FB2025_01 , released February 20, 2025
Allele: Dmel\Cul2HM05237
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General Information
Symbol
Dmel\Cul2HM05237
Species
D. melanogaster
Name
FlyBase ID
FBal0241728
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
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Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
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Modifiers Based on Experimental Evidence ( 0 )
Disease
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Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adult females expressing Cul2HM05237 RNAi under the control of the Scer\GAL4bab1-Gal4 or the Scer\GAL4tj.PU driver have no ovaries.

Expression of Cul2HM05237 RNAi under control of Scer\GAL4ptc.PU together with temperature manipulations to limit the strength of RNAi expression during development or after eclosion shows that Cul2 contributes to germaria formation during development as well as in adulthood: The majority of females kept at high temperature during development (to maximize the Cul2HM05237 RNAi expression) and low temperature after eclosion (to minimize the Cul2HM05237 RNAi expression in adults) lack germaria altogether. Conversely, females kept at low temperatures during development and shifted to high temperatures after eclosion also display defects in germarium development and a higher proportion of germaria that show highly increased number of spectrosome-containing cells compared to controls.

Expression of Cul2HM05237 in somatic support cells under the control of any of Scer\GAL4tj-NP1624, Scer\GAL4A3.eya or Scer\GAL4c784 (limited to a week from eclosion onwards using Scer\GAL80ts.αTub84B) leads to strong defects in germline enclosure in 100% of testes.

External Data
Interactions
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Phenotypic Class
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Xenogenetic Interactions
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Complementation and Rescue Data
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Mutant
Wild-type
Stocks (1)
Notes on Origin
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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Cul-2HM05237
Cul2HM05237
Name Synonyms
Secondary FlyBase IDs
    References (4)