FB2025_01 , released February 20, 2025
Allele: Dmel\CDase1
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General Information
Symbol
Dmel\CDase1
Species
D. melanogaster
Name
FlyBase ID
FBal0241853
Feature type
allele
Associated gene
Dmel\CDase
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Yuan et al., 2011)
Mutagen
ethyl methanesulfonate
(Acharya et al., 2008)
Progenitor genotype
Cytology
Description

Amino acid replacement: W641term.

(Acharya et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:30,465,308..30,465,308 [+]
Nucleotide change:

G30465308A

Reported nucleotide change:

G?A

Amino acid change:

W641term | CDase-PA; W641term | CDase-PB; W641term | CDase-PC; W641term | CDase-PD; W641term | CDase-PE; W641term | CDase-PF

Reported amino acid change:

W641term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase base on reported amino acid change.

(Acharya et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CDase1 is homozygous and hemizygous lethal.

      (Yuan et al., 2011)

      Animals in which almost the entire eye is homozygous (generated using the eyFLP system) have a normal rhabdomere architecture at 15 days after eclosion.

      Hemizygous CDase1 adult escapers (which survive due to the presence of a closely linked suppressor mutation on the same chromosome that suppresses some of the lethality caused by CDase1) show severe photoreceptor degeneration 5 days after eclosion when they are raised in regular light and dark cycles. Many photoreceptor cells are missing their rhabdomeres and have vacuolated cell bodies. This photoreceptor degeneration is not suppressed by feeding with sphingosine. 5 day old mutant flies raised in constant darkness do not show photoreceptor degeneration, however they do not show an electroretinogram response (even to high-intensity light).

      Mutant third instar larvae show increased apoptosis in eye imaginal discs, mostly anterior to the morphogenetic furrow, compared to control larvae. The imaginal discs of irradiated mutant third instar larvae show higher levels of apoptosis than those of irradiated control larvae.

      (Acharya et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      CDase1 has lethal | recessive phenotype, suppressible by Scer\GAL4Act.PU/bwaUAS.cYa

      (Yuan et al., 2011)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      CDase1 has eye photoreceptor cell phenotype, suppressible by BacA\p35GMR.PH

      (Acharya et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Overexpression of bwaScer\UAS.cYa driven by Scer\GAL4Act.PU suppresses the lethality of CDase1.

      (Yuan et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of BacA\p35GMR.PH suppresses the light-induced retinal degeneration that is seen in CDase1 flies.

      (Acharya et al., 2008)
      Complementation and Rescue Data
      Rescued by

      CDase1 is rescued by CDase+tAa

      (Acharya et al., 2008)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CDase1
      cdase1
      (Yuan et al., 2011, Acharya et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)