Allele Dmel\Fmr1^{dsRNA.280.Scer\UAS}

General Information
Symbol	Dmel\Fmr1^{dsRNA.280.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0242104
Feature type	allele	Associated gene	Dmel\Fmr1

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Bolduc et al., 2008) in vitro construct - RNAi (Bolduc et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Two copies of sequence corresponding to bases 670-950 of Fmr1 are separated by a spacer sequence to generate an inverted repeat, driven by Scer\UAS regulatory sequences. (Bolduc et al., 2008)
Carried in construct	P{UAS-Fmr1.dsRNA.280} (Bolduc et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	memory defective, with Scer\GAL4^c747 (Bolduc et al., 2008) memory defective, with Scer\GAL4^ey-OK107 (Bolduc et al., 2008)
Phenotype Manifest In

Detailed Description
	Statement Reference Down-regulation of Fmr1 in the mushroom bodies through expression of Fmr1[dsRNA.280.Scer\UAS] under the control of Scer\GAL4[OK107] or Scer\GAL4[c747] leads to defects in 1-day memory after spaced training, but not after massed training. Notably, there is a decrease in FMRP levels on Kenyon cells, but no β lobe midline crossing. In contrast, knockdown of Fmr1 in the central complex (through Scer\GAL4[Feb170]-driven expression) or in the projection neurons from the anntennal lobe (though Scer\GAL4[GH146]-driven expression) does not yield defects in 1-d memory after spaced training. (Bolduc et al., 2008)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Fmr1^{dsRNA.280.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Bolduc et al., 2008, Nat. Neurosci. 11(10): 1143--1145 Excess protein synthesis in Drosophila Fragile X mutants impairs long-term memory. [FBrf0206065]

Allele Dmel\Fmr1dsRNA.280.Scer\UAS

Allele Dmel\Fmr1^{dsRNA.280.Scer\UAS}