Allele Dmel\tutl^{AT02763.Scer\UAS}

General Information
Symbol	Dmel\tutl^{AT02763.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0241904
Feature type	allele	Associated gene	Dmel\tutl

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Al-Anzi and Wyman, 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of tutl coding sequences corresponding to the AT02763 isoform. (Al-Anzi and Wyman, 2009)
Carried in construct	P{UAS-tutl.AT02763} (Al-Anzi and Wyman, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^elav-C155 (Al-Anzi and Wyman, 2009) neuroanatomy defective, with Scer\GAL4^G14 (Al-Anzi and Wyman, 2009) neuroanatomy defective, with Scer\GAL4^GMR.PF (Al-Anzi and Wyman, 2009) neuroanatomy defective, with Scer\GAL4^how-24B (Al-Anzi and Wyman, 2009) neuroanatomy defective, with Scer\GAL4^sca-109-68 (Al-Anzi and Wyman, 2009) neuroanatomy defective, with Scer\GAL4^sim.PS (Al-Anzi and Wyman, 2009)
Phenotype Manifest In
	commissure, with Scer\GAL4^elav-C155 (Al-Anzi and Wyman, 2009) commissure, with Scer\GAL4^sim.PS (Al-Anzi and Wyman, 2009) intersegmental nerve branch ISNd of A1-7, with Scer\GAL4^elav-C155 (Al-Anzi and Wyman, 2009) intersegmental nerve branch ISNd of A1-7, with Scer\GAL4^G14 (Al-Anzi and Wyman, 2009) intersegmental nerve branch ISNd of A1-7, with Scer\GAL4^how-24B (Al-Anzi and Wyman, 2009) intersegmental nerve branch ISNd of A1-7, with Scer\GAL4^sca-109-68 (Al-Anzi and Wyman, 2009) intersegmental nerve branch ISNd of A1-7, with Scer\GAL4^sim.PS (Al-Anzi and Wyman, 2009) longitudinal connective, with Scer\GAL4^elav-C155 (Al-Anzi and Wyman, 2009) longitudinal connective, with Scer\GAL4^sim.PS (Al-Anzi and Wyman, 2009) neuropil, with Scer\GAL4^elav-C155 (Al-Anzi and Wyman, 2009) optic lobe, with Scer\GAL4^GMR.PF (Al-Anzi and Wyman, 2009) transverse nerve, with Scer\GAL4^elav-C155 (Al-Anzi and Wyman, 2009) transverse nerve, with Scer\GAL4^G14 (Al-Anzi and Wyman, 2009) transverse nerve, with Scer\GAL4^how-24B (Al-Anzi and Wyman, 2009) transverse nerve, with Scer\GAL4^sca-109-68 (Al-Anzi and Wyman, 2009) transverse nerve, with Scer\GAL4^sim.PS (Al-Anzi and Wyman, 2009)
Detailed Description
	Statement Reference Pan-neuronal expression of the diffusible tutl[GH15753.Scer\UAS] isoform under the control of Scer\GAL4[elav-C155] produces Fas2-positive tracks that crisscross the midline. The same phenotype is produced upon expression in the midline, driven by Scer\GAL4[sim.PS]. Overexpression of tutl[AT02763.Scer\UAS] under the control of Scer\GAL4[sca-109-68], Scer\GAL4[how-24B] or Scer\GAL4[G14] produces excessive branching of the ISNd nerve, stalling of motor nerves, with some nerves, such as the transverse nerve, innervating muscles that are not their normal targets. Overexpression of tutl[AT02763.Scer\UAS] in a wild-type background using the retina-specific Scer\GAL4[GMR.PF] produces neuronal defects in that many retinal axons stall before reaching their targets and some sprout extra axonal processes that can invade the cortex. Pan-neuronal overexpression of tutl[AT02763.Scer\UAS] under the control of Scer\GAL4[elav-C155] promotes neuronal invasiveness, with some neuronal cell bodies invading the normally cell-free neuropil layer. (Al-Anzi and Wyman, 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^elav-C155/tutl^{AT02763.Scer\UAS} rescues Df(2L)ed-dp/tutl^ex383 (Al-Anzi and Wyman, 2009) Scer\GAL4^elav-C155/tutl^{AT02763.Scer\UAS} rescues tutl^ex383 (Al-Anzi and Wyman, 2009) tutl^{AT02763.Scer\UAS}/Scer\GAL4^sim.PS rescues Df(2L)ed-dp/tutl^ex383 (Al-Anzi and Wyman, 2009) tutl^{AT02763.Scer\UAS}/Scer\GAL4^sim.PS rescues tutl^ex383 (Al-Anzi and Wyman, 2009)
Fails to rescue	tutl^{AT02763.Scer\UAS}/Scer\GAL4^repo fails to rescue Df(2L)ed-dp/tutl^ex383 (Al-Anzi and Wyman, 2009) tutl^{AT02763.Scer\UAS}/Scer\GAL4^repo fails to rescue tutl^ex383 (Al-Anzi and Wyman, 2009)
Comments	Post-mitotic neuronal expression of tutl[AT02763.Scer\UAS] under the control of either Scer\GAL4[sim.PS] or Scer\GAL4[elav-C155] fully rescues the midline crossing defects found in tutl[ex383] and tutl[ex383]/Df(2L)ed-dp mutants. Expression under the control of Scer\GAL4[repo] does no rescue the phenotype, indicating that expression near the midline is important for rescue. Post-mitotic neuronal expression of tutl[AT02763.Scer\UAS] under the control of Scer\GAL4[elav-C155] is sufficient to rescue the adult lethality found in tutl[ex383] mutants. Expression of tutl[AT02763.Scer\UAS] via Scer\GAL4[elav-C155] can reduce the motor axon projection defects found in tutl[ex383] homozygotes. Expression of either tutl[AT02763.Scer\UAS] driven by the pan-neuronal driver Scer\GAL4[elav-C155] rescues most aspects of tutl[k14703]/tutl[ex383] mutant eye defects. (Al-Anzi and Wyman, 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	tutl^{AT02763.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Al-Anzi and Wyman, 2009, Neural Dev. 4: 31 The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding. [FBrf0209104]

Allele Dmel\tutlAT02763.Scer\UAS

Allele Dmel\tutl^{AT02763.Scer\UAS}