Amino acid replacement: D427N.
UASt regulatory sequences drive expression of Syt4 coding sequence where the C2B Ca[2+] binding domain is mutated through two amino acid residue changes (D427N and D429N).
Amino acid replacement: D429N.
neuromuscular junction, with Scer\GAL4Mhc.PW
NMJ bouton, with Scer\GAL4Mhc.PW
Expression of Syt4D427N.D429N.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW fails to promote synaptic growth.
Overexpression of Syt4D427N.D429N.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW eliminates the ability of Syt4 to induce presynaptic release.