Allele Dmel\orb2^B.Scer\UAS

General Information
Symbol	Dmel\orb2^B.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0241954
Feature type	allele	Associated gene	Dmel\orb2

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Keleman et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the B isoform of orb2. (Keleman et al., 2007)
Carried in construct	P{UAS-orb2.B} (Keleman et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^c747/orb2^B.Scer\UAS rescues orb2^ΔQ/orb2^Δ (Keleman et al., 2007) Scer\GAL4^fru-GAL4/orb2^B.Scer\UAS rescues orb2^ΔQ/orb2^Δ (Keleman et al., 2007) Scer\GAL4^Mef2.247, orb2^B.Scer\UAS, Scer\GAL4^Mef2.247 rescues orb2^ΔQ/orb2^Δ (Keleman et al., 2007)
Comments	Expression of orb2[B.Scer\UAS] under the control of Scer\GAL4[fru-GAL4], Scer\GAL4[c747] or Scer\GAL4[Mef2.247] restores 24 hour memory in orb2[Δ]/orb2[ΔQ] transheterozygotes. Using the TARGET system (using Scer\GAL80[ts.alphaTub84B] to suppress Scer\GAL4 expression a certain points during development) to express orb2[B.Scer\UAS] (under the control of Scer\GAL4[Mef2.247]) in the mushroom bodiesfully rescues long-term memory in orb2[Δ]/orb2[ΔQ] mutants when these flies are raised and tested at 29[o]C, but not when they are raised and tested at 18[o]C. Long-term memory is also restored when flies are raised at 18[o]C and then shifted to 29[o]C immediately after eclosion, thereby providing orb2 function only in the adults. The reciprocal experiment, in which flies are raised at 29[O]C, but shifted to 18[o]C as adults, does not restore long-term memory. Flies that are shifted up to 29[o]C just 2 hours before training show normal long-term memory, whereas those that are shifted immediately after training have no long-term memory. Conversely, flies shifted down to 18[o]C immediately after training have normal long-term memory, whereas flies shifted down to 18[o]C 24 hours before training, but then shifted up to 29[o]C immediately after training, do not. (Keleman et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	orb2^B.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Keleman et al., 2007, Nat. Neurosci. 10(12): 1587--1593 Function of the Drosophila CPEB protein Orb2 in long-term courtship memory. [FBrf0202837]

Allele Dmel\orb2B.Scer\UAS

Allele Dmel\orb2^B.Scer\UAS