype, non-suppressible by dlg1^UAS.EGFP, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PU, Hsap\UBE3A^UAS.cRa/HPV18\E6^UAS.Tag:MYC is a non-suppressor of lethal - all die before end of pupal stage phenotype of Ras85D^V12.cUa.UAS, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Other

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU, mop^HM05008 has melanotic necrosis | adult stage phenotype

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU, mop^HMS00706 has melanotic necrosis | adult stage phenotype

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has visible | adult stage phenotype

(Collins et al., 2023, Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has visible | adult stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Ras85D^V12.cUa.UAS, Scer\GAL4^GMR.PU has abnormal cell migration | pupal stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, N^int.U.UAS, Scer\GAL4^GMR.PU has abnormal cell migration | pupal stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, N^int.U.UAS, Scer\GAL4^GMR.PU has lethal - all die before end of P-stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, InR^UAS.cUa, Scer\GAL4^GMR.PU has neoplasia phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has abnormal cell adhesion | adult stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has increased cell death | third instar larval stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^{V158A.UAS.Tag:MYC}, Hsap\UBE3A^UAS.cRa, Ras85D^V12.cUa.UAS, Scer\GAL4^GMR.PU has abnormal cell migration | pupal stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Ras85D^V12.cUa.UAS, Scer\GAL4^ap.PU has abnormal cell shape | third instar larval stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Ras85D^V12.cUa.UAS, Scer\GAL4^ap.PU has abnormal cell adhesion | third instar larval stage phenotype

(Padash Barmchi et al., 2016)

BacA\p35^UAS.cHa, HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has abnormal cell adhesion | third instar larval stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Magi^bst, Scer\GAL4^ap.PU has lethal - all die before end of pupal stage phenotype

(Padash Barmchi et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by MED19^JF02713, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Prosβ2^1.UAS, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by mop^HM05008, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by mop^HMS00706, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by tara^JF01421, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by sqz^UAS.cAa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Trhn^UAS.cYa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by nmo^JF01799, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Vha100-1^JF02059, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by pum^HMS01564, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by pum^JF02267, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Rtf1^HM04026, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Rtf1^HMS06074, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Tao^GL00015, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Tao^HMS02333, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by InR^UAS.cUa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by InR^DN.UAS.cUa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

NOT Enhanced by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by yki^{UAS.EGFP,Tag:HA}, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by hh[+]/hh^AC

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by wit^A12/wit[+]

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by bsk^DN.UAS, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Tak1[+]/Tak1²⁵²⁷

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Buffy[+]/Buffy^H37

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Debcl[+]/Debcl^E26

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Debcl^UAS.cUa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by hop²/hop[+]

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by rl^Sem.cUa.UAS, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Akt1[+]/Akt⁰⁴²²⁶

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Ras85D^N17.UAS, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by arm^{S10.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Suppressed by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by α-Cat^UAS.sgGFP, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by CG10631^HMC06634, Scer\GAL4^GMR.PU

(Collins et al., 2023)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing disc dorsal compartment | third instar larval stage phenotype, suppressible by Magi^UAS.mCherry, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing phenotype, suppressible | partially by Magi^UAS.mCherry, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing phenotype, suppressible | partially by Magi^{ΔWW.UAS.Tag:FLAG}, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

NOT suppressed by

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing disc dorsal compartment | third instar larval stage phenotype, non-suppressible by bsk^DN.UAS, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing disc dorsal compartment | third instar larval stage phenotype, non-suppressible by scrib^UASp.GFP, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by p53^H159N.UAS.Ex, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing phenotype, non-suppressible by Magi^{ΔPDZ.UAS.Tag:FLAG}, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing phenotype, non-suppressible by scrib^UASp.GFP, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing phenotype, non-suppressible by dlg1^UAS.EGFP, Scer\GAL4^ap.PU

(Padash Barmchi et al., 2016)

Suppressor of

Statement

Reference

Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415

(Reiter et al., 2006)

Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415

(Reiter et al., 2006)

Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, faf^EP381

(Reiter et al., 2006)

Other

Statement

Reference

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has eye phenotype

(Collins et al., 2023, Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has ommatidium phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU has interommatidial bristle | increased number phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^{V158A.UAS.Tag:MYC}, Hsap\UBE3A^UAS.cRa, Ras85D^V12.cUa.UAS, Scer\GAL4^ap.PU has imaginal disc epithelial cell | pupal stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^ap.PU has wing disc dorsal compartment | third instar larval stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Ras85D^V12.cUa.UAS, Scer\GAL4^ap.PU has imaginal disc epithelial cell | pupal stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, N^int.U.UAS, Scer\GAL4^ap.PU has imaginal disc epithelial cell | pupal stage phenotype

(Padash Barmchi et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of Hsap\UBE3A^UAS.cRa and HPV18\E6^UAS.Tag:MYC under the control of Scer\GAL4^ap.PU in a Magi^bst-homozygous background leads to pupal lethality.

The co-expression at room temperature of Hsap\UBE3A^UAS.cRa and HPV18\E6^UAS.Tag:MYC under the control of Scer\GAL4^ap.PU leads to fully penetrant adult wing defects, including smaller and blistered held-out wings that are full of melanized tissue, while co-expression at 29[o]C leads to increased apoptosis in the dorsal compartment of third instar larval wing discs (assessed by the number of Casp3-positive cells), as compared to controls; the severity of wing defects is ameliorated by additional co-expression of Magi^UAS.mCherry or Magi^{ΔWW.UAS.Tag:FLAG}, but not of Magi^{ΔPDZ.UAS.Tag:FLAG}, scrib^UASp.GFP or dlg1^UAS.EGFP, while the third instar larval wing disc defects are rescued by the additional co-expression of Magi^UAS.mCherry but not of scrib^UASp.GFP or bsk^DN.UAS; additional co-expression of BacA\p35^UAS.cHa also rescues the increased apoptosis at the dorsal compartment of third instar larval wing discs without leading to disc overgrowth, but induces basal delamination of cell clusters, as compared to controls.

The triple co-expression of Hsap\UBE3A^UAS.cRa, HPV18\E6^UAS.Tag:MYC and Ras85D^V12.cUa.UAS under the control of Scer\GAL4^ap.PU from the first or second instar larval stage onwards (by the use of Gal80[ts]) results in the most highly expressing cells within the dorsal compartment of the third instar larval imaginal disc adopting a fibroblast-like and flat morphology, displaying filopodial-like processes and delaminating at the basal side of the columnar epithelium, although without crossing the dorsal-ventral boundary, as compared to controls.

The co-expression of Hsap\UBE3A^UAS.cRa and HPV18\E6^UAS.Tag:MYC under the control of Scer\GAL4^GMR.PU leads to a fully penetrant rough eye phenotype, disorganized and fused ommatidia (resulting in increased numbers of cone cells, bristle cells and primary, secondary and tertiary pigment cells per ommatidium), and abnormal adherens junctions between photoreceptors, as compared to the single expressions or wild-type controls; this rough eye phenotype is enhanced by the co-expression of either InR^UAS.cUa, leading to overgrowth and out-pocketing of the eye tissue, or InR^DN.UAS.cUa, leading to severe eye necrosis, but is not modified by heterozygosity for Akt1⁰⁴²²⁶, hh^AC, wit^A12, Tak1²⁵²⁷, Buffy^H37, Debcl^E26, hop² or Stat92E⁰⁶³⁴⁶, or by the additional co-expression of rl^Sem.cUa.UAS, arm^{S10.UAS.Tag:MYC}, yki^{UAS.EGFP,Tag:HA}, bsk^DN.UAS, Debcl^UAS.cUa or p53^H159N.UAS.Ex.

The triple co-expression of Hsap\UBE3A^UAS.cRa, HPV18\E6^UAS.Tag:MYC and either Ras85D^V12.cUa.UAS or N^int.U.UAS under the control of Scer\GAL4^GMR.PU leads to the migration of a considerable proportion of expressing eye tissue cells into distant pupal tissues (e.g. abdomen), as compared to controls; the N^int.U.UAS triple co-expression condition also leads to pharate lethality, while the Ras85D^V12.cUa.UAS triple co-expression condition does not suppress the pupal lethality induced by the single expression of Ras85D^V12.cUa.UAS. The triple co-expression of Hsap\UBE3A^UAS.cRa and the different HPV18\E6^{V158A.UAS.Tag:MYC} allele with Ras85D^V12.cUa.UAS, but not N^int.U.UAS, under the control of Scer\GAL4^GMR.PU leads to the migration of a considerable proportion of the expressing eye cells into distant pupal tissues (e.g. abdomen), as compared to controls.

(Padash Barmchi et al., 2016)

Co-expression of Hsap\UBE3A^Scer\UAS.cRa and pbl^EP3415 under the control of Scer\GAL4^GMR.PF at 25[o]C rescues much of the effect of pbl^EP3415 overexpression. Although eyes still have a mild rough appearance, fewer than 3% exhibit necrotic foci and the ommatidia are much better organised and well formed than in pbl^EP3415 (Scer\GAL4^GMR.PF) flies, including six to seven photoreceptor cells per ommatidium.

The synergistic effect of pbl^EP3415 and faf^EP381 can be overcome by co-expression with Hsap\UBE3A^Scer\UAS.cRa (all under the control of Scer\GAL4^GMR.PF.

(Reiter et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

90373

w^*; P{UAS-hUBE3A.R}77L

90372

w^*; P{UAS-hUBE3A.R}31/CyO