Allele Dmel\Cerk¹

General Information
Symbol	Dmel\Cerk¹	Species	D. melanogaster
Name		FlyBase ID	FBal0241656
Feature type	allele	Associated gene	Dmel\Cerk
Also Known As	dcerk¹

Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Dasgupta et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{GT1}Cerk^BG01100 (Dasgupta et al., 2009, Dasgupta et al., 2009)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion, leaving approximately 6kb of the original insertion in place. This results in a significant reduction in transcript levels compared to wild type. (Dasgupta et al., 2009)
Caused by insertion	P{?GT1}Cerk¹ (Dasgupta et al., 2009, Dasgupta et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective (Dasgupta et al., 2009) partially lethal - majority die (Dasgupta et al., 2009) viable (Yuan et al., 2011)
Phenotype Manifest In
	eye photoreceptor cell (Dasgupta et al., 2009, Dasgupta et al., 2009) rhabdomere (Dasgupta et al., 2009, Dasgupta et al., 2009)
Detailed Description
	Statement Reference Homozygous Cerk[1] mutants are viable and have decreased fertility. (Yuan et al., 2011) Mutants show fertility defects. 5-day old mutant adults show severe photoreceptor degeneration; the cells appear vacuolated with almost no intact rhabdomeres. This degeneration phenotype is light-dependent as it is not seen in flies raised in the dark. Mutant flies raised in the dark do not respond to light stimulus when assayed by electroretinogram recordings. (Dasgupta et al., 2009) Mutant adults show progressive light induced degeneration of the rhabdomeres; rhabdomeres are seen in 1 day-old flies, but 5- and 17-day old flies have hardly any rhabdomeres and show extensive vacuolation of the photoreceptors. (Dasgupta et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Cerk¹ has neurophysiology defective phenotype, suppressible by CDase^Scer\UAS.cAa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Dasgupta et al., 2009) Cerk¹ has viable phenotype, suppressible by bwa^unspecified/bwa[+] (Yuan et al., 2011)
NOT suppressed by
Statement Reference Cerk¹ has neurophysiology defective phenotype, non-suppressible by Prosβ6¹ (Dasgupta et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference Cerk¹ has eye photoreceptor cell phenotype, suppressible by CDase^Scer\UAS.cAa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Dasgupta et al., 2009) Cerk¹ has rhabdomere phenotype, suppressible by CDase^Scer\UAS.cAa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Dasgupta et al., 2009)
NOT suppressed by
Statement Reference Cerk¹ has eye photoreceptor cell phenotype, non-suppressible by Prosβ6¹ (Dasgupta et al., 2009) Cerk¹ has rhabdomere phenotype, non-suppressible by Prosβ6¹ (Dasgupta et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Introduction of bwa[unspecified] into a Cerk[1] mutant background results in lethality. (Yuan et al., 2011) Expression of CDase[Scer\UAS.cAa] under the control of Scer\GAL4[GMR.PF] rescues the lack of a light-induced electroretinogram response seen in Cerk[1] flies. The light-induced retinal degeneration that is seen in Cerk[1] flies is also rescued by expression of CDase[Scer\UAS.cAa] under the control of Scer\GAL4[GMR.PF]. Pros26[1] cannot rescue the lack of a light-induced electroretinogram response and the retinal degeneration that is seen in Cerk[1] flies. (Dasgupta et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Cerk¹ is rescued by Scer\GAL4^Act.PU/Cerk^Scer\UAS.cDa (Dasgupta et al., 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Cerk¹ dcerk¹ (Dasgupta et al., 2009, Dasgupta et al., 2009, Yuan et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Yuan et al., 2011, Mol. Biol. Cell 22(1): 33--43 CDase is a pan-ceramidase in Drosophila. [FBrf0212735] Dasgupta et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(47): 20063--20068 Ceramide kinase regulates phospholipase C and phosphatidylinositol 4, 5, bisphosphate in phototransduction. [FBrf0209371]
Supplementary material	Dasgupta et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(47): Supporting information. [FBrf0210341]

Allele Dmel\Cerk1

Allele Dmel\Cerk¹