Allele Hsap\UBE3A^Scer\UAS.cRa

General Information
Symbol	Hsap\UBE3A^Scer\UAS.cRa	Species	H. sapiens
Name		FlyBase ID	FBal0241351
Feature type	allele	Associated gene	Hsap\UBE3A

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Reiter et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\UBE3A full-length coding sequences. (Reiter et al., 2006)
Carried in construct	P{UAS-hUBE3A.R} (Reiter et al., 2006, Lu et al., 2009, Ferdousy et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4²²¹ (Lu et al., 2009)
Phenotype Manifest In
	dorsal multidendritic neuron ddaE, with Scer\GAL4²²¹ (Lu et al., 2009) dorsal multidendritic neuron ddaF, with Scer\GAL4²²¹ (Lu et al., 2009)
Detailed Description
	Statement Reference Expression of Hsap\UBE3A[Scer\UAS.cRa] in a subset of DA sensory neurons using Scer\GAL4[221] causes decreased dendritic branching of ddaE and ddaF neurons. (Lu et al., 2009) Expression of Hsap\UBE3A[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF] at 25[o]C has no discernable effect on eye development. (Reiter et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference Hsap\UBE3A^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, faf^EP381 (Reiter et al., 2006) Hsap\UBE3A^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415 (Reiter et al., 2006) Hsap\UBE3A^Scer\UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, faf^EP381, pbl^EP3415 (Reiter et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Hsap\UBE3A[Scer\UAS.cRa] and pbl[EP3415] under the control of Scer\GAL4[GMR.PF] at 25[o]C rescues much of the effect of pbl[EP3415] overexpression. Although eyes still have a mild rough appearance, fewer than 3% exhibit necrotic foci and the ommatidia are much better organised and well formed than in pbl[EP3415] (Scer\GAL4[GMR.PF]) flies, including six to seven photoreceptor cells per ommatidium. The synergistic effect of pbl[EP3415] and faf[EP381] can be overcome by co-expression with Hsap\UBE3A[Scer\UAS.cRa] (all under the control of Scer\GAL4[GMR.PF]. (Reiter et al., 2006)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\UBE3A^Scer\UAS.cRa
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Ferdousy et al., 2011, Neurobiol. Disease 41(3): 669--677 Drosophila Ube3a regulates monoamine synthesis by increasing GTP cyclohydrolase I activity via a non-ubiquitin ligase mechanism. [FBrf0212886] Lu et al., 2009, Hum. Mol. Genet. 18(3): 454--462 The Drosophila homologue of the Angelman syndrome ubiquitin ligase regulates the formation of terminal dendritic branches. [FBrf0206993] Reiter et al., 2006, Hum. Mol. Genet. 15(18): 2825--2835 Expression of the Rho-GEF Pbl/ECT2 is regulated by the UBE3A E3 ubiquitin ligase. [FBrf0192865]

Allele Hsap\UBE3AScer\UAS.cRa

Allele Hsap\UBE3A^Scer\UAS.cRa