Allele Dmel\CycE^WX

General Information
Symbol	Dmel\CycE^WX	Species	D. melanogaster
Name		FlyBase ID	FBal0241968
Feature type	allele	Associated gene	Dmel\CycE

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Wang and Kalderon, 2009)
Mutagen	ethyl methanesulfonate (Wang and Kalderon, 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2L:15,729,157..15,729,157 comment=AG to AA mutation in the splice acceptor of the last CycE intron. (Wang and Kalderon, 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference An "AG" to "AA" point mutation at the acceptor site of the last intron-exon junction which results in a frame-shift by one nucleotide. As a result, the last 91 amino acids of the wild-type gene product are changed to 31 new amino acids. The conserved "LTPP" destruction box is missing in CycE[WX] the protein. (Wang and Kalderon, 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	follicle stem cell \| somatic clone (Wang and Kalderon, 2009)
Detailed Description
	Statement Reference The percentage of ovarioles that retain CycE[WX] mutant follicle stem cell clones is lower than that of wild-type clones. In contrast, there is no significant difference in CycE[WX] mutant germ line stem cell retention compared to wild-type clones. (Wang and Kalderon, 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference CycE^WX has follicle stem cell \| somatic clone phenotype, enhanceable by dap^{Scer\UAS.cdNa}/Scer\GAL4^tub.PU (Wang and Kalderon, 2009)
Suppressed by
Statement Reference CycE^WX has follicle stem cell \| somatic clone phenotype, suppressible by dap^unspecified (Wang and Kalderon, 2009) CycE^WX has follicle stem cell \| somatic clone phenotype, suppressible by E2f^Scer\UAS.cNa/Dp^Scer\UAS.cDa/Scer\GAL4^tub.PU (Wang and Kalderon, 2009) CycE^WX has follicle stem cell \| somatic clone phenotype, suppressible by shg^Scer\UAS.cSa/Scer\GAL4^unspecified (Wang and Kalderon, 2009)
NOT suppressed by
Statement Reference CycE^WX has follicle stem cell \| somatic clone phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI/Scer\GAL4^tub.PU/th^Scer\UAS.cHa (Wang and Kalderon, 2009)
Additional Comments
Genetic Interactions
Statement Reference Heterozygosity for dap[unspecified] (a null allele of dap) partially rescues the persistence of CycE[WX] mutant follicle stem cells, measured 12 days after clone induction in both adults and larvae. Expression of Scer\GAL4[tub]-driven dap[Scer\UAS.cdNa] completely suppresses the formation of large transient CycE[WX] follicle cell clones. Scer\GAL4[tub]-driven co-expression of E2f[Scer\UAS.cNa] and Dp[Scer\UAS.cDa] in follicle stem cell (FSC) clones suppresses the CycE[WX]-associated defective FSC-maintenance phenotype. Scer\GAL4[tub]- or Scer\GAL4[Act5C.PI]-driven expression of th[Scer\UAS.cHa] does not suppress the loss of follicle stem cells in CycE[WX] mutant clones. Scer\GAL4[unspecified]-driven expression of shg[Scer\UAS.cSa] suppresses the loss of follicle stem cells in somatic clones of CycE[WX]. (Wang and Kalderon, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	CycE^WX is rescued by CycE^Scer\UAS.cWa/Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI/Scer\GAL4^tub.PU (Wang and Kalderon, 2009) CycE^WX is rescued by CycE^Scer\UAS.cWa/Scer\GAL4^tub.PU (Wang and Kalderon, 2009) CycE^WX is rescued by Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI/Scer\GAL4^tub.PU/CycE^{5A.Scer\UAS.cWa} (Wang and Kalderon, 2009) CycE^WX is rescued by Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI/Scer\GAL4^tub.PU/CycE^{WX.Scer\UAS.cWa} (Wang and Kalderon, 2009) CycE^WX is rescued by Scer\GAL4^tub.PU/CycE^{5A.Scer\UAS.cWa} (Wang and Kalderon, 2009) CycE^WX is rescued by Scer\GAL4^tub.PU/CycE^{WX.Scer\UAS.cWa} (Wang and Kalderon, 2009)
Not rescued by	CycE^WX is not rescued by CycE^{7A.Scer\UAS.cWa}/Scer\GAL4^tub.PU (Wang and Kalderon, 2009) CycE^WX is not rescued by Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI/CycE^{7A.Scer\UAS.cWa}/Scer\GAL4^tub.PU (Wang and Kalderon, 2009) CycE^WX is not rescued by Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI/Scer\GAL4^tub.PU/CycE^{RA.Scer\UAS.cWa} (Wang and Kalderon, 2009) CycE^WX is not rescued by Scer\GAL4^tub.PU/CycE^{RA.Scer\UAS.cWa} (Wang and Kalderon, 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CycE^WX cycE^WX (Wang and Kalderon, 2009)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Wang and Kalderon, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(51): 21701--21706 Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells. [FBrf0209757]

Allele Dmel\CycEWX

Allele Dmel\CycE^WX