Adult females expressing Cul2^HM05237 RNAi under the control of the Scer\GAL4^bab1-Gal4 or the Scer\GAL4^tj.PU driver have no ovaries.

Expression of Cul2^HM05237 RNAi under control of Scer\GAL4^ptc.PU together with temperature manipulations to limit the strength of RNAi expression during development or after eclosion shows that Cul2 contributes to germaria formation during development as well as in adulthood: The majority of females kept at high temperature during development (to maximize the Cul2^HM05237 RNAi expression) and low temperature after eclosion (to minimize the Cul2^HM05237 RNAi expression in adults) lack germaria altogether. Conversely, females kept at low temperatures during development and shifted to high temperatures after eclosion also display defects in germarium development and a higher proportion of germaria that show highly increased number of spectrosome-containing cells compared to controls.

Expression of Cul2^HM05237 in somatic support cells under the control of any of Scer\GAL4^tj-NP1624, Scer\GAL4^A3.eya or Scer\GAL4^c784 (limited to a week from eclosion onwards using Scer\GAL80^ts.αTub84B) leads to strong defects in germline enclosure in 100% of testes.