Allele Dmel\csw^{Y279C.Scer\UAS.P\T}

General Information
Symbol	Dmel\csw^{Y279C.Scer\UAS.P\T}	Species	D. melanogaster
Name		FlyBase ID	FBal0241537
Feature type	allele	Associated gene	Dmel\csw

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Oishi et al., 2009) in vitro construct - regulatory fusion (Oishi et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of csw containing the amino acid replacement Y258C (this mutation is equivalent to the Y279C mutation in the human ortholog Hsap\PTPN11, which is a mutation associated with LEOPARD syndrome in humans). (Oishi et al., 2009)
Carried in construct	P{UASp-csw.Y279C} (Oishi et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	crossvein, with Scer\GAL4^tub.PU (Oishi et al., 2009) eye, with Scer\GAL4^tub.PU (Oishi et al., 2009) ommatidium, with Scer\GAL4^tub.PU (Oishi et al., 2009) photoreceptor cell R7 \| ectopic, with Scer\GAL4^tub.PU (Oishi et al., 2009) wing, with Scer\GAL4^tub.PU (Oishi et al., 2009) wing vein, with Scer\GAL4^tub.PU (Oishi et al., 2009) wing vein L2, with Scer\GAL4^tub.PU (Oishi et al., 2009)
Detailed Description
	Statement Reference Expression of csw[Y279C.Scer\UAS] under the control of Scer\GAL4[tub] does not affect viability or fertility. While expression does not appear to affect early development, all Scer\GAL4[tub]-csw[Y279C.Scer\UAS] adult flies display ectopic wing veins, mainly in the peripheral area of L2 and the posterior cross vein. Additionally, there are numerous small ectopic vein structures widely spread over the entire csw[Y279C.Scer\UAS]-expressing area on the wing. Flies expressing csw[Y279C.Scer\UAS] under the control of Scer\GAL4[tub] exhibit a rough eye phenotype. although the size of the eye is not obviously affected, the arrangement of the ommatidia is altered. The ommatidia are not symmetrically organised, with microscopy sections revealing the presence of multiple R7 photoreceptors in 24.5% of ommatidia. These eyes also display abnormalities of ommatidial rotation (20% of ommatidia). Flies expressing both csw[Y279C.Scer\UAS] and csw[R465M.Scer\UAS] under the control of Scer\GAL4[tub] results in strong suppression of ectopic wing vein formation. The number of R7 photoreceptors is normal, along with ommatidial rotation. (Oishi et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has eye phenotype, enhanceable by sty²²⁶/Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has ommatidium phenotype, enhanceable by sty²²⁶/Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has photoreceptor cell R7 \| ectopic phenotype, enhanceable by sty²²⁶/Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, enhanceable by aos^Δ7/Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, enhanceable by RasGAP1^B2, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, enhanceable by sty²²⁶/Scer\GAL4^tub.PU (Oishi et al., 2009)
NOT Enhanced by
Statement Reference Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, non-enhanceable by Scer\GAL4^tub.PU/drk^e0A (Oishi et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has eye phenotype, suppressible by Egfr^t1, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has eye phenotype, suppressible by S^IIN, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has ommatidium phenotype, suppressible by Egfr^t1, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has ommatidium phenotype, suppressible by S^IIN, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has photoreceptor cell R7 \| ectopic phenotype, suppressible by Egfr^t1, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has photoreceptor cell R7 \| ectopic phenotype, suppressible by S^IIN, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, suppressible \| partially by spi¹/Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, suppressible \| partially by vn^C221/Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, suppressible by Egfr^t1, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, suppressible by Ras85D^e1B, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, suppressible by rl^10a/Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, suppressible by S^IIN, Scer\GAL4^tub.PU (Oishi et al., 2009) Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, suppressible by Scer\GAL4^tub.PU/Sos^34Ea-6 (Oishi et al., 2009)
NOT suppressed by
Statement Reference Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} has wing vein phenotype, non-suppressible by Scer\GAL4^tub.PU/drk^e0A (Oishi et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference The presence of a sty[226] background significantly enhances ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. A sty[226] background enhances the csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) rough eye phenotype, with the number of R7 in each ommatidium increasing. The presence of a Egfr[t1] background significantly suppresses ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. A Egfr[t1] background suppresses the csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) rough eye phenotype, normalizing the numbers of R7 and ommatidial rotation. The presence of a drk[e0A] background does not affect ectopic wing vein formation in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. The presence of a spi[1] background causes minor suppression of ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. The presence of a vn[C221] background causes minor suppression of ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. The presence of a argos[Δ7] background significantly enhances ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. The presence of a S[IIN] background significantly suppresses ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. A S[IIN] background suppresses the csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) rough eye phenotype, normalizing the numbers of R7 and ommatidial rotation. The presence of a Sos[34Ea-6] background significantly suppresses ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. The presence of a Ras85D[e1B] background significantly suppresses ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. A Ras85D[e1B] background suppresses the csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) rough eye phenotype, normalizing the numbers of R7 and ommatidial rotation. The presence of a Gap1[B2] background significantly enhances ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. The presence of a rl[10a] background significantly suppresses ectopic wing vein formation found in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. (Oishi et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	csw^{Y279C.Scer\UAS.P\T} is rescued by csw^{R465M.Scer\UAS.P\T}/Scer\GAL4^tub.PU (Oishi et al., 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	csw^{Y279C.Scer\UAS.P\T} csw^{Y279C.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Oishi et al., 2009, Hum. Mol. Genet. 18(1): 193--201 Phosphatase-defective LEOPARD syndrome mutations in PTPN11 gene have gain-of-function effects during Drosophila development. [FBrf0207302]

Allele Dmel\cswY279C.Scer\UAS.P\T

Allele Dmel\csw^{Y279C.Scer\UAS.P\T}