Amino acid replacement: D292N.
UASt regulatory sequences drive expression of Syt4 coding sequence where the C2A Ca[2+] binding domain is mutated through an amino acid residue change (D292N).
neuromuscular junction, with Scer\GAL4Mhc.PW
NMJ bouton, with Scer\GAL4Mhc.PW
Expression of Syt4D292N.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW fails to promote synaptic growth as efficiently as expression of a wild-type construct, although some synaptic growth is still observed.