Amino acid replacement: S284D.
UASt regulatory sequences drive expression of Syt4 coding sequence where the C2A Ca[2+] binding domain is mutated through an amino acid residue change (S284D), although this change maintains the phosphorylation site.
neuromuscular junction, with Scer\GAL4Mhc.PW
NMJ bouton, with Scer\GAL4Mhc.PW
Expression of Syt4S284D.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW promotes synaptic growth similar to wild-type.
Overexpression of Syt4S284A.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW promotes the ability of Syt4 to induce presynaptic release.