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General Information
Symbol
Dmel\orb2A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0241953
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-orb2A
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of the A isoform of orb2.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Expression of orb2A.Scer\UAS under the control of Scer\GAL4fru-GAL4, Scer\GAL4c747 or Scer\GAL4Mef2.247 restores 24 hour memory in orb2Δ/orb2ΔQ transheterozygotes. Using the TARGET system (using Scer\GAL80ts.alphaTub84B to suppress Scer\GAL4 expression a certain points during development) to express orb2A.Scer\UAS (under the control of Scer\GAL4Mef2.247) in the mushroom bodiesfully rescues long-term memory in orb2Δ/orb2ΔQ mutants when these flies are raised and tested at 29[o]C, but not when they are raised and tested at 18[o]C. Long-term memory is also restored when flies are raised at 18[o]C and then shifted to 29[o]C immediately after eclosion, thereby providing orb2 function only in the adults. The reciprocal experiment, in which flies are raised at 29[O]C, but shifted to 18[o]C as adults, does not restore long-term memory. Flies that are shifted up to 29[o]C just 2 hours before training show normal long-term memory, whereas those that are shifted immediately after training have no long-term memory. Conversely, flies shifted down to 18[o]C immediately after training have normal long-term memory, whereas flies shifted down to 18[o]C 24 hours before training, but then shifted up to 29[o]C immediately after training, do not. Expression of orb2A.Scer\UAS in the mushroom body αβ neurons with the Scer\GAL4c739 driver, in the α'β neurons with the Scer\GAL4c305a, or in projection neurons with Scer\GAL4GH146 does not rescue the long-term memory defects found in orb2Δ/orb2ΔQ mutants. Long-term memory is rescued in orb2Δ/orb2ΔQ mutants where orb2A.Scer\UAS is expressed in the γ neurons under the control of Scer\GAL4Tab2-201Y, Scer\GAL41471, or Scer\GAL4H24.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
orb2A.Scer\UAS
orb2A.UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)