FlyBase Allele Report: Dmel\orb2[ΔQ]

General Information

Symbol

Dmel\orb2^ΔQ

Species

D. melanogaster

Name

FlyBase ID

FBal0241952

Feature type

allele

Associated gene

Dmel\orb2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Keleman et al., 2007)

gene targeting by homologous recombination

(Keleman et al., 2007)

Progenitor genotype

Cytology

Description

In-frame deletion of 54 amino acids that specifically removes the glutamine rich region from both orb2 isoforms.

(Keleman et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior

(Keleman et al., 2007)

abnormal courtship behavior (with orb2^Δ)

(Keleman et al., 2007)

abnormal memory

(Keleman et al., 2007)

abnormal memory (with orb2^Δ)

(Keleman et al., 2007)

male fertile (with Df(3L)ED4416)

(Xu et al., 2012)

viable

(Keleman et al., 2007)

viable (with orb2^Δ)

(Keleman et al., 2007)

Phenotype Manifest In

axoneme

(Xu et al., 2012)

spermatid cyst

(Shao et al., 2023, Xu et al., 2014)

sperm individualization complex

(Xu et al., 2014, Xu et al., 2012)

Detailed Description

Statement

Reference

Testes of mutant males sometimes contain misoriented spermatid cysts where the clustered spermatid nuclei are found apically (rather than basally). Individualisation complexes (ICs) in spermatid cysts are sometimes located in the spermatocyte region of the testis (instead of towards the basal end of the testis as in wild type) and ICs moving in opposite directions are sometimes seen.

(Xu et al., 2014)

No meiosis defects are seen in mutant males. Flagellar axoneme bundles are over-elongated, extending into the spermatogonia region in 70% of mutant testes. In some cases, the elongating axoneme pushes against the testis wall and causes the muscle layer encasing the apical tip to rupture. The fraction of testes containing scattered individualisation complexes is increased in mutant testes compared to wild type (35% compared to approximately 6%) and there is a reduction in the number of individualisation complexes per testis.

(Xu et al., 2012)

orb2^ΔQ mutants do not exhibit any obvious defects in locomotion and activity. Flies return to a standing position following vortexing at a similar rate to wild type flies and show normal climbing ability.

(Hafer et al., 2011)

orb2^ΔQ homozygotes and orb2^ΔQ/orb2^Δ transheterozygotes do not exhibit any gross morphological defects, including in their overall brain and mushroom body anatomy.

Courtship behavior and short-term memory of courtship conditioning are both intact in orb2^ΔQ and orb2^ΔQ/orb2^Δ mutants.

Courtship suppression (where the male understands if certain females are receptive to mating or not) is completely abolished in both orb2^ΔQ and orb2^ΔQ/orb2^Δ mutants. orb2^ΔQ/orb2^Δ heterozygotes show an intermediate level of suppression, suggesting that orb2 levels may be a limiting factor in long-term memory formation.

Memory persists at its initial levels in orb2^ΔQ mutants for up to 6 hours after training, and is indistinguishable from memory levels in control males. However, although control males retained these initial memory levels for at least 24 hours, memory is completely lost in the mutants by 9 hours post-traning. This time course of memory decay in orb2^ΔQ mutants trained for 5 hours match that observed for wild-type males trained or only 1 hour.

(Keleman et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

aPKC^EX48, orb2^ΔQ/orb2[+] has spermatid cyst phenotype

(Xu et al., 2014)

aPKC^Exc55, orb2^ΔQ/orb2[+] has spermatid cyst phenotype

(Xu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

aPKC^Exc55/+ ; orb2^ΔQ/+ and aPKC^EX48/+ ; orb2^ΔQ/+ double heterozygous males sometimes contain misoriented spermatid cysts.

(Xu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^fru.P1.D/orb2^A.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^c747/orb2^A.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^Mef2.247/orb2^A.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^Tab2-201Y/orb2^A.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4¹⁴⁷¹/orb2^A.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^H24/orb2^A.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^fru.P1.D/orb2^B.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^c747/orb2^B.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is rescued by Scer\GAL4^Mef2.247/orb2^B.UAS

(Keleman et al., 2007)

Not rescued by

orb2^ΔQ/orb2^Δ is not rescued by orb2^A.UAS/Scer\GAL4^c739

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is not rescued by Scer\GAL4^c305a/orb2^A.UAS

(Keleman et al., 2007)

orb2^ΔQ/orb2^Δ is not rescued by Scer\GAL4^GH146/orb2^A.UAS

(Keleman et al., 2007)

Comments

Expression of orb2^A.Scer\UAS or orb2^B.Scer\UAS under the control of Scer\GAL4^fru-GAL4, Scer\GAL4^c747 or Scer\GAL4^Mef2.247 restores 24 hour memory in orb2^Δ/orb2^ΔQ transheterozygotes.

Using the TARGET system (using Scer\GAL80^{ts.alphaTub84B} to suppress Scer\GAL4 expression a certain points during development) to express either orb2^A.Scer\UAS or orb2^B.Scer\UAS (under the control of Scer\GAL4^Mef2.247) in the mushroom bodiesfully rescues long-term memory in orb2^Δ/orb2^ΔQ mutants when these flies are raised and tested at 29[o]C, but not when they are raised and tested at 18[o]C. Long-term memory is also restored when flies are raised at 18[o]C and then shifted to 29[o]C immediately after eclosion, thereby providing orb2 function only in the adults. The reciprocal experiment, in which flies are raised at 29[O]C, but shifted to 18[o]C as adults, does not restore long-term memory. Flies that are shifted up to 29[o]C just 2 hours before training show normal long-term memory, whereas those that are shifted immediately after training have no long-term memory. Conversely, flies shifted down to 18[o]C immediately after training have normal long-term memory, whereas flies shifted down to 18[o]C 24 hours before training, but then shifted up to 29[o]C immediately after training, do not.

Expression of orb2^A.Scer\UAS in the mushroom body αβ neurons with the Scer\GAL4^c739 driver, in the α'β neurons with the Scer\GAL4^c305a, or in projection neurons with Scer\GAL4^GH146 does not rescue the long-term memory defects found in orb2^Δ/orb2^ΔQ mutants.

Long-term memory is rescued in orb2^Δ/orb2^ΔQ mutants where orb2^A.Scer\UAS is expressed in the γ neurons under the control of Scer\GAL4^Tab2-201Y, Scer\GAL4¹⁴⁷¹, or Scer\GAL4^H24.

(Keleman et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

94347

orb2^ΔQ

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Orb2Δ80Q

(Li et al., 2016)

orb2^delQ

(Hafer et al., 2011)

orb2^ΔQ

(Shao et al., 2023, Xu et al., 2014, Xu et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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