FB2025_01 , released February 20, 2025
Allele: Dmel\Hsp60DRNAi.Sym.UAS
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General Information
Symbol
Dmel\Hsp60DRNAi.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0241521
Feature type
allele
Associated gene
Dmel\Hsp60D
Associated Insertion(s)
Carried in Construct
P{Sym-UAS-Hsp60D}
Key Links
Transgenic product class
RNAi_reagent
(Arya and Lakhotia, 2008)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Arya and Lakhotia, 2008)
Mutagen
in vitro construct
(Arya and Lakhotia, 2008)
Progenitor genotype
Carried in construct
P{Sym-UAS-Hsp60D}
Cytology
Description

Sense-antisense transcription of a 671 bp BglII-EcoRI fragment from the Hsp60D cDNA clone AT04835 (driven by two convergent arrays of UAS sequence) results in double stranded RNA (dsRNA) expression.

(Arya and Lakhotia, 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsp60D
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      NOT suppressed by
      Suppressor of
      Statement
      Reference

      Scer\GAL4GMR.PU, Hsp60DRNAi.Sym.UAS is a suppressor | partially of visible phenotype of Diap15/th[+], Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PU, Zzzz\CAG127Q.UAS.Tag:HA

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4elav.PU is a suppressor | partially of lethal | P-stage phenotype of Scer\GAL4elav.PU, Zzzz\CAG127Q.UAS.Tag:HA

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU is a suppressor | partially of abnormal phototaxis phenotype of Scer\GAL4GMR.PU, Zzzz\CAG127Q.UAS.Tag:HA

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU is a suppressor of visible phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU is a suppressor of abnormal phototaxis phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4elav.PU is a suppressor of lethal | P-stage phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4elav.PU

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor | partially of visible phenotype of rprGMR.PU

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of visible phenotype of Droncpro.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of lethal | pharate adult stage phenotype of Droncpro.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor of visible phenotype of aosGMR.PC

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor of visible phenotype of grimGMR.PH

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of visible phenotype of Scer\GAL4GMR.PF, egrUAS.cIa

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of visible phenotype of DroncΔN.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of lethal | pharate adult stage phenotype of DroncΔN.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor of visible phenotype of hidGMR.PU

      (Arya and Lakhotia, 2008)
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      NOT suppressed by
      Suppressor of
      Statement
      Reference

      Scer\GAL4GMR.PU, Hsp60DRNAi.Sym.UAS is a suppressor | partially of ommatidium phenotype of Diap15/th[+], Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU is a suppressor | partially of ommatidium phenotype of Scer\GAL4GMR.PU, Zzzz\CAG127Q.UAS.Tag:HA

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU is a suppressor of ommatidium phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU

      (Arya et al., 2010)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor | partially of eye phenotype of rprGMR.PU

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of eye phenotype of Droncpro.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of pigment cell phenotype of Droncpro.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of adult head phenotype of Droncpro.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor of eye phenotype of aosGMR.PC

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor of ommatidium phenotype of aosGMR.PC

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor of eye phenotype of grimGMR.PH

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of eye phenotype of Scer\GAL4GMR.PF, egrUAS.cIa

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of eye phenotype of DroncΔN.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PF is a suppressor of eye disc phenotype of DroncΔN.UAS, Scer\GAL4GMR.PF

      (Arya and Lakhotia, 2008)

      Hsp60DRNAi.Sym.UAS/Scer\GAL4GMR.PF is a suppressor of eye phenotype of hidGMR.PU

      (Arya and Lakhotia, 2008)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) suppresses the eye phenotypes caused by both weakly and strongly expressing Ncpro.Scer\UAS lines driven by Scer\GAL4GMR.PF, and all these animals eclose with normal head size.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) suppresses the eye phenotypes caused by expression of NcΔN.Scer\UAS under the control of Scer\GAL4GMR.PF, and all these animals eclose with normal head size.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) has no effect on the eye phenotypes caused by expression of thdsRNA.Scer\UAS.cLa under the control of Scer\GAL4GMR.PF.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) under the control of Scer\GAL4GMR.PF suppresses the eye phenotype caused by argosGMR.PC.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) suppresses the eye phenotype caused by expression of egrScer\UAS.cIa under the control of Scer\GAL4GMR.PF.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS suppresses the eye phenotypes caused by both weakly and strongly expressing Ncpro.Scer\UAS lines driven by Scer\GAL4GMR.PF, and all these animals eclose with normal head size.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS suppresses the eye phenotypes caused by expression of NcΔN.Scer\UAS under the control of Scer\GAL4GMR.PF, and all these animals eclose with normal head size.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS has no effect on the eye phenotypes caused by expression of thdsRNA.Scer\UAS.cLa under the control of Scer\GAL4GMR.PF.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS under the control of Scer\GAL4GMR.PF suppresses the eye phenotype caused by aosGMR.PC.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS suppresses the eye phenotype caused by expression of egrScer\UAS.cIa under the control of Scer\GAL4GMR.PF.

      (Arya and Lakhotia, 2008)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) with Scer\GAL4GMR.PU-driven Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 suppresses the eye degeneration phenotype resulting from Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression. The eyes show normal pigmentation and more or less regularly organised ommatidial arrays nearly comparable to those in wild-type eyes.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) via Scer\GAL4GMR.PU significantly suppresses the eye degeneration phenotype resulting from Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) with Scer\GAL4GMR.PU-driven Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 restores phototaxis.

      Co-expression of two copies of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) with Scer\GAL4GMR.PU-driven Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 partially restores phototaxis.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) via Scer\GAL4elav.PU partially suppresses the lethality resulting from Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression. The escapers are short lived with a maximum life span of six days.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) via Scer\GAL4elav.PU partially suppresses the lethality resulting from Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression. The lethal phase is shifted from early to late pupa, although no flies emerge.

      Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the partial suppression of the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression by P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS).

      Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the partial suppression of the eye degeneration phenotype caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression by P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS).

      The suppression of the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU by P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) is not much affected by heterozygosity for th5.

      The enhancement of the eye degeneration resulting from the overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU in a th5/+ background is not suppressed when P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) is co-expressed.

      Co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) via Scer\GAL4GMR.PU fails to suppress the eye degeneration in Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 and thdsRNA.Scer\UAS expressing flies.

      The pharate stage lethality due to co-expression of thdsRNA.Scer\UAS and Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU is not suppressed by simultaneous expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS).

      The eye phenotype resulting from simultaneous expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 and P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) via Scer\GAL4GMR.PU does not appear to be affected by co-expression of Uba2C175S.Scer\UAS.

      The suppression of the eye phenotype resulting from the expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4GMR.PU by co-expression of P{Sym-UAS-Hsp60D} (which carries Hsp60DSym.Scer\UAS and Hsp60Da.Sym.Scer\UAS) is less pronounced in the presence of Uba2C175S.Scer\UAS.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS with Scer\GAL4GMR.PU-driven Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 suppresses the eye degeneration phenotype resulting from Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression. The eyes show normal pigmentation and more or less regularly organised ommatidial arrays nearly comparable to those in wild-type eyes.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4GMR.PU significantly suppresses the eye degeneration phenotype resulting from Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS with Scer\GAL4GMR.PU-driven Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 restores phototaxis.

      Co-expression of two copies of Hsp60DdsRNA.Sym.Scer\UAS with Scer\GAL4GMR.PU-driven Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 partially restores phototaxis.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4elav.PU partially suppresses the lethality resulting from Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression. The escapers are short lived with a maximum life span of six days.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4elav.PU partially suppresses the lethality resulting from Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression. The lethal phase is shifted from early to late pupa, although no flies emerge.

      Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the Hsp60DdsRNA.Sym.Scer\UAS-mediated partial suppression of the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression.

      Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the Hsp60DdsRNA.Sym.Scer\UAS-mediated partial suppression of the eye degeneration phenotype caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression.

      The Hsp60DdsRNA.Sym.Scer\UAS-mediated suppression of the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU is not much affected by heterozygosity for th5.

      The enhancement of the eye degeneration resulting from the overexpression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU in a th5/+ background is not suppressed when Hsp60DdsRNA.Sym.Scer\UAS is co-expressed.

      Co-expression of Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4GMR.PU fails to suppress the eye degeneration in Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 and thdsRNA.Scer\UAS expressing flies.

      The pharate stage lethality due to co-expression of thdsRNA.Scer\UAS and Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU is not suppressed by simultaneous expression of Hsp60DdsRNA.Sym.Scer\UAS.

      The eye phenotype resulting from simultaneous expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 and Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4GMR.PU does not appear to be affected by co-expression of Uba2C175S.Scer\UAS.

      The Hsp60DdsRNA.Sym.Scer\UAS-mediated suppression of the eye phenotype resulting from the expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4GMR.PU is less pronounced in the presence of Uba2C175S.Scer\UAS.

      (Arya et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Associated with: Hsp60Da.Sym.Scer\UAS.

      (Arya and Lakhotia, 2008)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Hsp60DRNAi.Sym.UAS
      Hsp60DSym.Scer\UAS
      Hsp60DdsRNA.Sym.Scer\UAS
      Hsp60DdsRNA.Sym.UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0241522
      References (3)