UAS regulatory sequences drive expression of an inverted repeat.
Adult females expressing Cul2HM05237 RNAi under the control of the Scer\GAL4bab1-Gal4 or the Scer\GAL4tj.PU driver have no ovaries.
Expression of Cul2HM05237 RNAi under control of Scer\GAL4ptc.PU together with temperature manipulations to limit the strength of RNAi expression during development or after eclosion shows that Cul2 contributes to germaria formation during development as well as in adulthood: The majority of females kept at high temperature during development (to maximize the Cul2HM05237 RNAi expression) and low temperature after eclosion (to minimize the Cul2HM05237 RNAi expression in adults) lack germaria altogether. Conversely, females kept at low temperatures during development and shifted to high temperatures after eclosion also display defects in germarium development and a higher proportion of germaria that show highly increased number of spectrosome-containing cells compared to controls.
Expression of Cul2HM05237 in somatic support cells under the control of any of Scer\GAL4tj-NP1624, Scer\GAL4A3.eya or Scer\GAL4c784 (limited to a week from eclosion onwards using Scer\GAL80ts.αTub84B) leads to strong defects in germline enclosure in 100% of testes.