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General Information
Symbol
Dmel\AMPKαUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Mirouse
FlyBase ID
FBal0241422
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-AMPKα
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the AMPKα-RA isoform.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of AMPKαUAS.cMa under the control of Scer\GAL4GSG5966 (and RU486 feeding) leads to a significant decrease in lipid droplets (ie number, size and relative cellular content) in the adult midgut, as compared to controls.

The expression of AMPKαUAS.cMa under the control of Scer\GAL4hs.PB and induced by shift to 29[o]C for 2 days before the experiment induces lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut, as compared to controls.

Expression of one or two copies of SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi does not significantly reduce adult lifespan.

Ubiquitous expression of SNF1AScer\UAS.cMa (under the control of Scer\GAL4Ubi) does not affect levels of locomotion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of LamCUAS.cSa and AMPKαUAS.cMa under the control of Scer\GAL4fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The late pupal lethality observed in animals expressing AMPKαJF01951 under the control of Scer\GAL4tub.PU can be fully rescued by co-expression of AMPKαScer\UAS.cMa.

The pupal stage lethality observed in either AMPKα1 or AMPKα3 hemizygous males can be partially rescued by expression of AMPKαScer\UAS.cMa under the control of Scer\GAL4tub.PU.

Scer\GAL4Ubi-mediated expression of SNF1AScer\UAS.cMa rescues viability in SNF1A1 and SNF1A3 mutants.

Scer\GAL4109(2)80-mediated expression of SNF1AScer\UAS.cMa rescues the dendritic phenotype of SNF1A3 mutants.

Expression of SNF1AScer\UAS.cMa under the control of Scer\GAL4109(2)80 rescues the neuronal defects of SNF1A3 mutants.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
AMPKαScer\UAS.cMa
AMPKαUAS.cMa
SNF1AScer\UAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct of Mirouse
Secondary FlyBase IDs
    References (7)