Allele Dmel\csw^{N308D.Scer\UAS.P\T}

General Information
Symbol	Dmel\csw^{N308D.Scer\UAS.P\T}	Species	D. melanogaster
Name		FlyBase ID	FBal0241536
Feature type	allele	Associated gene	Dmel\csw

Allele class
Mutagen	in vitro construct - amino acid replacement, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Oishi et al., 2006) in vitro construct - regulatory fusion (Oishi et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of csw containing the amino acid replacement N287D (this mutation is equivalent to the N308D mutation in the human ortholog Hsap\PTPN11, which is a mutation associated with Noonan syndrome in humans). (Oishi et al., 2006)
Carried in construct	P{UASp-csw.N308D} (Oishi et al., 2009, Oishi et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^tub.PU (Oishi et al., 2006)
Phenotype Manifest In
	wing vein \| ectopic, with Scer\GAL4^tub.PU (Oishi et al., 2006)
Detailed Description
	Statement Reference Expression of one copy of csw[N308D.Scer\UAS.P\T] under the control of Scer\GAL4[tub.PU] does not result in lethality, whereas expression of two copies results in lethality. Flies expressing one copy of csw[N308D.Scer\UAS.P\T] under the control of Scer\GAL4[tub.PU] have ectopic wing veins, mainly in the peripheral areas of vein L2 and the posterior cross vein. (Oishi et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by aos[+]/aos^Δ7 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by bs[+]/bs^k07909 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by Dl^6B/Dl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by Dl^9P/Dl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by E(spl)m8-HLH^rv1/E(spl)m8-HLH[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by Gap1[+]/RasGAP1^B2 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by hop²⁷/hop[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by lace[+]/lace² (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by lack[+]/lack^KG07014 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, enhanceable by sty[+]/sty²²⁶ (Oishi et al., 2006)
NOT Enhanced by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-enhanceable by Cbl^KG03080/Cbl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-enhanceable by dos[+]/dos^P115 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-enhanceable by phl⁷/phl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-enhanceable by spi¹/spi[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-enhanceable by spi²⁵⁹/spi[+] (Oishi et al., 2006)
Suppressed by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by dpp¹⁰⁶³⁸/dpp[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by dpp^d6/dpp[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by dpp^d-ho/dpp[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by drk[+]/drk^e0A (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Dsor1[+]/Dsor1^LH110 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Dsor1[+]/Dsor1^S-1221 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by E(spl)m8-HLH¹/E(spl)m8-HLH[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Egfr[+]/Egfr^t1 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by hop^Tum/hop[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Hsp83[+]/Hsp83^e6A (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Hsp83^e6D/Hsp83[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Mad[+]/Mad^1-2 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Mad^8-2/Mad[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by pnt[+]/pnt¹²³⁰ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by pnt[+]/pnt^Δ88 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Ras85D^N17.Scer\UAS, Scer\GAL4^tub.PU (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by rho^7M43/rho⁺ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by rho^ve-1/rho⁺ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by rl⁺/rl^10a (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by S[+]/S^IIN (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by S^k09530/S[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Sos[+]/Sos^34Ea-6 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by Su(H)[+]/Su(H)¹ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by tkv¹/tkv[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by tkv⁷/tkv[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by vn[+]/vn¹⁰⁵⁶⁷ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, suppressible by vn[+]/vn^C221 (Oishi et al., 2006)
NOT suppressed by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-suppressible by Cbl^KG03080/Cbl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-suppressible by dos[+]/dos^P115 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-suppressible by phl⁷/phl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-suppressible by spi¹/spi[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has visible phenotype, non-suppressible by spi²⁵⁹/spi[+] (Oishi et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by aos[+]/aos^Δ7 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by bs[+]/bs^k07909 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by Dl^6B/Dl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by Dl^9P/Dl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by E(spl)m8-HLH^rv1/E(spl)m8-HLH[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by Gap1[+]/RasGAP1^B2 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by hop²⁷/hop[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by lace[+]/lace² (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by lack[+]/lack^KG07014 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by Stat92E[+]/Stat92E⁰⁶³⁴⁶ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, enhanceable by sty[+]/sty²²⁶ (Oishi et al., 2006)
NOT Enhanced by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-enhanceable by Cbl^KG03080/Cbl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-enhanceable by dos[+]/dos^P115 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-enhanceable by phl⁷/phl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-enhanceable by spi¹/spi[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-enhanceable by spi²⁵⁹/spi[+] (Oishi et al., 2006)
Suppressed by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by dpp¹⁰⁶³⁸/dpp[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by dpp^d6/dpp[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by dpp^d-ho/dpp[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by drk[+]/drk^e0A (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Dsor1[+]/Dsor1^LH110 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Dsor1[+]/Dsor1^S-1221 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by E(spl)m8-HLH¹/E(spl)m8-HLH[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Egfr[+]/Egfr^t1 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by hop^Tum/hop[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Hsp83[+]/Hsp83^e6A (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Hsp83^e6D/Hsp83[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Mad[+]/Mad^1-2 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Mad^8-2/Mad[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by pnt[+]/pnt¹²³⁰ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by pnt[+]/pnt^Δ88 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Ras85D^N17.Scer\UAS, Scer\GAL4^tub.PU (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by rho^7M43/rho⁺ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by rho^ve-1/rho⁺ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by rl⁺/rl^10a (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by S[+]/S^IIN (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by S^k09530/S[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Sos[+]/Sos^34Ea-6 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by Su(H)[+]/Su(H)¹ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by tkv¹/tkv[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by tkv⁷/tkv[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by vn[+]/vn¹⁰⁵⁶⁷ (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, suppressible by vn[+]/vn^C221 (Oishi et al., 2006)
NOT suppressed by
Statement Reference Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-suppressible by Cbl^KG03080/Cbl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-suppressible by dos[+]/dos^P115 (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-suppressible by phl⁷/phl[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-suppressible by spi¹/spi[+] (Oishi et al., 2006) Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} has wing vein \| ectopic phenotype, non-suppressible by spi²⁵⁹/spi[+] (Oishi et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	csw^{N308D.Scer\UAS.P\T}/Scer\GAL4^GMR.PU partially rescues csw^lf (Oishi et al., 2006)
Comments	Expression of csw[N308D.Scer\UAS.P\T] under the control of Scer\GAL4[GMR.PU] partially rescues the rough eye phenotype of csw[lf] flies. (Oishi et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	csw^{N308D.Scer\UAS.P\T} csw^{N308D.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Oishi et al., 2009, Hum. Mol. Genet. 18(1): 193--201 Phosphatase-defective LEOPARD syndrome mutations in PTPN11 gene have gain-of-function effects during Drosophila development. [FBrf0207302] Oishi et al., 2006, Hum. Mol. Genet. 15(4): 543--553 Transgenic Drosophila models of Noonan syndrome causing PTPN11 gain-of-function mutations. [FBrf0190828]

Allele Dmel\cswN308D.Scer\UAS.P\T

Allele Dmel\csw^{N308D.Scer\UAS.P\T}